The synergistic effects of climate and anthropogenic drivers on toxic cyanobacterial blooms

气候和人为驱动因素对有毒蓝藻水华的协同影响

• 批准号: 9789304
• 负责人: George S Bullerjahn
• 金额: $ 12.5万
• 依托单位: BOWLING GREEN STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9789304
• 关键词: Address; Affect; Agriculture; Algae; Algal Blooms; Biological Availability; Biomass; Budgets; Characteristics; Chemicals; Climate; Communities; Cues; Cyanobacterium; Data; Data Set; Development; Ecology; Environmental Health; Event; Exposure to; Fresh Water; Goals; Growth; Habitats; Health; Human; Metagenomics; Microbe; Microcystis; Molecular Biology; Natural Resources; Nature; Nitrates; Nitrogen; Nutrient; Organism; Pathway interactions; Physiology; Play; Population; Production; Research Priority; Research Project Grants; Risk; Role; Severities; Shapes; Source; Structure; Surveys; System; Systems Biology; Techniques; Temperature; Testing; Toxic effect; Toxin; Urea; Ursidae Family; Variant; Water; Water Supply; Work; anthropogenesis; climate change; cyanobacterial toxin; data management; data resource; design; environmental chemistry; experimental study; harmful algal blooms; hepatotoxin; human disease; interdisciplinary approach; member; metabolomics; metatranscriptomics; microbial community; microbiome; microbiota; microcystin; success; tool; transcriptomics; trigger point; waterborne

Project Summary Cyanobacterial bloom events are shaped by environmental cues that contribute to harmful algal bloom (cHAB) composition and toxigenicity. Indeed, in Lake Erie, two distinctly different cyanobacterial genera, Microcystis and Planktothrix, produce microcystin, a potent hepatotoxin. Whereas both cyanobacteria bloom in waters affected by nutrients delivered by agricultural runoff, Microcystis typically blooms in offshore waters, and Planktothrix dominates nearshore embayments. Prior work has suggested a role of climate-change induced temperature increases in promoting growth of Microcystis, and that Planktothrix persists in waters affected by prolonged nitrogen depletion. Given the different characteristics of these two organisms, limiting exposure to microcystin may require different management strategies to protect the water supply. Our overarching aim in this project is to address the extent to which N availability and temperature play in the development, toxigenicity and persistence of cHAB taxa in Lake Erie and other bloom-affected freshwaters. Informed by baseline data from metatranscriptomic surveys, we will design microcosm and chemostat experiments aimed at understanding competition between the two Lake Erie cHAB genera and factors contributing to bloom toxicity. Specifically, we will test three hypotheses in this project listed below: 1) Nitrogen availability controls cHAB community structure. Microcosms of mixed Microcystis and Planktothrix bloom communities will be exposed to different regimes of N availability and temperature to determine the degree to which each contribute to the dominance of one bloom-former over another. 2) Microcystin production is dependent on temperature and N speciation. Chemostat studies of Microcystis and Planktothrix cultures at control and warmer temperature will reveal how different chemical forms of N (nitrate vs. urea) promote growth and toxin production. 3) The proliferation of cHAB species is promoted by the activity of the limnetic microbiome. Metatransciptomic analysis of bloom communities will provide an assessment of the activities of the entire microbial consortium in cHAB events in order to determine what organisms and pathways assist in cHAB development, persistence and decline. A second aim of this project is to generate a comprehensive Lake Erie environmental `omics data set that can be shared and utilized by the integrated research projects within the Great Lakes Center for Fresh Waters and Human Health.

项目摘要 蓝细菌的花朵事件是由有害藻华（CHAB）的环境线索塑造的 成分和毒性。确实，在伊利湖中，两个截然不同的蓝细菌属，微囊藻 和Planktothrix，产生有效的肝毒素微囊蛋白。而两种蓝细菌在水中开花 受农业径流传递的营养影响，微囊藻通常在近海水域开花， Planktothrix占据了近海胚胎的主导地位。先前的工作提出了气候变化的作用 促进微囊藻的生长的温度升高，并且浮游生物持续存在于受影响 长时间的氮消耗。考虑到这两个生物的不同特征，限制了暴露于 微囊蛋白可能需要不同的管理策略来保护供水。我们的总体目标 该项目是为了解决开发中N的可用性和温度的播放程度， 伊利湖和其他受布鲁姆影响的淡水中的Chab分类单元的毒和持续性。通知 通过元转录组调查的基线数据，我们将设计缩影和化学固定实验 在了解两个伊利湖Chab属之间的竞争和导致开花毒性的因素。 具体而言，我们将在下面列出的该项目中检验三个假设： 1）氮的可用性控制CHAB社区结构。混合微囊藻和 Planktothrix Bloom社区将暴露于n个可用性和温度的不同制度 确定每种花样组合物比另一个花样的占主导地位的程度。 2）微囊蛋白的产生取决于温度和n种物种。微囊藻的化学稳定研究 在对照和温度较高的温度下，浮游物培养物将揭示不同的化学形式如何 N（硝酸盐与尿素）促进生长和毒素产生。 3）CHAB物种的增殖是由有限型微生物组的活性促进的。 Bloom社区的Metatransciptomic分析将对整个活动进行评估 CHAB事件中的微生物财团，以确定哪些生物和途径有助于CHAB 发展，持久性和衰落。 该项目的第二个目的是生成一个全面 由大湖鲜水中心内的综合研究项目共享和利用 人类健康。