The role of the circadian clock in the behavior of the malaria mosquito Anopheles coluzzii

生物钟在疟疾蚊子按蚊行为中的作用

• 批准号: 10083702
• 负责人: Zach N. Adelman
• 金额: $ 18.94万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-01-09 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10083702
• 关键词: Adult; Aedes; Affect; Africa South of the Sahara; Animal Model; Anopheles Genus; Behavior; Behavioral; Biological Clocks; Biology; Blood; CRISPR/Cas technology; Candidate Disease Gene; Culicidae; Development; Drosophila genus; Feedback; Female; Gene Expression; Generations; Genes; Genetic Transcription; Hour; Human; Immunity; Insecticide Resistance; Insemination; Knock-out; Libido; Link; Malaria; Mediating; Membrane; Motor Activity; Odors; Organism; Oviposition; Partner in relationship; Pattern; Periodicity; Proxy; Pupa; Quantitative Reverse Transcriptase PCR; Regulation; Reporting; Research; Role; Series; Smell Perception; Speed; Testing; Time; Work; circadian; circadian pacemaker; egg; feeding; hatching; malaria mosquito; male; mating behavior; mutant; preference; programs; response; success; sugar; vector; vector competence

Project Summary Anopheles coluzzii is one of most important vectors in sub-Saharan Africa. One reason for this is its high preference for human hosts. Like all organisms, An. coluzzii has an internal clock that controls much of its biology and behavior. For example, An. coluzzii is active in the evening and at night, and mating, host seeking, sugar feeding and oviposition takes places during this time. For example, spontaneous flight activity of this mosquito is all but absent during the day but shows two strong peaks of activity during the night: one in the early evening and one towards the end of the night. Previous work has also shown that the expression of many genes is controlled by the internal clock. This includes genes involved in olfaction, immunity and insecticide resistance. The internal clock consists of several genes, including the genes clock and cycle, that are expressed rhythmically. Together with other genes they form a feedback loop that cycles approximately every 24 hours. In Aedes mosquitoes, which are active at night, shutting down this internal clock by disabling the cycle gene results in much reduced host seeking, longer development times and reduced survival. Because the internal clock affects day-time and night-time mosquitoes differently, the results from Aedes cannot be extrapolated to Anopheles. Therefore, to examine to what extent shutting down the internal clock affects the behavior, development and survival of An. coluzzii, we will use CRISPR-Cas9 to disable the clock or cycle gene. We will evaluate how this affects spontaneous flight activity, host seeking, mating success, as well as developmental rate and survival. This will tell us to what extent this mosquito’s behavior is controlled by the internal clock vs external conditions (e.g light/dark). It may also provide a way to modify mosquito behavior, e.g. by reducing host seeking, to reduce its ability to transmit malaria.

项目摘要 Anopheles Coluzzii是撒哈拉以南非洲最重要的载体之一。原因之一是它的高 偏爱人类寄主。像所有生物一样，一个。 Coluzzii具有控制大部分的内部时钟 生物学和行为。例如，一个。 Coluzzii在晚上和晚上都活跃，并交配，主持人 在此期间，寻求，进食和产卵。例如，赞助飞行活动 白天几乎不存在这个蚊子 在傍晚，直到深夜。以前的工作还表明了表达 许多基因都由内部时钟控制。这包括涉及嗅觉，免疫力和 杀虫剂抗性。内部时钟由几个基因组成，包括基因时钟和周期， 在逻辑上表示。与其他基因一起，它们形成一个反馈回路，该回路大致循环 每24小时一次。在夜间活跃的艾德斯蚊子中，通过禁用该内部时钟 循环基因导致宿主的寻求大大减少，发育时间更长和生存率降低。 由于内部时钟会影响白天和夜间蚊子，因此伊德斯的结果 不能外推到蚊子。因此，要检查在多大程度上关闭内部时钟 影响an的行为，发展和生存。 coluzzii，我们将使用crispr-cas9禁用时钟 或循环基因。我们将评估这如何影响赞助的飞行活动，寻求主机，交配成功，作为 以及发展速度和生存。这将告诉我们这种蚊子的行为在多大程度上受到控制 通过内部时钟与外部条件（例如光/黑暗）。它也可能提供一种修改蚊子的方法 行为，例如通过减少宿主寻求，降低其传播疟疾的能力。