Optical Mapping of Electromechanics of the Stomach

胃机电光学测绘

• 批准号: 10307603
• 负责人: Jack M Rogers
• 金额: $ 32.9万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10307603
• 关键词: 3-Dimensional; Animal Experiments; Animal Model; Animals; Arrhythmia; Basic Science; Biomedical Engineering; Body Surface; Cells; Characteristics; Chronic; Collaborations; Complex; Coupled; Data; Diagnosis; Disease; Dyes; Dyspepsia; Electrodes; Family suidae; Fluorescence; Fluorescent Dyes; Functional disorder; Future; Gastric Emptying; Gastrointestinal Motility; Gastrointestinal Physiology; Gastroparesis; Goals; Healthcare; Heart; Heterogeneity; Human; Image; Interstitial Cell of Cajal; Knowledge; Laparotomy; Light; Liquid substance; Maps; Mechanics; Medicine; Membrane Potentials; Methods; Monitor; Morphologic artifacts; Motion; Motor Activity; Muscle Contraction; Nausea and Vomiting; Operative Surgical Procedures; Optics; Organ; Patients; Pattern; Pharmacology; Phase; Physiology; Play; Preparation; Recovery; Refractory; Research; Research Personnel; Research Project Grants; Resolution; Role; Smooth Muscle Myocytes; Speed; Stains; Stomach; Stomach Content; Stretching; Surface; System; Tail; Techniques; Technology; Testing; Tissues; Universities; Work; base; experience; gastrointestinal; gastrointestinal system; improved; in vivo; instrumentation; member; motility disorder; new technology; novel; optical imaging; porcine model; response; spatiotemporal; stomach motility; success; tool

The stomach is an electromechanical organ whose contractions are coordinated by an electrical wave called the slow wave. This activity is traditionally studied at low spatial resolution by recording from sparse electrodes either in contact with the organ or on the body surface. Recently, a few groups have mapped slow wave propagation with much higher spatial resolution by placing arrays of dozens of electrodes on the stom- ach’s surface. These studies are revealing the details of normal slow wave propagation as well as dysrhythmic patterns that occur in disease states. However, there is little information on how normal and abnormal slow waves relate to detailed spatial patterns of muscle contraction. Improved understanding is needed for better diagnosis and treatment of debilitating gastric motility disorders that are underdiagnosed and understudied. To this end, this Bioengineering Research Grant will develop novel instrumentation to simultaneously rec- ord spatiotemporal patterns of (1) gastric electrical activation and recovery and (2) stomach muscle contrac- tion. At this stage, the methods will be used as research tools in animal experiments. Aim 1. Develop a system to image the membrane potential (Vm) of smooth muscle cells on the stomach’s surface. In an in vivo swine model, the stomach will be exposed and stained with a fluorescent dye whose re- sponse is modulated by Vm. Small fiducial markers will be attached to the serosal surface. Fluorescence emis- sion will be imaged with a video camera. By tracking the motion of the fiducial markers and alternating the wavelength of excitation light delivered with each camera frame, motion artifact caused by stomach contraction will be suppressed. These data will enable us to track slow waves as they propagate across the stomach. Aim 2: Develop an optical system to image the mechanical contraction that results from slow wave propa- gation. Additional video camera(s) mounted in a binocular fashion will be used to track the motion of the fidu- cial markers in three dimensions. From this, the deformation of the stomach’s surface will be quantified in terms of finite strain. These data will be temporally synchronized with the Vm data. These data will enable de- tailed study of the interactions between electrical and mechanical function in the stomach. Aim 3: Perform combined electromechanical mapping studies in normal preparations and preparations in which abnormal slow wave propagation patterns (dysrhythmias) are induced pharmacologically. Gastric empty- ing of liquid test meals will be compared with electromechanical mapping data during normal and dysrhythmic slow wave propagation. This project is a continuation of the successful collaboration between the PI, who is an expert in developing and applying novel optical instrumentation for coupled electromechanics, and Auckland-based investigators, who are experts in gastrointestinal physiology and electrical mapping. We expect that success of this project will lead to future projects applying the new technology to problems in physiology and medicine.

胃是一个机电器官，其收缩由电波协调 称为慢波。这种活动传统上是在低空间分辨率研究，记录从稀疏 与器官接触或在身体表面上的电极。最近，一些组织在绘制地图时 波传播具有更高的空间分辨率，通过将几十个电极的阵列放置在stom， 每个人的表面。这些研究揭示了正常慢波传播的细节，以及节律失调 在疾病状态下发生的模式。然而，很少有关于正常和异常缓慢的信息 波与肌肉收缩的详细空间模式有关。需要更好地理解 诊断和治疗未被充分诊断和研究的衰弱性胃动力障碍。 为此，这项生物工程研究补助金将开发新的仪器，同时recc- 顺序（1）胃电激活和恢复和（2）胃肌肉收缩的时空模式， 是的。在这个阶段，这些方法将被用作动物实验的研究工具。 目标1。开发一个系统来成像胃的平滑肌细胞的膜电位（Vm）， 面在体内猪模型中，将暴露胃并用荧光染料染色，其重新染色。 sponse由Vm调制。将小的基准标记物附着在锯齿表面。荧光发射率 将用摄像机对锡永进行成像。通过跟踪基准标记的运动并交替 每个摄像机帧传递的激发光的波长，由胃收缩引起的运动伪影 将被压制。这些数据将使我们能够跟踪慢波，因为它们在胃中传播。 目的2：开发一个光学系统，以成像的机械收缩，结果从慢波propa。 gation。以双目方式安装的额外摄像机将用于跟踪fidu的运动， 三维的社会标志由此，胃表面的变形将被量化， 有限应变项。这些数据将与Vm数据在时间上同步。这些数据将使德- 尾研究电和机械功能之间的相互作用在胃。 目的3：在正常制剂和非正常制剂中进行联合机电标测研究。 其异常慢波传播模式（节律障碍）是由电刺激引起的。胃排空- 将液体试验餐的摄入与正常和节律紊乱期间的机电标测数据进行比较。 慢波传播 该项目是PI之间成功合作的延续，PI是开发 并将新型光学仪器应用于机电耦合，奥克兰的研究人员， 他们是胃肠道生理学和电图方面的专家。我们期待这个项目的成功 将导致未来的项目将新技术应用于生理学和医学问题。