Bioinformatics Studies of Human Viruses

人类病毒的生物信息学研究

• 批准号: 10007524
• 负责人: John Spouge
• 金额: $ 59.42万
• 依托单位: NATIONAL LIBRARY OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10007524
• 关键词: American; Bioinformatics; Clinical; Data; Databases; Epidemic; HIV; Human; Infection; Methods; Proteins; RNA; SIV; Viral; Viral Physiology; Viral Proteins; Virion; Virus; computerized tools; env Gene Products; nef Genes; statistics; virology

Having studied the statistics of the specific signature residues in the Env protein for SIV infection, and having determined by computation that the founder viruses of the SIV infection differ significantly from the typical virions in the stock inoculum, we examined the changes in the Nef gene and its RNA during the North American HIV epidemic. Using clinical sequence data, Dr Manzourolajdad discovered that the Nef gene had systematically evolved and increased the possibility of alternative folds in Nef RNA. Consequently, we developed general computational tools for identifying alternative folds in riboswitches, to aid in the identification of switches and the corresponding RNA substructures.

在研究了SIV感染的包膜蛋白中特异性特征残基的统计数据，并通过计算确定SIV感染的创始人病毒与股票接种物中的典型病毒粒子显著不同后，我们检测了北美HIV流行期间Nef基因及其RNA的变化。利用临床序列数据，Manzourolajda博士发现Nef基因已经系统地进化，并增加了Nef RNA中可选折叠的可能性。因此，我们开发了通用的计算工具来识别核糖开关中的替代折叠，以帮助识别开关和相应的RNA亚结构。