TAS::75 0849::TAS INCREMENTAL FUNDING

支持是与CCR （Farrar实验室）联合研究计划的一部分，该计划旨在研究各种食物成分作为肿瘤干细胞行为调节剂的作用，并最终对肿瘤再生产生影响。这些体外研究使用细胞培养技术来评估食物成分的生理相关浓度对癌性和非癌性干细胞分化的影响。通过多种生物学指标评价吲哚- 3-甲醇、染料木素、白藜芦醇、烯丙基硫等对干细胞分化和生长潜能的影响。这项支持是与CCR（博士）联合研究计划的一部分。Timothy Veenstra和Thomas Turbyville实验室)使用蛋白质组学技术来评估各种食物成分作为乳腺癌风险和肿瘤行为调节剂的作用。使用培养细胞的体外研究和动物研究都将用于评估食品成分。最初的研究将集中在染料木素作为正常细胞和肿瘤细胞的修饰剂。未来的研究将评估吲哚- 3-甲醇、白藜芦醇对蛋白质组学变化的影响，以及这些变化与细胞增殖的关系。这项支持是与CCR（科尔本实验室）联合研究计划的一部分，旨在研究各种食物成分作为炎症调节剂的作用，并最终对结肠癌产生影响。结合成像技术的动物研究被用来监测饮食的影响。通过几种生物学测量来评价吲哚- 3-甲醇、染料木素、白藜芦醇、黄酮类化合物等对炎症过程的影响。Fisher Bio Services接收来自贡献实验室的临床样本，将指定的样本分成若干份以构建参考集，将每个样本的标识符记录到数据库中，将所有这些样本保存在冰柜中，并将参考集发送给经批准进行测试的科学家。只要样品还在冰柜里，这将是一个持续的项目。在临床试验期间收集的血液和宫颈细胞样本的储存和检索。宫颈小瓶储存在一个寒冷的房间里。其他血液和子宫颈样本则储存在液氮冷冻机中。这笔资金用于补偿Frederick完成人类和啮齿动物模型样本的转录组学（mRNA）分析。血液和组织样本将提供给进行转录组分析的人员。所提供的大多数样品都需要初始提取。这些分析的数据以电子方式提供给我们进行独立分析。SELECT生物储存库收集了来自SELECT临床试验的血液、脚趾甲、白细胞和血浆，涉及35000多名男性。正在进行的活动包括处理在过渡访问中看到的2500名男性的血液，储存自2000年研究开始以来的所有血液，每周生成BSI文件，与SELECT统计中心人员进行持续沟通，从粘附子研究中运送样本，提取大约400个样本的DNA。NSABP STAR储存库包括正在进行的标本储存和冷冻库的维护，这些冷冻库储存了参加STAR临床试验的19000多名妇女的血液、白细胞和血浆。这些任务包括定期检查标本和冷冻室、标本的加入、记录的储存以及与NSABP的互动。

Support is part of a joint research initiative with CCR (Farrar Laboratory) to examine the role of various food components as modifiers of the behavior of tumor stem cells and ultimately on tumor regeneration. These in vitro studies use cell culture technologies to evaluate the influence of physiologically relevant concentrations of food components on the differentiation of cancerous and non-cancerous stem cells. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, allyl sulfur, etc. on stem cell differentiation and growth potential. This support is part of a joint research initiative with CCR (Drs. Timothy Veenstra and Thomas Turbyville Laboratories) to examine use proteomic technologies to evaluate the role of various food components as modifiers of breast cancer risk and tumor behavior. Both in vitro studies using cells in culture and animal studies will be used to evaluate food components. Initially studies will focus on genistein as a modifier of both normal and tumor cells in culture. Future studies will evaluate the influence of indole 3-carbinol, resveratrol on shifts in proteomics and how these relate to cell proliferation. This support is part of a joint research initiative with CCR (Colburn Laboratory) to examine the role of various food components as modifiers of inflammation and ultimately the impact on colon cancer. Animal studies which incorporate imaging technologies are used to monitor the impact of diet. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, flavonoids in beans, etc. on the inflammatory process. Fisher Bio Services receives clinical samples from contributing labs, aliquots specified samples to construct reference sets, logs identifiers for each sample, into their database, maintains freezers all these samples and sends out reference sets to scientists approved for testing. This will be an ongoing project as long as samples remain in the freezers. Storage and retrieval as needed of blood and cervical cell samples that were collected during clinical trial � ALTS. Cervical vials are stored in a cold room. Other blood and cervical samples are stored in liquid nitrogen freezers. This support is to reimburse Frederick for completing transcriptomic (mRNA) analysis from samples from human being and rodent models. Blood and tissue samples will be made available to those conducting the transcriptomic analysis. Most of the samples provided will require initial extraction. The data from these analyses are provided to us electronically for independent analysis. The SELECT Biorepository is a collection of blood, toenails, white cells, and plasma from the SELECT clinical trial involving over 35,000 men. The ongoing activities include processing blood on 2500 men seen on transition visits, storage of all the bloods since the study initiation in 2000, weekly generation of BSI files, ongoing communication with SELECT Statistical Center personnel, shipping samples fro the adherence sub-study, DNA extraction for roughly 400 specimens. The NSABP Repository for STAR includes ongoing storage of specimens and the maintenance of the freezers that store blood, white cells and plasma on over 19,000 women on the STAR clinical trial. The tasks involve regular checks of specimens and freezers, accession of specimens, record storage, and interaction with the NSABP.