Mosquito innate immune responses to arbovirus infections

参与蚊子对寄生虫和细菌感染的免疫反应的基因 经过广泛研究，但对病媒对病毒的反应的相似知识有限。我们的长期目标 是利用蚊子对病毒的反应知识来设计新的疾病控制方法。这项建议 研究重点是用于基因表达分析的全基因组平铺阵列的开发和使用 在蚊子媒介埃及伊蚊感染虫媒病毒后。由生成的信息 这项研究将揭示蚊子对一些病毒病原体的全球性和特异性反应，并提供 开发以遗传学为基础的疾病控制策略的材料。具体目标是：i) 设计包含Ae基因组询问探针的全基因组平铺阵列。埃及伊蚊，以及 虫媒病毒、登革热、黄热病和基孔肯雅热；2)鉴定蚊子蛋白编码和非编码 病毒感染后转录模式发生改变的RNA并验证其表达 利用定量基因扩增技术进行基因表达谱分析；3)设计转基因表达报告分子 病毒感染诱导的蚊子启动子的调控，并在体内测试控制的功能 序列。

The genes involved in mosquito immune responses to parasitic and bacterial infections have been studied extensively, however similar knowledge of vector responses to viruses is limited. Our long-term goal is to use knowledge of mosquito responses to viruses to design novel disease-control methods. This proposed research focuses on the development and usage of whole-genome tiling arrays for analyses of gene expression in the mosquito vector, Aedes aegypti, following infection with arboviruses. The information generated by this study will reveal global and specific responses of mosquitoes to a number of viral pathogens, and provide materials for the development of genetics-based disease control strategies. The Specific Aims are: i) to design whole-genome tiling arrays containing interrogation probes for the genomes of Ae. aegypti, and the arboviruses, Dengue, Yellow Fever and Chikungunya; 2) to identify mosquito protein-coding and noncoding RNAs with altered transcription patterns following infection with viruses and validate their expression profiles by quantitative gene amplification; and 3) to design transgenes expressing reporter molecules under the regulation of virus infection-induced mosquito promoters, and test in vivo the functionality of the control sequences.