Vanderbilt Center for Metabolic Phenotyping in Live Models of Obesity and Diabetes

范德比尔特肥胖和糖尿病活体模型代谢表型中心

• 批准号: 10588959
• 负责人: DAVID H WASSERMAN
• 金额: $ 76.64万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10588959
• 关键词: Animals; Behavioral; Blood specimen; Body Weight; Brain region; Cannulations; Carbohydrates; Catheters; Communities; Complications of Diabetes Mellitus; Conscious; Controlled Environment; Dedications; Development; Diabetes Mellitus; Diabetic mouse; Diet; Disease; Education; Education and Outreach; Eligibility Determination; Ensure; Exercise; Faculty; Fatty acid glycerol esters; Feedback; Fiber; Fiber Optics; Foundations; Funding; Genetic; Genetic study; Glucose Clamp; Goals; Guidelines; Health; Health Services Accessibility; Hormone secretion; Hypoglycemia; Indirect Calorimetry; Infrastructure; Infusion procedures; Injections; Institution; Insulin; Islets of Langerhans Transplantation; Knowledge; Lead; Leadership; Learning; Longevity; Measurement; Medical Research; Metabolic; Metabolism; Methodology; Methods; Mission; Modeling; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; Obesity; Phenotype; Photometry; Physical activity; Physiological; Physiology; Population; Procedures; Protocols documentation; Recording of previous events; Regulation; Research; Research Personnel; Resources; Sampling; Scholarship; Scientist; Secure; Services; Standardization; Techniques; Technology; Testing; Underrepresented Populations; Venous; Viral; Work; bariatric surgery; base; biological research; cost; data management; design; drinking water; energy balance; experience; experimental study; flexibility; gene environment interaction; implantation; improved; innovation; meetings; metabolic abnormality assessment; metabolic phenotype; mouse model; neuroregulation; novel; optical fiber; optogenetics; outreach; outreach program; oxidation; pharmacologic; programs; respiratory; response; skills; solute; success; surgical service; targeted delivery; telemetering; tool; treadmill; welfare

The Vanderbilt Center for Metabolic Phenotyping in Live Models of Obesity and Diabetes (VMPMOD) is built on the strong Vanderbilt history of serving as a resource to study conscious, unstressed mice. During its 20-year lifespan the Vanderbilt Mouse Metabolic Phenotyping Center advanced research in diabetes, obesity, and metabolism by providing the national scientific community with innovative and high-quality phenotyping services to study genetic mouse models. Quality services and access to experienced faculty has created a large demand from "outside investigators" to use the Center. VMPMOD consists of three cores that are dedicated to serving outside investigators. The Administrative Core provides scientific, financial, and administrative leadership. This Core oversees service requests and data management. It is responsible for educational programs and the Vanderbilt Vibrant Program that provides access and support for underrepresented populations and early-stage investigators. The Animal Health and Welfare Core evaluate mices submitted to VMPMOD, oversees mouse health and welfare, and ensures compliance with regulatory bodies and VMPMOD guidelines. The Mouse Metabolic Physiology Core provides a range of surgical services for mice and flexible experimental services performed under customizable environment-controlled conditions. These experimental services are conducted by the Metabolic Regulation Subcore (MRSC) and the Body Weight Regulation Subcore (BWRSC). MRSC uses flexible platforms to study insulin action, hormone secretion, hypoglycemic regulation, exercise metabolism, and metabolic flux analysis with and without simultaneous measurements of vO2, vCO2, respiratory exchange ratio, carbohydrate oxidation, and fat oxidation. BWRSC utilizes techniques that allow for granular measurements of the components of energy balance under a wide range of conditions. Chemogenetics, optogenetics, and fiber photometry are applied in the MRSC and BWRSC to study neural control mechanisms in concurrence with studies of metabolism and energy balance. The proposed VMPMOD has made significant technical developments that create new opportunities for advances in our understanding of diabetes, obesity, and metabolism. The Vanderbilt mouse program has been successful because it is comprised of a faculty willing to develop and make technology that is part of their research lifeline available to the scientific community and because of staff that are so skilled and committed that scientists are willing to entrust their mice, their research lifelines, with them. The same faculty and staff that has made the program successful in the past will continue to lead VMPMOD.

范德比尔特肥胖和糖尿病活体模型代谢表型研究中心（VMPMOD）建立在范德比尔特作为研究清醒、无压力小鼠的资源的强大历史基础上。在其20年的生命周期中，范德比尔特小鼠代谢表型中心通过为国家科学界提供创新和高质量的表型服务来研究遗传小鼠模型，从而推进了糖尿病，肥胖和代谢方面的研究。优质的服务和有经验的教师创造了大量的需求，从“外部调查”使用中心。VMPMOD由三个核心组成，专门为外部调查员提供服务。行政核心提供科学，财务和行政领导。该核心负责监督服务请求和数据管理。它负责教育计划和范德比尔特充满活力的计划，为代表性不足的人群和早期研究人员提供访问和支持。动物健康和福利中心评估提交给VMPMOD的小鼠，监督小鼠健康和福利，并确保遵守监管机构和VMPMOD指南。Mouse Metabolic Physiology Core为小鼠提供一系列手术服务，并在可定制的环境控制条件下进行灵活的实验服务。这些实验服务由代谢调节子核心（MRSC）和体重调节子核心（BWRSC）进行。MRSC使用灵活的平台研究胰岛素作用、激素分泌、降血糖调节、运动代谢和代谢通量分析，同时测量和不测量vO2、vCO2、呼吸交换率、碳水化合物氧化和脂肪氧化。BWRSC利用技术，允许在广泛的条件下对能量平衡的组成部分进行粒度测量。在MRSC和BWRSC中应用化学遗传学、光遗传学和纤维光度学来研究神经控制机制，同时研究代谢和能量平衡。拟议的VMPMOD已经取得了重大的技术发展，为我们对糖尿病，肥胖和代谢的理解的进步创造了新的机会。范德比尔特的小鼠项目之所以成功，是因为它由一群愿意开发和制造技术的教师组成，这些技术是他们研究生命线的一部分，可供科学界使用，也是因为工作人员技术娴熟、敬业，科学家们愿意将他们的小鼠、他们的研究生命线托付给他们。同样的教师和工作人员，使该计划在过去取得成功将继续领导VMPMOD。