Geroscience Pathology and Cellular Histology

老年科学病理学和细胞组织学

• 批准号: 10561627
• 负责人: YUJI IKENO
• 金额: $ 24.25万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10561627
• 关键词: Adipocytes; Adipose tissue; Affect; Age; Aging; Animals; Archives; Biochemical; Biological; Biostatistics Core; Brain; Cell Aging; Cells; Cellular Morphology; Control Animal; Data; Data Analyses; Databases; Development; Disease; Elderly; Excision; Freezing; Frozen Sections; Future; Genetic; Geroscience; Gliosis; Glomerulonephritis; Heart; Histologic; Histology; Histopathology; Image; Immunohistochemistry; In Situ Hybridization; Infiltration; Intervention; Investigation; Islets of Langerhans; Lesion; Liver; Macrophage; Measures; Molecular; Morbidity - disease rate; Morphology; Mus; Paraffin; Paraffin Embedding; Pathologic; Pathology; Physiological; Prevalence; RNA; Rattus; Research; Research Personnel; Resource Development; Resources; Sampling; Services; Severities; Skeletal Muscle; Slide; Stains; Techniques; Testing; Tissue imaging; Tissues; Tumor Burden; age related; bone; burden of illness; cellular targeting; digital imaging; healthspan; histological stains; insight; juvenile animal; mortality; neoplastic; pharmacologic; senescence; tissue archive; trait; trend analysis

CORE D: SUMMARY Ikeno In order to determine the impact of an experimental intervention on aging, it is essential that investigators understand how an intervention alters pathological lesions that occur with age. Pathology increases exponentially with advancing age and is largely responsible for age-related morbidity and mortality. Pathological information also provides investigators with insight into the potential biological/molecular mechanism(s) of the intervention. Furthermore, pathological assessment of old animals is necessary to help investigators determine whether changes in the physiological and biochemical parameters measured are associated with or are independent of any underlying pathological conditions. In addition, pathological analysis of young animals provides investigators with information about genetic and pharmacological interventions that affect development. Thus, it is essential to obtain accurate and thorough pathological assessments of aging in both old and young animals. The Geroscience Pathology and Cellular Histology Core (GPCHC) will provide Project investigators with detailed pathological analyses of lesions that occur with age in the animals. The GPCHC will also provide investigators with cellular senescence characterization, histopathological, morphometric, and immunohistochemical analyses of specific lesions and tissues (e.g., neoplastic lesions, glomerulonephritis, gliosis in the brain, number and size of pancreatic islets, macrophage infiltration, and fat cell morphology in adipose tissue) obtained from the mice studied. In addition, the GPCHC will develop a comprehensive database of histopathological data and images of histopathology slides as a resource for: 1) trend analyses by the investigators; and 2) a tissue archive containing paraffin and frozen blocks for analysis of samples by request and for new morphological research. Furthermore, the GPCHC will provide investigators with histological support. We will prepare paraffin-embedded and frozen blocks, make unstained paraffin or frozen sections, and perform various histological staining techniques, including special staining. These data will allow investigators to evaluate the effects of genetic and pharmacological manipulations on the development of potentially fatal lesions and age-sensitive traits, and also provide information on whether the biochemical/molecular changes observed in the projects are correlated to the histopathological changes in specific tissues.

核心D：概要池野 为了确定实验干预对衰老的影响，研究人员必须 了解干预如何改变随年龄增长而发生的病理病变。病理学增加 随着年龄的增长呈指数增长，并在很大程度上造成与年龄有关的发病率和死亡率。病理 这些信息还为研究人员提供了对潜在生物学/分子机制的深入了解， 干预此外，老年动物的病理学评估是必要的，以帮助研究人员确定 所测量的生理和生化参数的变化是否与 独立于任何潜在的病理状况。此外，还对幼年动物进行病理分析 为研究人员提供了有关遗传和药物干预的信息， 发展因此，在老年人和老年人中获得准确和全面的衰老病理评估是至关重要的。 年轻的动物。老年科学病理学和细胞组织学核心（GPCHC）将提供项目 研究人员对动物随年龄增长发生的病变进行了详细的病理分析。GPCHC将 还为研究人员提供细胞衰老特征，组织病理学，形态学， 特定病变和组织的免疫组织化学分析（例如，肿瘤病变，肾小球肾炎， 脑胶质细胞增生、胰岛数量和大小、巨噬细胞浸润和脂肪细胞形态。 脂肪组织）。此外，GPCHC将建立一个全面的数据库， 组织病理学数据和组织病理学切片图像的资源，用于：1） 研究人员; 2）组织档案，其中包含石蜡和冷冻块，用于应要求分析样本 和新的形态学研究。此外，GPCHC将为研究者提供组织学检查结果。 支持.我们将准备石蜡包埋和冷冻组织块，制作未染色的石蜡或冷冻切片， 进行各种组织学染色技术，包括特殊染色。 这些数据将使研究人员能够评估遗传和药理学操作对人类免疫功能的影响。 潜在致命病变和年龄敏感性状的发展，并提供有关是否 项目中观察到的生化/分子变化与组织病理学变化相关， 特定组织