Barcoded Nanowires for Multiplexed Clinical Diagnostics
Barcoded Nanowires for Multiplexed Clinical Diagnostics
批准号:
7568727
负责人:
CHRISTINE D KEATING
金额:
$27.42万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-08-04 至 2010-08-31
关键词:
AdenovirusesAntibiotic ResistanceAntibodiesArtsBacteriaBacterial InfectionsBiological AssayCaliberCell Culture TechniquesChemistryChlamydophila pneumoniaeClinicalCytomegalovirusDepositionDetectionDiagnosisDiagnosticDiffusionDirect immunofluorescenceElectromagneticsElectroplatingEnterovirusEvaluationFluorescenceGenerationsGrantHaemophilus influenzaeHaemophilus parainfluenzaeImageImmobilizationImmunoassayImmunofluorescence ImmunologicIndividualInfluenzaLabelLaboratoriesLengthMedicineMetalsMetapneumovirusMethodologyMethodsMicroscopeMolecularMonitorMycoplasma pneumoniaeOligonucleotidesOpticsParainfluenzaPathogen detectionPatientsPatternPreparationPropertyPublic HealthRecoveryResearch PersonnelRespiratory Tract InfectionsRespiratory syncytial virusRobotSamplingScreening procedureStreptococcus pneumoniaeSurfaceSuspension substanceSuspensionsSymptomsTechniquesTestingTimeTubeVial deviceViralVirusVirus SheddingWorkbasecollegedesignimprovedinstrumentationinterestnanometernanowirenucleic acid detectionparticlepathogenprogramsrespiratoryrespiratory virussuccessviral detectionvirology
中文摘要
我们建议在临床中发展快速的多重检测方法来诊断呼吸道病原体
英文摘要
We propose to develop rapid multiplexed assays for the diagnosis of respiratory pathogens in clinical
samples. Many viral and bacterial infections cause respiratory illnesses with very similar symptoms, thus,
timely and accurate pathogen identification is important for patient recovery and public health monitoring.
Current methods include direct immunofluorescence and cell culture, both of which currently require
individual tests for each pathogen. We will use barcoded metallic nanowires as encoded supports for two
types of multiplexed diagnostic assays, which will be tested on clinical samples and ultimately transitioned
into a clinical setting during the course of the proposed work.
Aim 1. Self quenching nanowire beacons. We will take advantage of the electromagnetic effects near metal
surfaces (quenching, enhancement, and alteration of fluorescence lifetimes), to produce nanowire beacon
probes that enable many assays to be conducted simultaneously in the same sample. The resulting
multiplexible, "closed tube" nucleic acid detection assays will require no target labeling or washing, nor any
special instrumentation beyond a conventional fluorescence optical microscope, and are therefore amenable
to point-of-contact assays such as clinical diagnostics. We propose here to develop a 17-plexed assay for
respiratory pathogens.
Aim 2. Simultaneous multiplexed amplification and immobilization for pathogen detection. For viruses shed
at very low levels, amplification (by growing in culture or by PCR) is necessary for detection. We will
overcome current limitations of viral detection by existing methods by introducing multiplexed amplification
and immobilization onto barcoded nanowires (MAIN). To accomplish this we will develop robust surface
attachment chemistry that can withstand thermocycling and will provide for minimal steric hindrance of
hybridization and extension, and will then use these advances to simultaneously direct all PCR products of
interest onto different, visually identifiable nanowire supports for sensitive pathogen detection.
期刊论文(21)
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DOI:
10.1021/la703854x
发表时间:
2008-08-19
期刊:
Langmuir : the ACS journal of surfaces and colloids
影响因子:
--
作者:
[Cederquist KB, Stoermer Golightly R, Keating CD]
通讯作者:
Keating CD
DOI:
10.1021/la1031703
发表时间:
2010-12-07
期刊:
Langmuir : the ACS journal of surfaces and colloids
影响因子:
--
作者:
[Cederquist KB, Keating CD]
通讯作者:
Keating CD
Assembly of gold nanowires by sedimentation from suspension: Experiments and simulation.
通过悬浮液沉降组装金纳米线:实验和模拟。
DOI:
10.1021/jp909251v
发表时间:
2010
期刊:
The journal of physical chemistry. C, Nanomaterials and interfaces
影响因子:
--
作者:
[Triplett,DerekA, Quimby,LisaM, Smith,BenjaminD, Rodríguez,DarimarHernández, StAngelo,SarahK, González,Pedro, Keating,ChristineD, Fichthorn,KristenA]
通讯作者:
Fichthorn,KristenA
DOI:
10.1126/science.1165921
发表时间:
2009-01-16
期刊:
Science (New York, N.Y.)
影响因子:
--
作者:
[Morrow TJ, Li M, Kim J, Mayer TS, Keating CD]
通讯作者:
Keating CD
DOI:
10.1021/la402362u
发表时间:
2013-09-10
期刊:
Langmuir : the ACS journal of surfaces and colloids
影响因子:
--
作者:
[Dean SL, Morrow TJ, Patrick S, Li M, Clawson GA, Mayer TS, Keating CD]
通讯作者:
Keating CD
共 11 条
Artificial Metabolons: Models for proximity-driven control over multienzyme pathw
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批准号:8050042
-
项目类别:
-
资助金额:$38.65万
-
财政年份:2009
-
负责人:CHRISTINE D KEATING
-
依托单位:
Artificial Metabolons: Models for proximity-driven control over multienzyme pathw
-
批准号:7784540
-
项目类别:
-
资助金额:$42.2万
-
财政年份:2009
-
负责人:CHRISTINE D KEATING
-
依托单位:
Artificial Metabolons: Models for proximity-driven control over multienzyme pathw
-
批准号:8237005
-
项目类别:
-
资助金额:$38.68万
-
财政年份:2009
-
负责人:CHRISTINE D KEATING
-
依托单位:
SELF BAR-CODED COLLOIDAL METAL NANOPARTICLES
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批准号:6603258
-
项目类别:
-
资助金额:$19.22万
-
财政年份:2000
-
负责人:CHRISTINE D KEATING
-
依托单位:
Barcoded Nanowires for Multiplexed Clinical Diagnostics
-
批准号:7096320
-
项目类别:
-
资助金额:$29.65万
-
财政年份:2000
-
负责人:CHRISTINE D KEATING
-
依托单位:
SELF BAR-CODED COLLOIDAL METAL NANOPARTICLES
-
批准号:6388353
-
项目类别:
-
资助金额:$20.42万
-
财政年份:2000
-
负责人:CHRISTINE D KEATING
-
依托单位:
Barcoded Nanowires for Multiplexed Clinical Diagnostics
-
批准号:7210612
-
项目类别:
-
资助金额:$29.59万
-
财政年份:2000
-
负责人:CHRISTINE D KEATING
-
依托单位:
SELF BAR-CODED COLLOIDAL METAL NANOPARTICLES
-
批准号:6536478
-
项目类别:
-
资助金额:$18.56万
-
财政年份:2000
-
负责人:CHRISTINE D KEATING
-
依托单位:
SELF BAR-CODED COLLOIDAL METAL NANOPARTICLES
-
批准号:6192593
-
项目类别:
-
资助金额:$27.29万
-
财政年份:2000
-
负责人:CHRISTINE D KEATING
-
依托单位:
ULTRARAPID DNA SEQUENCING BY SURFACE PLASMON RESONANCE
-
批准号:6181830
-
项目类别:
-
资助金额:$9.18万
-
财政年份:1999
-
负责人:CHRISTINE D KEATING
-
依托单位:
海外基金