Mouse Exercise and Metabolic Phenotyping Core
小鼠运动和代谢表型核心
基本信息
- 批准号:10242069
- 负责人:
- 金额:$ 22.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-20 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AcuteBiochemicalBiological AssayBlood specimenCarbon DioxideChronicDataDoctor of PhilosophyEnergy MetabolismEnzyme-Linked Immunosorbent AssayEquipmentExerciseExercise PhysiologyExercise TestFundingGlucagonGlycogenHormonesIndividualIndwelling CatheterInfrastructureInsulinLaboratoriesLinkLiverMeasurementMeasuresMetabolicModelingMusOutputPathway AnalysisPlasmaPolyacrylamide Gel ElectrophoresisProtocols documentationPublicationsPublishingResearchResearch PersonnelRoleRunningServicesSignal PathwaySignal TransductionSignaling ProteinSkeletal MuscleSpectrophotometrySpeedTestingTissuesTriglyceridesUnited States National Institutes of HealthUniversitiesWestern BlottingWorkcost effectivedelta proteindesignenergy balanceexercise capacityexercise intensityexercise trainingexperimental studyghrelinmembermetabolic phenotypemouse modelprogramsrespiratoryresponsetreadmilluptake
项目摘要
ABSTRACT
The Mouse Exercise and Metabolic Phenotyping Core (Core B) will facilitate the design and execution of
the exercise experiments for Program Project members. Core B leverages the facilities and infrastructure of an
original NIH-funded Mouse Metabolic Phenotyping Center (MMPC, www.mmpc.org) at Vanderbilt University
and the specific expertise of its Director, David Wasserman, PhD in exercise physiology. Core B has already
designed and executed exercise studies that have been recently published by the Elmquist, Zigman and
Williams laboratories, in addition to designing all of the exercise paradigms used to generate the respective
preliminary data for the current application. (see publications section within the individual projects).
Core B will provide the following Exercise Protocols and Measurements:
Exercise Protocols
Incremental treadmill exercise test to assess peak O2 uptake and work capacity
Analysis of endurance capacity utilizing motorized treadmills
Perform acute high intensity interval exercise
Perform chronic exercise training
Measurements
O2 uptake, CO2 output, and respiratory exchange ratio (RER) will be measured during or after exercise
Body mass and composition
Blood sampling through an indwelling catheter
Analysis of liver and skeletal muscle glycogen content
Analysis of biochemical signaling pathways
Analysis plasma analysis of insulin, ghrelin, glucagon, triglycerides (TG), and non-esterified fatty acids
(NEFA)
摘要
小鼠运动和代谢表型核心(核心B)将促进以下内容的设计和执行
项目成员的运动实验。核心B利用
最初由NIH资助的范德比尔特大学小鼠代谢表型中心(MMPC,www.mmpc.org)
以及其主任大卫沃瑟曼运动生理学博士的具体专业知识。核心B已经
设计并执行了最近由Elmquist,Zigman和
威廉姆斯实验室,除了设计所有的练习范例,用于产生各自的
当前应用程序的初步数据。(see(在个别项目内的出版物部分)。
核心B将提供以下运动方案和测量:
运动方案
增量跑步机运动试验,以评估峰值O2摄取量和工作能力
使用电动跑步机的耐力分析
进行急性高强度间歇运动
进行慢性运动训练
测量
将在运动期间或运动后测量CO2摄取、CO2排出和呼吸交换率(RER
体重和成分
经留置导管采血
肝脏和骨骼肌糖原含量分析
生物化学信号通路分析
胰岛素、生长激素释放肽、胰高血糖素、甘油三酯(TG)和非酯化脂肪酸的血浆分析
(NEFA)
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DAVID H WASSERMAN其他文献
DAVID H WASSERMAN的其他文献
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{{ truncateString('DAVID H WASSERMAN', 18)}}的其他基金
Vanderbilt Center for Metabolic Phenotyping in Live Models of Obesity and Diabetes
范德比尔特肥胖和糖尿病活体模型代谢表型中心
- 批准号:
10588959 - 财政年份:2023
- 资助金额:
$ 22.82万 - 项目类别:
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