ISOTOPE LABELING OF GLYCOPROTEIN GLYCANS FOR NMR OBSERVATION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 TSG-6是肿瘤坏死因子刺激基因-6的分泌产物，由一个Link模块和一个CUB模块结构域组成。 连接模块参与与糖胺聚糖的结合，整个系统以某种方式参与细胞外基质的重塑。 这是一个过程，通常发生在血管损伤，但也发生在类风湿性关节炎和骨关节炎。 TSG-6连接模块结合的特定糖胺聚糖包括透明质酸、硫酸乙酰肝素和硫酸软骨素，但似乎结合位点可能不同，并发生变构相互作用。戴教授提供了这种糖胺聚糖结合的质粒和表达建议 蛋白 该资源正在提供硫酸软骨素低聚物和NMR专业知识，以从结构上定义结合模式。 该项目将使用在资源中开发的专门方向限制来促进结构确定。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. TSG-6, the secreted product of tumor necrosis factor-stimulated gene-6, is composed of a Link module and a CUB module domain. The Link module is involved in binding to glycosaminoglycans and the entire system is in someway involved in remodeling the extracellular matrix. This is a process that normally occurs in response to injury of blood vessels but also occurs in rheumatoid arthritis and osteo-arthritis. The particular glycosaminoglycans to which the TSG-6 Link module binds include hyaluronan, heparan sulfate and chondroitin sulfate, but it appears that the binding sites might be different and interact allosterically. Professor Day has supplied the plasmid and expression advice on this glycosaminoglycan-binding protein. The Resource is providing the chondroitin sulfate oligomers and the NMR expertise to structurally define the binding mode. The project will use specialized orientational constraints developed in the Resource to facilitate structure determination.