BioCARS: Structural Dynamics and Biological Mechanisms
BioCARS:结构动力学和生物机制
基本信息
- 批准号:10093063
- 负责人:
- 金额:$ 128.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-02-01 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAlgorithmsAreaAutomobile DrivingBiologicalBiological ProcessCatalysisCharacteristicsChargeCollaborationsCommunitiesComplexCoupledCrystallizationCrystallographyDataData AnalysesData SetDevelopmentDevicesDiseaseEducational workshopEngineeringEnvironmentEnzymesExperimental DesignsGeneral PopulationGoalsImageImprove AccessKnowledgeLaboratoriesMeasurementMethodsMicrofluidicsModernizationMolecularMolecular ConformationMotionPharmacologyPhotonsPhysiologicalPrinted MediaProcessProteinsRegulationResolutionRoentgen RaysSamplingScienceServicesSignal TransductionSoftware ToolsSourceStructureSynchrotronsSystemTechnologyTemperatureTherapeuticTimeTrainingUpdateWorkX ray diffraction analysisX-Ray Crystallographybasechemical reactioncomputerized data processingdesigndetectorelectric fieldexperimental studyhigh schoolinterestmacromoleculemeetingsnew technologynext generationnovelnovel strategiesopen sourceposterspractical applicationprospectiveresearch and developmentresearch facilitysymposiumtechnology developmenttechnology research and developmenttime usetooltransmission processundergraduate student
项目摘要
Abstract
A major goal is biomedical science is to move beyond static images of proteins and other biological
macromolecules to the internal dynamics underlying their function. This level of study is necessary to
understand how these molecules work, to engineer new functions, and to rationally develop therapeutics. In
this application, we propose two technological research and development projects that take advantage of the
special characteristics of the BioCARS national synchrotron facility to address these goals. In the first, TR&D 1,
we describe time-resolved serial micro-crystallography (TR-SMX) coupled with initiation of chemical reactions
within molecules. This approach is enabled by the high intensity and tight focusing of X-rays at BioCARS, the
availability of a large area, fast detectors, and the use of novel crystal injectors and microfluidic mixers. TR-
SMX enables users to observe the dynamics of molecules as they execute their biological function at
physiological temperatures. In the second, TR&D 2, we describe electric field-stimulated X-ray crystallography
(EFX), a new method visualizing conformational changes within proteins and other biological macromolecules.
This method uses external electric fields to induce global, subtle motions of atoms within proteins, with readout
using time-resolved X-ray diffraction. Initial work demonstrates the practical application of EFX, and confirms
the ability to globally excite motions throughout a protein molecule, including those of biological relevance.
Since charges and dipoles are universally present in macromolecules, EFX represents, in principle, a general
approach for intramolecular dynamics. We describe several user-based driving biomedical projects and
collaborative and service projects that cover a wide range of important problems and push both technologies.
User training and active approaches to dissemination to both expert and non-expert, wider audiences will
enable broad use of the new technologies by the scientific community.
摘要
项目成果
期刊论文数量(35)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Microsecond dynamics control the HIV-1 Envelope conformation.
- DOI:10.1126/sciadv.adj0396
- 发表时间:2024-02-02
- 期刊:
- 影响因子:13.6
- 作者:Bennett, Ashley L.;Edwards, Robert;Kosheleva, Irina;Saunders, Carrie;Bililign, Yishak;Williams, Ashliegh;Bubphamala, Pimthada;Manosouri, Katayoun;Anasti, Kara;Saunders, Kevin O.;Alam, S. Munir;Haynes, Barton F.;Acharya, Priyamvada;Henderson, Rory
- 通讯作者:Henderson, Rory
Microbial production of megadalton titin yields fibers with advantageous mechanical properties.
- DOI:10.1038/s41467-021-25360-6
- 发表时间:2021-08-30
- 期刊:
- 影响因子:16.6
- 作者:Bowen CH;Sargent CJ;Wang A;Zhu Y;Chang X;Li J;Mu X;Galazka JM;Jun YS;Keten S;Zhang F
- 通讯作者:Zhang F
Resolving conformational changes that mediate a two-step catalytic mechanism in a model enzyme.
解决模型酶中介导两步催化机制的构象变化。
- DOI:10.1101/2023.06.02.543507
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Greisman,JackB;Dalton,KevinM;Brookner,DennisE;Klureza,MargaretA;Sheehan,CandiceJ;Kim,In-Sik;Henning,RobertW;Russi,Silvia;Hekstra,DoekeR
- 通讯作者:Hekstra,DoekeR
Axial vs equatorial: Capturing the intramolecular charge transfer state geometry in conformational polymorphic crystals of a donor-bridge-acceptor dyad in nanosecond-time-scale.
- DOI:10.1063/5.0134792
- 发表时间:2023-02-07
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Structural dynamics of a thermally silent triiron(II) spin crossover defect grid complex.
- DOI:10.1039/d3dt02067c
- 发表时间:2023-09-13
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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JOHN Keith MOFFAT其他文献
JOHN Keith MOFFAT的其他文献
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{{ truncateString('JOHN Keith MOFFAT', 18)}}的其他基金
Structures, Dynamics and Signaling Mechanisms of Bacteriophytochromes
细菌光敏色素的结构、动力学和信号机制
- 批准号:
8842642 - 财政年份:2014
- 资助金额:
$ 128.97万 - 项目类别:
Structures, Dynamics and Signaling Mechanisms of Bacteriophytochromes
细菌光敏色素的结构、动力学和信号机制
- 批准号:
8672967 - 财政年份:2014
- 资助金额:
$ 128.97万 - 项目类别:
BioCARS: A Synchrotron Structure Biology Resource
BioCARS:同步加速器结构生物学资源
- 批准号:
8735172 - 财政年份:2013
- 资助金额:
$ 128.97万 - 项目类别:
BioCARS: A Synchrotron Structure Biology Resource
BioCARS:同步加速器结构生物学资源
- 批准号:
8727171 - 财政年份:2013
- 资助金额:
$ 128.97万 - 项目类别:
STUDYING NATURAL AND DESIGNED RED-LIGHT PHOTORECEPTORS BY STATIC AND DYNAMIC
通过静态和动态研究天然和设计的红光感光器
- 批准号:
8363682 - 财政年份:2011
- 资助金额:
$ 128.97万 - 项目类别:
High Speed Detector for Time-Resolved Research at BioCARS
BioCARS 用于时间分辨研究的高速探测器
- 批准号:
7836715 - 财政年份:2011
- 资助金额:
$ 128.97万 - 项目类别:
EXPLORING LIGHT-SENSING AND SIGNALING MECHANISMS OF BACTERIOPHYTOCHROMES BY C
利用 C 探索细菌植物色素的光传感和信号传导机制
- 批准号:
8171982 - 财政年份:2010
- 资助金额:
$ 128.97万 - 项目类别:
EXPLORING LIGHT-SENSING AND SIGNALING MECHANISMS OF BACTERIOPHYTOCHROMES BY C
利用 C 探索细菌植物色素的光传感和信号传导机制
- 批准号:
7956811 - 财政年份:2009
- 资助金额:
$ 128.97万 - 项目类别:
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