Mouse Exercise and Metabolic Phenotyping Core

小鼠运动和代谢表型核心

• 批准号: 10018900
• 负责人: DAVID H WASSERMAN
• 金额: $ 23.11万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-20 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10018900
• 关键词: Acute; Asses; Biochemical; Biological Assay; Blood specimen; Carbon Dioxide; Chronic; Data; Doctor of Philosophy; Energy Metabolism; Enzyme-Linked Immunosorbent Assay; Equipment; Exercise; Exercise Physiology; Exercise Test; Funding; Glucagon; Glycogen; Hormones; Individual; Indwelling Catheter; Infrastructure; Insulin; Laboratories; Link; Liver; Measurement; Measures; Metabolic; Modeling; Mus; Output; Pathway Analysis; Plasma; Polyacrylamide Gel Electrophoresis; Protocols documentation; Publications; Publishing; Research; Research Personnel; Role; Running; Services; Signal Pathway; Signal Transduction; Signaling Protein; Skeletal Muscle; Spectrophotometry; Speed; Testing; Tissues; Triglycerides; United States National Institutes of Health; Universities; Western Blotting; Work; cost effective; delta protein; design; energy balance; exercise capacity; exercise intensity; exercise training; experimental study; ghrelin; member; metabolic phenotype; metabolic profile; mouse model; programs; respiratory; response; treadmill; uptake

ABSTRACT The Mouse Exercise and Metabolic Phenotyping Core (Core B) will facilitate the design and execution of the exercise experiments for Program Project members. Core B leverages the facilities and infrastructure of an original NIH-funded Mouse Metabolic Phenotyping Center (MMPC, www.mmpc.org) at Vanderbilt University and the specific expertise of its Director, David Wasserman, PhD in exercise physiology. Core B has already designed and executed exercise studies that have been recently published by the Elmquist, Zigman and Williams laboratories, in addition to designing all of the exercise paradigms used to generate the respective preliminary data for the current application. (see publications section within the individual projects). Core B will provide the following Exercise Protocols and Measurements: Exercise Protocols  Incremental treadmill exercise test to assess peak O2 uptake and work capacity  Analysis of endurance capacity utilizing motorized treadmills  Perform acute high intensity interval exercise  Perform chronic exercise training Measurements  O2 uptake, CO2 output, and respiratory exchange ratio (RER) will be measured during or after exercise  Body mass and composition  Blood sampling through an indwelling catheter  Analysis of liver and skeletal muscle glycogen content  Analysis of biochemical signaling pathways  Analysis plasma analysis of insulin, ghrelin, glucagon, triglycerides (TG), and non-esterified fatty acids (NEFA)

摘要 小鼠运动和代谢表型核心（核心B）将有助于设计和执行 项目成员的运动实验。核心B利用 最初由NIH资助的范德比尔特大学小鼠代谢表型中心（MMPC，www.mmpc.org） 以及其主任大卫沃瑟曼运动生理学博士的具体专业知识。核心B已经 设计并执行了最近由Elmquist，Zigman和 威廉姆斯实验室，除了设计所有的练习范例，用于产生各自的 当前应用程序的初步数据。(see（在个别项目内的出版物部分）。 核心B将提供以下运动方案和测量： 运动方案 增量跑步机运动试验，以评估峰值O2摄取量和工作能力 使用电动跑步机的耐力分析 进行急性高强度间歇运动 进行慢性运动训练 测量 将在运动期间或运动后测量CO2摄取、CO2排出和呼吸交换率（RER 体重和成分 经留置导管采血 肝脏和骨骼肌糖原含量分析 生物化学信号通路分析 胰岛素、生长激素释放肽、胰高血糖素、甘油三酯（TG）和非酯化脂肪酸的血浆分析 （NEFA）