Susceptibility determinants to Legionella pneumophila infection in smokers

吸烟者嗜肺军团菌感染的易感性决定因素

• 批准号: 10374758
• 负责人: Amal O Amer
• 金额: $ 19.5万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-18 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10374758
• 关键词: Achievement; Alveolar Macrophages; Bacteria; Bronchoalveolar Lavage; CRISPR/Cas technology; Cell Line; Cells; Cessation of life; Cigarette; Cytosine; DNA; DNA Methylation; Data; Disease; Disease Outbreaks; Elderly; Epigenetic Process; Gene Expression; Gene Expression Profile; Genes; Genomics; Human; Immune; Immunity; Individual; Infection; Innate Immune Response; Legionella; Legionnaires' Disease; Lentivirus; Life Cycle Stages; Literature; Lung; Measures; Methylation; Molecular; Morbidity - disease rate; Ohio; Outcome; Pathway Analysis; Pattern; Persons; Phagocytes; Pneumonia; Predisposition; Publications; Reporting; Research; Resolution; Risk; Sample Size; Sampling; Small Interfering RNA; Smoker; Smoking; Smoking History; Structure of parenchyma of lung; Testing; Time; Tobacco smoking behavior; Transcriptional Regulation; Transferase; Universities; Whole Blood; base; bisulfite sequencing; cigarette smoke; drinking water; epidemiology study; gene function; genome-wide analysis; high risk; insight; macrophage; methylomics; monocyte; mortality; non-smoker; novel; permissiveness; tobacco smoke exposure; transcriptome sequencing

Susceptibility determinants to Legionella pneumophila infection in smokers Abstract Legionella accounts for majority of drinking water-associated pneumonia outbreaks with high percentage of death. Legionella infection presents with a higher risk and worse outcome in the elderly, immune-compromised and smokers. Several epidemiological studies have demonstrated a strong association between tobacco smoking and an elevated risk of Legionella infection. However, the underlying mechanism is still unclear. To determine the mechanism by which smoking predisposes to Legionella infection, we will perform the following aims: Aim 1. Determine the methylomic and RNAseq profile of human alveolar macrophages from current smokers (>10 pack years) and non-smokers. Aim 2. Determine the function of genes with altered methylation and expression and identify those responsible for the increased susceptibility of macrophages to Legionella infection.

吸烟者嗜肺军团菌感染的易感性决定因素