Optical Mapping of Electromechanics of the Stomach

胃机电光学测绘

• 批准号: 10007967
• 负责人: Jack M Rogers
• 金额: $ 31.18万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-17 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10007967
• 关键词: Animal Experiments; Animal Model; Animals; Arrhythmia; Basic Science; Biomedical Engineering; Body Surface; Cells; Characteristics; Chronic; Collaborations; Complex; Coupled; Data; Diagnosis; Dimensions; Disease; Dyes; Dyspepsia; Electrodes; Family suidae; Fluorescence; Fluorescent Dyes; Functional disorder; Future; Gastric Emptying; Gastrointestinal Motility; Gastrointestinal Physiology; Gastroparesis; Goals; Healthcare; Heart; Heterogeneity; Human; Image; Interstitial Cell of Cajal; Knowledge; Laparotomy; Light; Liquid substance; Maps; Mechanics; Medicine; Membrane Potentials; Methods; Modeling; Monitor; Morphologic artifacts; Motion; Motor Activity; Muscle Contraction; Nausea and Vomiting; Operative Surgical Procedures; Optics; Organ; Patients; Pattern; Pharmacology; Phase; Physiology; Play; Preparation; Recovery; Refractory; Research; Research Personnel; Research Project Grants; Resolution; Role; Smooth Muscle Myocytes; Speed; Stains; Stomach; Stomach Content; Stretching; Surface; System; Tail; Techniques; Technology; Testing; Tissues; Universities; Work; base; experience; gastrointestinal; gastrointestinal system; improved; in vivo; instrumentation; member; motility disorder; new technology; novel; optical imaging; response; spatiotemporal; stomach motility; success; tool

The stomach is an electromechanical organ whose contractions are coordinated by an electrical wave called the slow wave. This activity is traditionally studied at low spatial resolution by recording from sparse electrodes either in contact with the organ or on the body surface. Recently, a few groups have mapped slow wave propagation with much higher spatial resolution by placing arrays of dozens of electrodes on the stom- ach’s surface. These studies are revealing the details of normal slow wave propagation as well as dysrhythmic patterns that occur in disease states. However, there is little information on how normal and abnormal slow waves relate to detailed spatial patterns of muscle contraction. Improved understanding is needed to improve diagnosis and treatment of debilitating gastric motility disorders that are underdiagnosed and understudied. To this end, this Bioengineering Research Grant will develop novel research instrumentation to simultane- ously record from the stomach: (1) spatiotemporal patterns of electrical activation and recovery and (2) spatio- temporal patterns of muscle contraction. The methods will be used as research tools in animal experiments. Aim 1. Develop a system to image the membrane potential (Vm) of smooth muscle cells on the stomach’s surface. In an in vivo swine model, the stomach will be exposed and stained with a fluorescent dye whose re- sponse is modulated by Vm. Small fiducial markers will be attached to the serosal surface. Fluorescence emis- sion will be imaged with a video camera. By tracking the motion of the fiducial markers and alternating the wavelength of excitation light delivered with each camera frame, motion artifact caused by stomach contraction will be suppressed. These data will enable us to track slow waves as they propagate across the stomach. Aim 2: Develop an optical system to image the mechanical contraction that results from slow wave propa- gation. Additional video camera(s) mounted in a binocular fashion will be used to track the motion of the fidu- cial markers in three dimensions. From this, the deformation of the stomach’s surface will be quantified in terms of finite strain. These data will be temporally synchronized with the Vm data. These data will enable de- tailed study of the interactions between electrical and mechanical function in the stomach. Aim 3: Perform combined electromechanical mapping studies in normal preparations and preparations in which abnormal slow wave propagation patterns (dysrhythmias) are induced pharmacologically. Gastric empty- ing of liquid test meals will be compared with electromechanical mapping data during normal and dysrhythmic slow wave propagation. The project is a collaboration between the PI, who is an expert in developing and applying novel optical mapping methods for the heart, and Auckland-based investigators, who are experts in gastrointestinal physiol- ogy and mapping. We expect that success of this project will lead to future collaborations applying the new technology to problems in physiology and medicine.

胃是一个机电器官，它的收缩是由电波协调的