Dynamics of RNA-protein interactions

项目摘要 我们的研究旨在描述RNA结合蛋白（RBP）及其相关蛋白的动态变化。 RNA代谢中的复合物。尽管对细胞功能至关重要，但RBP相互作用的动力学 对它们的底物了解甚少。为了解决这个问题，我们开发和应用新的 定量方法来表征体外和细胞中的动态RNA-蛋白质相互作用。计划 研究集中在三个问题：（i）RNA解旋酶Ded 1 p和DDX 3X的动力学如何 决定它们的细胞功能，（ii）TRAMP-外泌体机器的组分如何协调它们的细胞功能， 活性在体外和在细胞中，以及（iii）如何做RNA相互作用动力学的RBP与多种RNA 结合结构域决定细胞RBP功能。在这里，我们将重点介绍RBP IMP 1。计划 这项研究有望为RNA生物学提供全新的见解， 了解肿瘤发生和病毒感染性疾病的分子机制。

PROJECT SUMMARY Our research aims to delineate the dynamics of RNA binding proteins (RBPs) and their associated complexes in RNA metabolism. Although critical for cellular function, the dynamics by which RBPs interact with their substrates are poorly understood. To address this problem, we develop and apply novel quantitative approaches to characterize dynamic RNA-protein interactions in vitro and in cells. Planned research focuses on three questions: (i) how does the dynamics of the RNA helicases Ded1p and DDX3X dictate their cellular function, (ii) how do the components of the TRAMP-exosome machinery coordinate their activities in vitro and in the cell, and (iii) how do RNA-interaction dynamics of RBPs with multiple RNA binding domains determine cellular RBP function. Here we will focus on the RBP IMP1. The planned research is expected to provide fundamentally new insight into RNA biology with direct implications for understanding molecular mechanisms of tumorigenesis and viral infectious diseases.