Pathology Core

病理学随着年龄的增长呈指数级增长。因此，为了确定干预是否对衰老有影响， 长寿和健康，研究人员必须知道干预如何影响年龄相关的病理 病变此外，老年动物的病理学评估有助于研究人员确定是否发生变化， （功能、生物化学、分子等）与潜在的病理性 条件和组织学变化。这种评估还可以深入了解潜在的 干预的生物/分子机制。此外，对幼龄动物的病理分析可以 揭示遗传、药物和其他干预措施如何影响早期生命发育。因此， 在动物的整个生命周期中获得准确和全面的组织病理学评估。 在当前资助期内，南澳休克中心病理核心为50人提供了服务 研究人员（35名UTHSCSA外部人员）。所得到的数据被用于26篇出版物（包括 细胞，细胞代谢，自然细胞生物学，自然通讯，和自然医学）。迅速 随着当地和全国范围内老龄化生物学研究的扩大，我们预计， 病理学核心服务将进一步增长。 病理学核心将为研究者提供年龄相关病变的详细病理学分析， 小鼠、大鼠和其它动物模型，例如，非人类灵长类动物核心还将提供组织病理学， 特定病变和组织的形态测定、免疫组织化学和分子分析。这些包括 肿瘤病变、炎症、衰老细胞、肾小球肾炎、脑胶质增生和组织学 脂肪组织的特征。核心的具体目标是： 1.对已建立和新的癌症患者进行全面的临终和横断面病理分析， 啮齿动物模型和其他用于衰老研究的物种（包括非人类灵长类动物）。 2.进行组织/器官的免疫组织化学、分子和定量形态学分析， 啮齿动物模型和其他物种，以更好地了解与年龄相关的组织学变化。 3.继续：a）积累一个组织病理学数据和图像的综合数据库，作为一种资源 B）为新的研究提供基本的病理学信息;以及c）创建一个 组织档案作为形态学、生物化学和分子分析的资源。 4.为研究者提供组织病理学服务，准备石蜡和冷冻块， 未染色的载玻片和进行特殊染色。组织学服务还包括：a）组织制备 用于组织学/形态学实验的阵列载玻片;和B）进行激光捕获显微切割。 5.帮助教师和学员解释衰老模型中病理分析的数据 并帮助他们完成基金申请和手稿。

Pathology increases exponentially with age. Therefore, to determine if an intervention has effects on aging, longevity, and healthspan, investigators must know how the intervention affects age-related pathological lesions. Furthermore, pathological assessment of old animals helps investigators determine whether changes (functional, biochemical, molecular, etc.) are associated with, or independent of, underlying pathological conditions and histological changes. Such assessments also provide insights into potential biological/molecular mechanism(s) of the intervention. In addition, pathological analysis of young animals can reveal how genetic, pharmacological, and other interventions affect early life development. Thus, it is essential to obtain accurate and thorough histopathological assessments of animals throughout the lifespan. During the current funding period, the SA Shock Center Pathology Core performed services for 50 investigators (35 external to UTHSCSA). The resultant data were used in 26 publications (including papers in Cell, Cell Metabolism, Nature Cell Biology, Nature Communication, and Nature Medicine). With the rapid expansion of aging biology research locally and across the country, we anticipate that the demand for Pathology Core services will grow even more. The Pathology Core will provide investigators with detailed pathological analyses of age-related lesions in mice, rats, and other animal models, e.g., non-human primates. The Core will also offer histopathological, morphometric, immunohistochemical and molecular analyses of specific lesions and tissues. These include neoplastic lesions, inflammation, senescent cells, glomerulonephritis, brain gliosis, and histological characteristics of adipose tissue. The Specific Aims of the Core are: 1. To conduct comprehensive end-of-life and cross-sectional pathological analyses of established and new rodent models and other species used in aging research (including non-human primates). 2. To conduct immunohistochemical, molecular and quantitative morphometric analyses of tissues/organs of rodent models and other species to better understand age-related histological changes. 3. To continue to: a) amass a comprehensive database of histopathological data and images as a resource for the scientific community; b) provide basic pathological information for new studies; and c) create a tissue archive as a resource for morphological, biochemical and molecular analyses. 4. To provide histopathology services for investigators by preparing paraffin and frozen blocks, making unstained slides, and performing special staining. Histology services also include: a) preparation of tissue array slides for histological/morphological experiments; and b) performing laser capture microdissection. 5. To assist faculty and trainees with the interpretation of data from pathological analyses in models of aging and help them with their grant applications and manuscripts.