Cerebral Protein Synthesis During Sleep and Memory Consolidation

在2018财年融资期间，我们解决了以下问题： 在沃尔特里德陆军研究所(T Balkin)和NINDS(D.Picchioni)的同事的合作下，我们应用了L-1-C-11亮氨酸正电子发射断层扫描(PET)方法来测量人类睡眠期间的RCP。为了解决睡眠恢复假说，我们测量了同一受试者在正常清醒、睡眠剥夺的清醒和睡眠中的RCP。我们假设RCP在睡眠期间增加，但在睡眠不足的清醒状态下，RCP保持在与休息清醒状态相当的水平。参与者在清醒、睡眠满足的状态下进行初始扫描。然后参与者在接下来的30小时内保持清醒，然后在睡眠不足但清醒的状态下接受第二次PET扫描。然后，参与者被鼓励在慢波睡眠期间接受第三次扫描时在扫描仪中睡觉。受试者为年龄在18岁到28岁之间的健康男性和女性志愿者。我们排除有神经和精神疾病、慢性疾病和睡眠障碍病史的受试者。我们的初步结果表明，在慢波睡眠期间，RCP没有差异。我们将继续撰写一份报告这些研究结果的手稿。

During the FY 2018 funding period, we addressed the following: In collaboration with our colleagues from the Walter Reed Army Institute of Research (T Balkin) and NINDS (D. Picchioni), we applied the L-1-C-11leucine positron emission tomography (PET) method to measure rCPS in humans during sleep. To address the restoration hypothesis of sleep, we measure rCPS in the same participant during normal wakefulness, sleep-deprived wakefulness, and sleep. We hypothesize that rCPS is increased during sleep, but that during sleep-deprived wakefulness, rCPS remain at levels comparable to rested wakefulness. Participants undergo the initial scan in the awake, sleep-sated state. Participants are then kept awake over the next 30 h and subsequently undergo a second PET scan in the sleep-deprived but awake state. Participants are then encouraged to sleep in the scanner while they undergo a third scan during slow wave sleep. Participants are healthy male and female volunteers between the ages of 18 and 28 y. We exclude participants with a history of neurological and psychiatric disorders, chronic medical conditions, and sleep disorders. Our preliminary results indicate no differences in rCPS during slow wave sleep. We continue to work on a manuscript reporting results of these studies.