A nano ESI-LCMS/MS Waters XEVO G2-S QTOF for UVM

用于 UVM 的纳米 ESI-LCMS/MS Waters XEVO G2-S QTOF

• 批准号: 8640621
• 负责人: Dwight E Matthews
• 金额: $ 46.16万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640621
• 关键词: Area; Biochemistry; Blood coagulation; Chemistry; Diet; Drug Design; Enzymes; Glycoproteins; Health; Ions; Measures; Medicine; Microbiology; Molecular Genetics; Organism; Oxonia Active; Parasites; Post-Translational Protein Processing; Proteins; Research; Research Personnel; Resolution; Science; System; Thrombosis; Universities; Vermont; Virus; Water; base; college; combat; hydronium ion; instrument; mass spectrometer; nano; oxidation

DESCRIPTION (provided by applicant): We propose to purchase a Waters Xevo G2-S QTOF high resolution ESI LCMS/MS instrument with a Waters nanoACQUITY UHPLC system to advance research at the University of Vermont (UVM) in several key areas of biomedical science. Although LCMS instruments exist at UVM, most are low resolution mass spectrometers: four ion trap analyzers and one triple quadrupole. The one high resolution LTQ-Orbitrap Discovery LCMS lacks several capabilities that are needed by investigators. We are requesting a complementary high resolution QTOF instrument that would add the following capabilities to our campus and to the state of Vermont: ability to measure ions at high resolution above m/z 2000~ ability to measure at high resolution low mass ions in MS/MS spectra that contain marker immonium and oxonium ions~ ability to acquire rapidly high resolution spectra appropriate for nanoUHPLC applications~ ability to measure whole proteins with sufficient resolution to be able to discern mass differences due to oxidation or addition of posttranslational modifications~ and ability to perform automated glycoprotein analysis. Users of the instrument span multiple departments and colleges (Biochemistry, Chemistry, Medicine, and Microbiology & Molecular Genetics). In addition the instrument would be available to a wider campus user base through the shared instrument facility in the Department of Chemistry. The major users of the instrument would benefit and advance research in understanding and combating pathogenic organisms ranging from parasites to hemorrhagic viruses, blood clotting and treating thrombosis, understanding enzyme systems leading to drug design, and understanding the effect of diet upon health.

描述(由申请人提供)：我们建议购买Waters Xevo G2-S QTOF高分辨率ESI LC MS/MS仪器，配备Waters Nano ACQUITY UHPLC系统，以促进佛蒙特州大学在生物医学科学几个关键领域的研究。尽管密歇根州立大学有LC MS仪器，但大多数都是低分辨率质谱仪：四个离子陷阱分析器和一个三重四极杆。一个高分辨率的LTQ-Orbitrap Discovery LCMS缺乏调查人员所需的几个能力。我们需要一个补充的高分辨率QTOF仪器，它将为我们的校园和佛蒙特州增加以下能力：能够在m/z 2000以上的高分辨率下测量离子~能够在高分辨率下测量MS/MS光谱中包含标志性金属离子和氧离子的低质量离子~能够快速获得适合于纳米UHPLC应用的高分辨率光谱~能够以足够的分辨率测量整个蛋白质的能力，能够分辨由于氧化或翻译后添加而导致的质量差异 改进~以及执行自动化糖蛋白分析的能力。该仪器的用户跨越多个系和学院(生物化学、化学、医学和微生物学与分子遗传学)。此外，该仪器将通过化学系的共用仪器设施向更广泛的校园用户群提供。该仪器的主要用户将在了解和抗击从寄生虫到出血性病毒的病原体、凝血和治疗血栓形成、了解导致药物设计的酶系统以及了解饮食对健康的影响方面受益并推进研究。

项目成果

Mechanism and Dynamics of Intramolecular C-H Insertion Reactions of 1-Aza-2-azoniaallene Salts.

• DOI: 10.1021/jacs.5b04474
• 发表时间: 2015-07-22
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Hong X;Bercovici DA;Yang Z;Al-Bataineh N;Srinivasan R;Dhakal RC;Houk KN;Brewer M
• 通讯作者: Brewer M

Intramolecular (4 + 2) cycloaddition of aryl-1-aza-2-azoniaallene salts: A practical approach to highly sterically-congested polycyclic protonated azomethine imines.

芳基-1-氮杂-2-azoniaallene盐的分子内(4 2)环加成：高度空间拥挤的多环质子化偶氮甲碱亚胺的实用方法。

• DOI: 10.1016/j.tet.2016.03.037
• 发表时间: 2016
• 期刊: Tetrahedron
• 影响因子: 2.1
• 作者: Dhakal,RamC;Brewer,Matthias
• 通讯作者: Brewer,Matthias