Mouse Exercise and Metabolic Phenotyping Core

摘要 老鼠练习和代谢表型核心(核心B)将促进设计和执行 针对计划项目成员的练习实验。核心B利用 范德比尔特大学由美国国立卫生研究院资助的小鼠代谢表型鉴定中心(MMPC，www.mmpc.org) 以及其主任大卫·沃瑟曼在运动生理学方面的博士学位。核心B已经 设计和执行运动研究，最近由Elmquist，Zigman和 威廉姆斯实验室，除了设计用于产生各自的 当前应用程序的初步数据。(见个别项目内的出版物部分)。 核心B将提供以下演练方案和测量： 锻炼计划 递增平板运动试验评估峰值氧摄取和工作能力 电动跑步机耐力的分析 进行急性高强度间歇运动 进行慢性运动训练 测量结果 在运动中或运动后，将测量O2摄取量、二氧化碳排出量和呼吸交换率 身体质量和成分 留置导尿管采集血样 肝脏和骨骼肌糖原含量的分析 生化信号通路的分析 分析血浆中胰岛素、Ghrelin、胰高血糖素、甘油三酯和非酯化脂肪酸 (NEFA)

ABSTRACT The Mouse Exercise and Metabolic Phenotyping Core (Core B) will facilitate the design and execution of the exercise experiments for Program Project members. Core B leverages the facilities and infrastructure of an original NIH-funded Mouse Metabolic Phenotyping Center (MMPC, www.mmpc.org) at Vanderbilt University and the specific expertise of its Director, David Wasserman, PhD in exercise physiology. Core B has already designed and executed exercise studies that have been recently published by the Elmquist, Zigman and Williams laboratories, in addition to designing all of the exercise paradigms used to generate the respective preliminary data for the current application. (see publications section within the individual projects). Core B will provide the following Exercise Protocols and Measurements: Exercise Protocols  Incremental treadmill exercise test to assess peak O2 uptake and work capacity  Analysis of endurance capacity utilizing motorized treadmills  Perform acute high intensity interval exercise  Perform chronic exercise training Measurements  O2 uptake, CO2 output, and respiratory exchange ratio (RER) will be measured during or after exercise  Body mass and composition  Blood sampling through an indwelling catheter  Analysis of liver and skeletal muscle glycogen content  Analysis of biochemical signaling pathways  Analysis plasma analysis of insulin, ghrelin, glucagon, triglycerides (TG), and non-esterified fatty acids (NEFA)