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Mechanisms underlying cell-fate patterns in yeast communities

酵母群落细胞命运模式的潜在机制

基本信息

批准号：
9305292
负责人：
SAUL M HONIGBERG
金额：
$ 45.3万
依托单位：
UNIVERSITY OF MISSOURI KANSAS CITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-02 至 2020-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9305292
关键词：
Acetates Address Alleles Animal Model Biogenesis Biological Biological Assay Biology Buffers Cell Communication Cell Count Cell Wall Cells Chemicals Chromosome Mapping Communication Communities Dependency Development Diploidy Disease Environment Gene Expression Profile Genes Genetic Genetic Screening Goals Growth Health Hospitals Human Lead Malignant Neoplasms Measures Medical Device Meiosis Metabolic Metabolism Microbe Microbial Biofilms Microscopy Mitochondria Modernization Mycoses Nutrient Organism Pathogenicity Pathologic Pathway interactions Pattern Permeability Planets Population Reactive Oxygen Species Regulation Research Role Saccharomyces cerevisiae Saccharomycetales Signal Transduction System Temperature Testing Tissues Yeast Model System Yeasts biological adaptation to stress cell type combat community organizations driving force experimental study genome annotation innovation insight interest microbial community microorganism multi-photon mutant novel novel strategies respiratory response self organization transcription factor tumor two-dimensional

项目摘要

Project Summary / Abstract Communication likely first evolved on our planet as chemical signals between single-cell microorganisms. A fundamental purpose for this type of communication in modern microorganisms is to allow these microbes to self-organize into functional communities. Colonies of the budding yeast, S. cerevisiae, provide an opportunity to investigate this type of community organization due to this model organism's peerless genome annotation and genetic malleability. The Honigberg lab discovered that diploid yeast colonies are organized into a layer of feeder cells underlying a layer of meiotic (sporulating) cells. Furthermore, the relative number of cells and dimensions of the two layers depends on colony environment. Feeder cells may stimulate sporulation in the overall community by providing nutrients to the cells of the overlying layer. In addition to its scientific interest, the health relevance of the proposed research derives from the fact that the spatial organization of pathogenic yeast biofilms contributes significantly to the lethality of hospital-acquired fungal infections. Furthermore, mechanisms of microbial community organization could help elucidate the forces driving organization of tissues and tumors in humans. A long-range goal of the Honigberg lab is to identify the mechanisms that regulate the self- organization of yeast colonies from homogeneous to highly patterned communities. The first specific aim of the application is to test the “Differential Partitioning provides Environmental Buffer” hypothesis by determining whether the number of feeder cells in colonies correlates with the dependency on these feeder cells for sporulation across a range of conditions. In addition, we will determine whether establishing and maintaining differential partitioning depends on both cell autonomous and cell nonautonomous mechanisms. The second specific aim is to characterize feeder cells with respect to the similarity of expression patterns to quiescent cells found in cultures, and to test the hypotheses that the number of feeder cells in colonies responds to the respiratory state of the colony through mitochondrial signaling. Three complementary approaches are employed to address the above hypotheses. The first approach measures expression of known feeder-cell specific or sporulation-specific genes within intact wild- type or mutant colonies by confocal or multi-photon fluorescent microscopy, and to measure co-localization of these genes in cell populations from resuspended colonies. The second approach examines partitioning and other markers of colony development across a 2-D environmental landscape, i.e. when two environmental conditions (such as temperature or concentration of nutrients) are both varied. The third approach compares gene expression patterns in feeder and sporulation cell layers using FACS-Seq.

项目摘要/摘要通信可能最早是在我们的星球上作为单细胞微生物之间的化学信号进化而来的。一个现代微生物中这种交流的基本目的是让这些微生物自组织成功能社区。萌芽酵母的菌落，酿酒酵母，提供了一个有机会研究这种类型的社区组织，因为这种模式生物是无与伦比的基因组注释和遗传延展性。霍尼格伯格实验室发现，二倍体酵母菌落组织成一层滋养细胞，位于减数分裂(产孢子)细胞层的下面。此外，细胞的相对数量和两层的大小取决于菌落环境。滋养细胞可以通过向覆盖层的细胞提供营养物质来刺激整个群落中的产孢量。除了其科学意义外，拟议研究的健康相关性源于致病酵母菌生物膜的空间组织对致死性有很大贡献医院获得性真菌感染。此外，微生物群落的组织机制可以帮助阐明推动人类组织和肿瘤组织的力量。霍尼格伯格实验室的一个长期目标是识别调节自我- 酵母菌落的组织从同质到高度模式化的群落。的第一个具体目标应用程序是通过以下方式来测试“差异分区提供环境缓冲”假设确定菌落中饲养细胞的数量是否与对这些饲养细胞的依赖相关在一系列条件下形成孢子的细胞。此外，我们将确定是否建立和维持不同的划分依赖于细胞自主和细胞非自主机制。第二个具体目标是根据表达模式的相似性来表征饲养细胞在培养物中发现静止细胞，并检验集落中饲养细胞的数量通过线粒体信号对菌落的呼吸状态做出反应。本文采用了三种互补的方法来解决上述假设。第一方法测量已知的滋养细胞特异性或产孢量特异性基因在完整的野生- 通过共聚焦或多光子荧光显微镜观察类型或突变菌落，并测量共定位在重新悬浮的菌落中的细胞群体中。第二种方法检查分区以及在2-D环境景观中群体发展的其他标志，即当两个环境条件(如温度或营养物质浓度)都是不同的。第三方法使用FACS-Seq比较饲养层和孢子形成细胞层的基因表达模式。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Shrinking Daughters: Rlm1-Dependent G1/S Checkpoint Maintains Saccharomyces cerevisiae Daughter Cell Size and Viability.

缩小子细胞：Rlm1 依赖性 G1/S 检查点维持酿酒酵母子细胞大小和活力。

DOI：
10.1534/genetics.117.204206
发表时间：
2017
期刊：
Genetics
影响因子：
3.3
作者：
Piccirillo,Sarah;Neog,Deepshikha;Spade,David;VanHorn,JDavid;Tiede-Lewis,LeAnnM;Dallas,SarahL;Kapros,Tamas;Honigberg,SaulM
通讯作者：
Honigberg,SaulM

How Boundaries Form: Linked Nonautonomous Feedback Loops Regulate Pattern Formation in Yeast Colonies.

边界如何形成：链接的非自主反馈环路调节酵母菌落中的模式形成。

DOI：
10.1534/genetics.119.302700
发表时间：
2019
期刊：
Genetics
影响因子：
3.3
作者：
Piccirillo,Sarah;McCune,AbbigailH;Dedert,SamuelR;Kempf,CassandraG;Jimenez,Brian;Solst,ShaneR;Tiede-Lewis,LeAnnM;Honigberg,SaulM
通讯作者：
Honigberg,SaulM

Flo11p adhesin required for meiotic differentiation in Saccharomyces cerevisiae minicolonies grown on plastic surfaces.