Functional analysis of the FORKED and FORKED-LIKE proteins and their role in the environmental control of leaf vein pattern

FORKED和FORKED-LIKE蛋白的功能分析及其在叶脉模式环境控制中的作用

• 批准号: RGPIN-2017-04381
• 负责人: Schultz, Elizabeth
• 金额: $ 2.04万
• 依托单位: University of Lethbridge
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744828
• 关键词: Functional; analysis; FORKED; LIKE; proteins

Plants are exposed to a constantly changing environment from which they cannot escape. Instead, they alter development to produce a form better adapted to the new environment. The hormone auxin controls many important events in plant development such as defining where leaves form, where shoots and root branch and turning roots towards gravity. Auxin is directionally transported from cell to cell dependent upon the polar localization of PINFORMED (PIN) transport proteins to a particular side of the cell membrane. Many of the environmental responses of plant growth are achieved by changing PIN localization and hence cell polarity, tissue growth and organization. I am interested in the formation and organization of leaf veins, which also depends on polar localization of PIN within cells that will become veins. My group discovered the FORKED1 (FKD1) gene, that is important for localizing PIN within these cells. If the gene is mutated, PIN is never localized to the top side of cells, and rather than meeting to form the normal closed loops, the veins form an open, “forking” vein pattern. We found that FKD1 works in the secretory pathway, taking the PIN protein to the plasma membrane. I propose to use FKD1 to study other factors that direct proteins to the top side of cells, a previously proposed pathway that is little understood. FKD1 is in a gene family unique to plants and found in all plant genomes. The gene first appears in non-vascular liverworts, indicating that its function predates veins. A mutant that knocks out four members of the Arabidopsis gene family has short roots that do not respond to gravity and misshapen, lumpy leaves. Microscopic observation reveals that the bumps and hollows contain patches of randomly oriented cells. Cells in leaves are normally polarized with their long ends parallel to the midvein. While botanists have suggested that auxin might polarize the cells within the leaf, no one has been able to test the idea experimentally. The mutants provide a unique tool to explore the mechanism by which leaf cells are polarized. Vein pattern changes in response to the environment. For example, drought conditions cause a higher vein density with more joints, an adaptation that helps plants maintain photosynthesis. Interestingly, members of the gene family have sequences that suggest regulation by drought and the stress hormone ABA, and my students have shown that FKD1 responds to ABA. As well, mutation to FKD1 or other gene family members eliminates the vein pattern response to drought. Therefore, I propose that the gene family allows vein pattern adaptation to stress, through its regulation by stress-signaling pathways including ABA, and have designed experiments to test this idea. By revealing novel processes that achieve PIN localization and cell polarity and defining how the environment influences these processes, my research will allow manipulation of plant form better suited to the environment.

植物暴露在一个不断变化的环境中，它们无法逃脱。 相反，它们改变发育，以产生更好地适应新环境的形式。生长素激素控制着植物发育中的许多重要事件，例如确定叶子的形成位置，芽和根的分支的位置以及使根转向重力。 生长素在细胞间的定向转运依赖于PINFORMED（PIN）转运蛋白在细胞膜特定一侧的极性定位。植物生长的许多环境响应是通过改变PIN定位并因此改变细胞极性、组织生长和组织来实现的。 我对叶脉的形成和组织感兴趣，这也取决于PIN在细胞内的极性定位，这些细胞将成为叶脉。 我的团队发现了FORKED 1（FKD 1）基因，这对定位这些细胞内的PIN很重要。如果基因发生突变，PIN永远不会定位于细胞的顶部，而不是相遇形成正常的闭合环，静脉形成开放的“分叉”静脉模式。我们发现FKD 1在分泌途径中起作用，将PIN蛋白带到质膜。 我建议使用FKD 1来研究将蛋白质引导到细胞顶部的其他因子，这是一种以前提出的途径，但人们对此知之甚少。 FKD 1是植物特有的基因家族，存在于所有植物基因组中。 该基因首先出现在非维管苔类中，表明其功能早于静脉。 一个敲除拟南芥基因家族四个成员的突变体具有对重力没有反应的短根和畸形的淡黄色叶子。 显微镜观察显示，凹凸不平的地方含有随机取向的细胞。叶细胞的长端通常与中脉平行。 虽然植物学家提出生长素可能会使叶子内的细胞生长，但没有人能够通过实验来验证这个想法。 突变体提供了一个独特的工具来探索叶细胞极化的机制。静脉模式会随着环境的变化而变化。 例如，干旱条件导致更高的静脉密度和更多的关节，这是一种帮助植物维持光合作用的适应。 有趣的是，该基因家族的成员具有表明受干旱和应激激素阿坝调节的序列，我的学生已经证明FKD 1对阿坝有反应。 同样，FKD 1或其他基因家族成员的突变消除了对干旱的静脉模式反应。 因此，我建议，基因家族允许静脉图案适应压力，通过其调节压力信号通路，包括阿坝，并设计了实验来测试这一想法。 通过揭示实现PIN定位和细胞极性的新过程，并定义环境如何影响这些过程，我的研究将允许操纵更适合环境的植物形态。