Role of Piezo2 and Nav channels in rapidly-adapting neuronal mechanoreceptors

Piezo2 和 Nav 通道在快速适应神经元机械感受器中的作用

• 批准号: 10063569
• 负责人: Sviatoslav Bagriantsev
• 金额: $ 36.64万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-01 至 2021-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10063569
• 关键词: Action Potentials; Acute; Afferent Neurons; Animal Model; Axon; Cardiac; Cations; Complex; Data; Development; Ducks; Embryo; Environment; Evolution; Fingers; Foundations; Generations; Goals; Hand; Hearing; Ion Channel Gating; Knowledge; Lentivirus; Life; Light; Mechanical Stimulation; Mechanics; Mechanoreceptors; Mediating; Meissners Corpuscle; Minor; Modality; Modeling; Molecular; Neurons; Nociceptors; Organ; Pacinian Corpuscles; Pain; Pathway interactions; Perception; Peripheral; Pharmacology; Physiological; Physiology; Piezo 2 ion channel; Primates; Process; Rodent; Rodent Model; Role; Sensory; Sensory Ganglia; Sensory Physiology; Shapes; Skin; Small Interfering RNA; Smell Perception; Sodium; Sodium Channel; Stimulus; Stretching; Structure of trigeminal ganglion; Surface; Tactile; Taste Perception; Testing; Texture; Thermoreceptors; Touch sensation; Trigeminal System; Vertebrates; Vision; Work; base; density; detector; experimental study; knock-down; mechanical force; mechanotransduction; millimeter; nervous system disorder; novel; receptor; relating to nervous system; response; small hairpin RNA; transmission process; vibration; voltage

Of all the sensory modalities possessed by vertebrates, the ability to sense mechanical force remains the least well understood at the cellular and molecular level. In the glabrous skin, touch is sensed by Meissner and Pacinian corpuscles – the detectors of fine objects and minute skin deformations. The corpuscles are innervated by rapidly-adapting neuronal mechanoreceptors, which can directly convert touch into excitation, by an unknown mechanism. Here, we aim to elucidate this mechanism by studying the mechanosensitivity in rapidly-adapting mechanoreceptors, using the trigeminal system of the duck embryo as a model. Duck trigeminal ganglia contain a large proportion of rapidly-adapting mechanoreceptors, which innervate the numerous Meissner- and Pacinian-like corpuscles in the glabrous skin of the bill. Using this model, we aim to determine the role of stretch-sensitive cationic and voltage-sensitive sodium channels in the three principal processes of touch sensitivity: (1) the conversion of touch into excitatory current; (2) the generation of action potential, and (3) propagation of the rapidly-adapting afferent message along the axon. Our studies will reveal cellular and molecular principles underlying the sense of touch in rapidly-adapting neuronal mechanoreceptors.

在脊椎动物拥有的所有感觉方式中，感觉机械力的能力仍然存在。 在细胞和分子水平上了解最少。在无毛的皮肤中，触觉是通过 迈斯纳和Pacinian小体-探测器的细微物体和微小的皮肤变形。的 小体由快速适应的神经元机械感受器支配，其可以直接将 通过一种未知的机制进入兴奋状态。在这里，我们的目标是阐明这一机制， 研究快速适应机械感受器的机械敏感性，使用三叉神经系统， 鸭胚胎作为模型。鸭三叉神经节含有较大比例的快速适应神经元 机械感受器，它支配的众多迈斯纳和帕西尼样小体在 无毛的喙皮。使用这个模型，我们的目标是确定拉伸敏感的阳离子和 电压敏感性钠通道在触摸敏感性的三个主要过程中：（1） 触摸转换为兴奋电流;（2）动作电位的产生，以及（3） 沿着轴突快速适应传入信息。我们的研究将揭示细胞和分子 在快速适应的神经元机械感受器中的触觉的基本原理。