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Ultrasound contrast agent dynamics and vascular effects

超声造影剂动力学和血管效应

基本信息

批准号：
7650424
负责人：
Thomas Matula
金额：
$ 46.89万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-05-11 至 2011-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7650424
关键词：
3-Dimensional Abbreviations Acoustics Affect Animals Attention Biological Blood Vessels Cell Membrane Permeability Contrast Media Data Data Collection Detection Diagnosis Diagnostic Diagnostic Imaging Dimensions Dose Drug Delivery Systems Ear Environment Erythrocytes Evans blue stain Evolution Extravasation FDA approved Fatigue Frequencies Gases Histology Image Imaging Techniques In Vitro Individual Intestines Lasers Lead Link Measurement Measures Mechanics Medical Medicine Mesentery Microbubbles Microbubbles Ultrasound Contrast Medium Microspheres Modeling Motion Oryctolagus cuniculus Pathway interactions Permeability Physiologic pulse Production Property Public Health Radial Radio Research Research Personnel Signal Transduction Speed Staging System Techniques Therapeutic Therapeutic Effect Uca Ultrasonography Vascular Endothelium Veins cardiography clinical application detector gene therapy improved in vivo indexing interest light scattering pressure programs research study response three-dimensional modeling

项目摘要

DESCRIPTION (provided by applicant): Ultrasound contrast agent microbubbles are composed of a variety of gases and stabilizing shell materials. FDA-approved applications of ultrasound contrast agents are currently limited to cardiography, and while their diagnostic utility is well-established, significant improvements could lead to increased clinical applications. In addition, the potential of cavitation-related bioeffects for therapeutic applications is exciting, and has received considerable recent attention; e.g., to facilitate targeted drug delivery or to enhance gene therapy, where changes in the vascular endothelium or cell membrane permeability are thought to be critical. The diagnostic and therapeutic effects of ultrasound contrast agents depend on the dynamical responses to the applied acoustic fields insonifying these microbubbles, either contained within the stabilizing shell, or in the form of free gas bubbles liberated from the contrast agent. The long-range objective of this project is to understand those responses and the permeabilizing effects they have on the vascular endothelium, so as to permit a rational exploitation of ultrasound-contrast-agent-enhanced cavitation. We propose to study bubble responses of ultrasound contrast agents in unconstrained media in-vitro, and in in-vivo intestinal mesentery blood vessels and ear veins. Bubble dynamics will be measured with laser light scattering and high-speed imaging, while cavitation will be measured with passive and active cavitation detectors. The biological effects of cavitation on the vascular endothelium will be examined through histology. Accordingly, the three specific aims of this project are as follows: (1) To image and to analyze the interaction of imaging and therapeutic ultrasound pulses with individual and clustered UCA microbubbles in vitro; (2) to assess cavitation in response to acoustic parameters for imaging and therapeutic applications; and (3) to determine how the constrained intravascular environment affects microbubble response ex vivo, and to determine its association with vascular permeabilization in vivo. This research is relevant to public health because understanding the interaction of ultrasound with designer bubbles will lead to improvements in medicine. These improvements will include a better diagnosis of medical problems, and therapeutic applications, such as delivery of drugs and medicine.

描述（由申请人提供）：超声造影剂微泡由多种气体和稳定外壳材料组成。FDA批准的超声造影剂的应用目前仅限于心动图，虽然其诊断实用性已得到充分证实，但显着的改进可能会导致临床应用的增加。此外，用于治疗应用的空化相关生物效应的潜力是令人兴奋的，并且最近已经受到相当大的关注;例如，以促进靶向药物递送或增强基因治疗，其中血管内皮或细胞膜渗透性的变化被认为是关键的。超声造影剂的诊断和治疗效果取决于对声穿透这些微泡的所施加的声场的动态响应，这些微泡包含在稳定壳内，或者以从造影剂释放的游离气泡的形式。本项目的长期目标是了解这些反应及其对血管内皮的透化作用，以便合理利用超声造影剂增强空化。我们建议研究气泡反应的超声造影剂在无约束的媒体在体外，并在体内肠肠系膜血管和耳静脉。气泡动力学将用激光散射和高速成像测量，而空化将用被动和主动空化探测器测量。将通过组织学检查空化对血管内皮的生物学效应。因此，本项目的三个具体目标如下：（1）成像和分析成像和治疗超声脉冲与单个和成簇的UCA微泡在体外的相互作用;（2）评估响应于成像和治疗应用的声学参数的空化;和（3）确定受约束的血管内环境如何影响离体微泡反应，并确定其与体内血管透化的关联。这项研究与公共卫生有关，因为了解超声波与设计师气泡的相互作用将导致医学的改进。这些改进将包括更好地诊断医疗问题，以及治疗应用，如药物和药物的输送。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Estimating the shell parameters of SonoVue microbubbles using light scattering.

DOI：
10.1121/1.3242346
发表时间：
2009-12
期刊：
The Journal of the Acoustical Society of America
影响因子：
0
作者：
J. Tu;J. Guan;Yuanyuan Qiu;T. Matula
通讯作者：
J. Tu;J. Guan;Yuanyuan Qiu;T. Matula

Agreement of the order of overall performance levels under different reading paradigms.

不同阅读范式下总体表现水平顺序的一致性。

DOI：
10.1016/j.acra.2008.07.011
发表时间：
2008
期刊：
Academic radiology
影响因子：
4.8
作者：
Gur,David;Bandos,AndriyI;Klym,AmyH;Cohen,CathyS;Hakim,ChristianeM;Hardesty,LaraA;Ganott,MarieA;Perrin,RonaldL;Poller,WilliamR;Shah,Ratan;Sumkin,JulesH;Wallace,LuisaP;Rockette,HowardE
通讯作者：
Rockette,HowardE

Microbubble sizing and shell characterization using flow cytometry.