Mass Spectometry and Proteomics

质谱学和蛋白质组学核心技术综述 主要是电喷雾电离(ESI)液相色谱质谱联用技术的研究进展 串联质谱仪(LCMS)和串联质谱仪(MS/MS)的联用推动了 蛋白质组学和代谢组学领域。因为蛋白质组学和新陈代谢组学测量动态 细胞和动物的状态，它们是研究和发现一系列 从癌症到免疫学的病理生理状态。质谱学的主要目标是 蛋白质组学的核心是为VCIID-Cobre研究人员提供基于MASS的实验支持 蛋白质组学和代谢组学领域基于光谱学的分析。我们为以下项目提供支持 实验设计、样品制备、数据采集、数据分析和数据解释。 服务包括蛋白质组学分析，重点是蛋白质的量化和翻译后 修饰、小分子量化、代谢物模式识别以及速率和 使用代谢物和蛋白质的稳定同位素的动力学。这些服务有40多年的历史。 核心主任马修斯博士在这些领域领导核心设施方面的经验。弥撒 光谱学和蛋白质组学核心对VCIID-Cobre教员的成功起到了关键作用 VCIID-Cobre计划第一阶段和第二阶段期间出版物和赠款的关键信息。使用 第二阶段结束时的仪器扩展，增加了高分辨率Waters Xevo G2-XS ESI-LCMS/MS四极飞行时间仪和Waters Nano Acquity UPLC，内核已经能够 将其能力和服务扩展到第三阶段。核心第三阶段的目标是(1)提供 最先进的质谱学、尖端技术和经验丰富的人员，以增强 VCIID-COBRE的研究。除了已经进行的标准蛋白质分析外，VCIID-Cobre 教员们已经确定了他们未来研究所需的新技术。这些措施包括 代谢组学检测及代谢物检测平台的建立 使用稳定的同位素标记示踪剂的动力学和途径，并扩大我们的能力以提供 利用难以定义的翻译后修饰的稳定同位素标记进行鉴定和定量 和蛋白质周转率。(2)为VCIID-Cobre教职员工提供咨询服务基础设施 在设计、进行和解释实验以促进他们的研究和(3)开发一个长期的- 通过扩大用户基础、按服务收费结构、强大的机构来实现可持续发展的长期计划 支持和适应研究需求的变化。

SUMMARY for the Mass Spectrometry and Proteomics Core Developments in mass spectrometry, primarily electrospray ionization (ESI) liquid chromatography-mass spectrometry (LCMS) with tandem mass spectrometry (MS/MS) has powered the remarkable growth in the fields of both proteomics and metabolomics. Because proteomics and metabolomics measure the dynamic state of cells and animals, they are powerful techniques for studying and discovering mechanisms for a range of pathophysiologic states from cancer to immunology. The primary goal of the Mass Spectrometry and Proteomics Core is to provide VCIID-COBRE investigators with support for experiments based on mass spectrometry-based analyses in the areas of proteomics and metabolomics. We provide support for experimental design, sample preparation, data acquisition, data analysis, and interpretation of data obtained. Services include proteomic analyses with a focus on quantification of proteins and posttranslational modifications, small molecule quantification, metabolite pattern identification, and measurement of rates and kinetics using stable isotopes of both metabolites and proteins. These services are based upon over 40 years of experience in these areas leading core facilities by the Core Director, Dr. Matthews. The Mass Spectrometry and Proteomics Core has been pivotal to the success of VCIID-COBRE faculty by contributing critical information for publications and grants during Phases I and II of the VCIID-COBRE program. With instrumentation expansion at the end of Phase II with the addition of a high resolution Waters Xevo G2-XS ESI-LCMS/MS quadrupole-time of flight instrument and Waters nanoAcquity UPLC, the Core has been able to expand its capabilities and services going into Phase III. The aims of the Core for Phase III are (1) to provide state-of-the-art mass spectrometry, cutting-edge techniques, and experienced personnel to enhance the research of the VCIID-COBRE. In addition to the standard protein analyses already conducted, VCIID-COBRE faculty has identified new techniques that are required for their future investigations. These include development of a broad-based platform for metabolomics measurements and measurement of metabolite kinetics and pathways using stable isotopically labeled tracers and broadening our capabilities to provide identification and quantification using stable isotope labels of difficult to define posttranslational modifications and rates of protein turnover. (2) To provide an infrastructure of consultative services to VCIID-COBRE faculty in the design, conduct, and interpretation of experiments to facilitate their research and (3) To develop a long- term plan for sustainability through expansion of user base with a fee-for-service structure, strong institutional support, and adaptation to changes in research needs.