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Genome Editing Shared Resource

基因组编辑共享资源

基本信息

批准号：
10024639
负责人：
MARK A MAGNUSON
金额：
$ 17.13万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-09-01 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10024639
关键词：
Animal Experimentation Animals Biological Breeding CRISPR/Cas technology Cancer Center Cancer Center Support Grant Cells Cellular biology Clustered Regularly Interspaced Short Palindromic Repeats Code Communities Complex Cryopreservation DNA Disasters Effectiveness Embryo Embryo Transfer Engineering Ensure Epitopes Experimental Designs Female Fertilization in Vitro Gene Deletion Gene Targeting Gene-Modified Generations Genes Genomic approach Genotype Goals Individual Injections Institution Insurance Knowledge Mammalian Oviducts Mediating Mentors Mentorship Methods Microinjections Mission Modeling Modification Molecular Biology Mus Mutant Strains Mice NCI Center for Cancer Research NIH Mouse Nonhomologous DNA End Joining Outcome Point Mutation Policies Preparation Proteins Publishing Reagent Reporting Reproducibility Reproductive Techniques Research Research Design Research Personnel Resource Sharing Resources Ribonucleoproteins Sampling Service delivery model Services Shipping Single-Stranded DNA Source Students Technology Tennessee Time Training Transgenic Mice Tumor Biology Universities Uterus Validation assisted reproduction blastocyst cost cost effective design embryo cryopreservation embryonic stem cell experimental study genome editing germ free condition improved medical schools member mouse model novel operation preservation programs pup reconstitution repaired repository responsible research conduct screening skills sperm cryopreservation success

项目摘要

CORE 008 – GENOME EDITING SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The mission of the Genome Editing Shared Resource (GESR), previously the Transgenic Mouse/Embryonic Stem Cell Shared Resource, is to enable the Vanderbilt-Ingram Cancer Center (VICC) research community, inclusive of our VICC members and partners at Meharry Medical College, Tennessee State University, and other NCI Cancer Centers to efficiently generate, store and share genetically-altered mouse models. GESR, which has been in existence for over 25 years, has undergone major changes over the past five years. Five prior service lines oriented around gene targeting in mouse embryonic stem cells (mESCs) became unnecessary after the discovery of CRISPR/Cas9. Thus, in late 2015, GESR shifted its focus towards improving the efficiency and outcomes of CRISPR/Cas9-mediated gene editing in one and two cell mouse embryos. Since then, over 800 mutant mice from 97 CRISPR/Cas9 experiments have been generated, with a 100% technical success rate for the past twenty-six projects. GESR now provides a full-service approach where the resource performs project design, pre-injection reagent validation, post-injection analysis of the resulting pups, and breeding of the first generation to provide the investigator with a correctly edited heterozygous mouse. Investigators no longer need extensive molecular biology skills, or even much knowledge about CRISPR/Cas9 gene editing strategies. To support the sharing of these new CRISPR-generated lines, GESR has become more proactive in encouraging the use of core cryopreservation services. In house cryopreservation of mouse lines improves colony management and enables specific pathogen free stocks to be sent as cryopreserved germplasm to other institutions. The resource continues to reconstitute cryopreserved mouse lines that are requested by VICC investigators from other investigators, or that exist within external repositories, by performing both in vitro fertilization and embryo transfer. Finally, the resource has established, and are striving to expand, a Vanderbilt Cryopreserved Mouse Repository (VCMR). The VCMR facilitates compliance with NIH Mouse Sharing Policies and enables storage and distribution of mice from Vanderbilt without the continued involvement of investigators. All microinjection and embryo transfer services are performed in a specific pathogen free barrier facility. Together, the combination of services provided by the GESR enables VICC members and other Vanderbilt investigators to efficiently generate and maintain novel genome-edited mouse models to study multiple aspects of cell and tumor biology using sophisticated mouse models.

Core 008 -基因组编辑共享资源