Assessment Of Multiple Analytes In Women With Osteoporosis And Depression

评估患有骨质疏松症和抑郁症的女性的多种分析物

• 批准号: 7593823
• 负责人: Terry M. Phillips
• 金额: $ 1.85万
• 依托单位: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7593823
• 关键词: Adhesives; Affect; Age; Bandage; Biochemical Pathway; Biological Markers; Body Fluids; Bone Density; Bone Growth; Capillary Electrophoresis; Circadian Rhythms; Clinical; Code; Collection; Condition; Data; Data Analyses; Depressed mood; Devices; Double-Blind Method; Family; Growth Factor; Hour; Immune; Immunochemistry; Immunologic Markers; Individual; Inflammation; Inflammatory; Interleukin-6; Invasive; Left; Measures; Mental Depression; Modeling; Neuropeptides; Numbers; Osteoporosis; Pain; Pathway interactions; Pattern; Plasma; Postmenopause; Premenopause; Procedures; Process; Psyche structure; Purpose; Recovery; Recruitment Activity; Recycling; Role; Sampling; Severities; Skin; Stress; Sweat; Sweating; Techniques; Testing; Tissues; United States National Institutes of Health; Vacuum; Woman; body-mind; bone; bone loss; cohort; cytokine; design; desire; immunoaffinity chromatography; receptor; technique development

Bone loss in women pre- and post-menopause is a serious clinical condition affecting a reasonably large proportion of the public. Depression is a compounding condition that has been shown to accentuate pathways leading to bone loss and suggesting a mind-body connection. The POWER study conducted at the NIH Clinical Center is designed to study the interactions of depression and severity of osteoporosis in pre-menopausal women. The Ultramicro Analytical Immunochemistry group, NIBIB has undertaken to develop a panel of analytical techniques (recycling immunoaffinity chromatography RIC; immunoaffinity microarrays; immunoaffinity capillary electrophoresis ICE) to study a number of different systemic pathways that possibly may be involved in the connection between osteoporosis and depression. Cohorts of both normal and depressed age-matched women have been recruited into the double-blinded study. Codes were broken only after the analyses. Samples were taken on three occasions, during which 24 one-hour samples were taken and analyzed for 45 different analytes. Although the sample analyses have now been successfully completed, the data analysis is still in progress, due to the extremely large number of data points generated. Preliminary examination of the data has indicated circadian rhythms for many of the different cytokines and growth factors measured. The data also indicate that the depressed individuals can be classified into two groups according to their analyte patterns. Preliminary analysis has indicated a strong correlation between the presence of pain-associated neuropeptides and changes in bone density. Recent studies have focused on the development of techniques for measuring immune-stress biomarkers using minimally or non-invasive procedures. A commercially available sweat patch has been studied as a non-invasive 24-hour collection device. The patch, essentially a large adhesive bandage, is applied to the subjects skin and left in place for 24 hours during normal activities. The patch is removed and the collected sweat extracted. After considerable experimentation, it was found that a vacuum extraction device provided the most efficient means to recover the desired body fluid. Plasma samples were also drawn from the test subjects in order to compare analyte concentrations. The initial studies have shown that sweat recovery compares well with plasma concentrations and that a panel of neuro-immune markers of stress and inflammation can easily be measured using the analytical techniques already in place.

绝经前和绝经后妇女骨质流失是一种严重的临床状况，影响相当大比例的公众。抑郁症是一种复杂的情况，它被证明会加剧导致骨质流失的途径，并表明与身心有关。在美国国立卫生研究院临床中心进行的POWER研究旨在研究绝经前妇女抑郁和骨质疏松严重程度的相互作用。NIBIB的超微分析免疫化学组已经开发了一组分析技术（循环免疫亲和层析RIC；免疫亲和微阵列；免疫亲和毛细管电泳ICE）来研究可能涉及骨质疏松和抑郁症之间联系的许多不同的系统途径。这项双盲研究招募了年龄匹配的正常和抑郁女性。只有在分析之后，密码才被破解。样本采集了三次，在此期间，采集了24个一小时的样本，并对45种不同的分析物进行了分析。虽然现在样本分析已经成功完成，但是由于产生的数据点非常多，数据分析还在进行中。对数据的初步检查表明，测量了许多不同细胞因子和生长因子的昼夜节律。数据还表明，抑郁症患者可以根据他们的分析模式分为两组。初步分析表明，疼痛相关神经肽的存在与骨密度的变化之间存在很强的相关性。