VERMONT IMMUNOBIOL / INFECTIOUS DISEASES CTR: CORE C: PROTEOMICS ANALYSIS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这一核心将为Cobre调查人员提供蛋白质组学分析服务和技术援助。核心将由医学和化学系的德怀特·马修斯和微生物学和分子遗传学系的加里·沃德指导。马修斯博士负责核心的整体工作，并将重点放在核心的质谱学方面。马修斯博士在质谱学方面有几十年的历史，并在密歇根大学的几个地点指导质谱学仪器。沃德博士将专注于蛋白质分离和分离技术。显然，如果要测量的样品不能以可行的形式分离出来，质谱学的力量就会丧失。此外，沃德博士还将负责蛋白质的二维(2D)凝胶电泳法分离。这种技术在核心中作为一种独立的技术是有价值的。Ward博士在蛋白质纯化和分离方面拥有丰富的经验，包括各种层析技术和2D凝胶电泳。还将通过该核心以技术人员的形式专门提供技术专业知识，该技术人员将负责通过质谱仪运行样品以及制备样品和2D凝胶分离。技术人员将处理许多调查人员没有时间和专业知识来管理的实际任务。技术人员还将协助培训感兴趣的调查人员和学生如何执行运行他们自己的样本所需的技术。将为调查人员制定程序，以确保他们将获得对他们的实验和方案最有利的结果。马修斯博士和沃德博士将在从计划实验到解释结果的所有阶段直接与调查人员合作。蛋白质组学技术是复杂的，充满了微妙的复杂性，核心将在这方面帮助用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core will provide proteomics analytical services and technical assistance to the COBRE investigators. The core will be directed by Dwight Matthews, Departments of Medicine & Chemistry and by Gary Ward, Department of Microbiology & Molecular Genetics. Dr. Matthews is responsible overall for the core and will focus on the mass spectrometry aspects of the core. Dr. Matthews has a several-decade long history in mass spectrometry and directs mass spectrometry instrumentation in several locations at UVM. Dr. Ward will focus on protein isolation and separation techniques. Clearly, the power of mass spectrometry is lost if the samples to be measured cannot be isolated in a viable form. In addition, Dr. Ward will be responsible for 2-dimensional (2D) gel electrophoresis separation of proteins. This technique is valuable as a stand-alone technique in the core. Dr Ward has extensive experience in protein purification and separation, including a variety of chromatographic techniques and 2D gel electrophoresis. Technical expertise will also be available specifically through this core in the form of a technical person who will be responsible for both running samples by mass spectrometry and for preparing samples and 2D gel separations. The technical person will handle many of the hands-on tasks that the investigators will not have the time and expertise to manage. The technical person will also assist in training interested investigators and students how to perform the techniques required to run their own samples. Procedures will be in place for investigators to assure that they will obtain results that are optimal for their experiments and protocols. Both Drs. Matthews and Ward will work directly with the investigators at all stages from planning experiments to interpreting results. The proteomics techniques are complicated and fraught with subtle complexities, and the core will assist users in this regard.