STUDYING NATURAL AND DESIGNED RED-LIGHT PHOTORECEPTORS BY STATIC AND DYNAMIC
通过静态和动态研究天然和设计的红光感光器
基本信息
- 批准号:8363682
- 负责人:
- 金额:$ 6.69万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-08-01 至 2012-07-31
- 项目状态:已结题
- 来源:
- 关键词:BerylliumBindingBiologicalC-terminalCircadian RhythmsCollaborationsCrystallographyDevelopmentEventFamilyFundingGrantIon ChannelLengthLifeLightMediatingMolecularN-terminalNational Center for Research ResourcesOrganismPerceptionPhosphotransferasesPhotophobiaPhotoreceptorsPhysiological ProcessesPhytochromePrincipal InvestigatorProteinsPseudomonas aeruginosaReactionResearchResearch InfrastructureResourcesScanningScientistSignal TransductionSourceStructureSystemTemperatureTimeUnited States National Institutes of Healthbasebeamlinechromophorecostdesigninsightplant fungiprotein-histidine kinaseresponsestructural biology
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Light is a fundamental signal that regulates important physiological processes such as development and circadian rhythm in living organisms. Phytochromes form a major family of photoreceptors responsible for red light perception in plants fungi and bacteria1. They undergo reversible photoconversion between red-absorbing (Pr) and far-red-absorbing (Pfr) states thereby ultimately converting a light signal into a distinct biological signal that mediates subsequent cellular responses. Our research aims to understand the molecular mechanisms of Pr/Pfr photoconversion and signal transduction in phytochromes using representative bacteriophytochromes (BphP) from P. aeruginosa and R. palustris. BphPs consist of three N-terminal photosensory domains (PAS GAF and PHY) and a C-terminal histidine kinase (HK) effector domain in which the HK activity is regulated by light signals detected by the photosensory core domains (PCD). During previous proposal periods we have determined several crystal structures of PCD in the Pr and Pfr states respectively. We have also captured three early reaction intermediates by initiating and following the Pfr-to-Pr photoreaction in photoactive PaBphP crystals using temperature-scanning cryo-crystallography. Taken together these crystal structures of reactant product and intermediate states provide structural insight into initial molecular events of BphPs upon sensing red-light and subsequent conformational changes in the chromophore and adjacent protein matrix. During the next period (1) we plan to extend our crystallographic studies to full-length (FL) BphPs which include a C-terminal hisitidine kinase (HK) effector domain. We aim to identify tertiary and quaternary structural elements that are responsible for transmitting light-induced local conformational changes in the chromophore-binding pocket to the remote HK to generate a biological signal. (2) We will further explore time-resolved experimental strategies based on both Laue and monochromatic diffraction to study reaction intermediates on various time-scales (from ps to ms) in collaboration with beamline scientists at BioCARS. (3) We will carry out crystallographic studies on artificial photoreceptors if suitable projects/crystals arise in couple with our ongoing protein design efforts to convey light-sensitivity to otherwise light-inert systems such as ion channels.
这个子项目是许多利用资源的研究子项目之一
由NIH/NCRR资助的中心拨款提供。子项目的主要支持
而子项目的主要调查员可能是由其他来源提供的,
包括其它NIH来源。 列出的子项目总成本可能
代表子项目使用的中心基础设施的估计数量,
而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。
光是调节生物体发育和昼夜节律等重要生理过程的基本信号。光敏色素是植物、真菌和细菌中负责红光感知的光感受器的一个主要家族。它们在红色吸收(Pr)和远红色吸收(Pfr)状态之间进行可逆的光转换,从而最终将光信号转换为介导随后细胞反应的独特生物信号。本研究以铜绿假单胞菌和红球藻的代表性细菌光敏色素(BphP)为研究对象,探讨Pr/Pfr光转化和信号转导的分子机制。沼泽BphPs由三个N末端光敏结构域(PAS GAF和PHY)和一个C末端组氨酸激酶(HK)效应结构域组成,其中HK活性受光敏核心结构域(PCD)检测到的光信号调节。在以前的建议期间,我们已经确定了几个晶体结构的PCD在Pr和Pfr状态分别。我们还捕获了三个早期的反应中间体,通过启动和以下的PFR到Pr的光反应,在光敏PaBphP晶体使用温度扫描cryo-crystallography。总之,这些反应产物和中间状态的晶体结构提供了结构洞察BphPs的初始分子事件后,感测红光和随后的构象变化的发色团和相邻的蛋白质基质。在下一阶段(1),我们计划将我们的晶体学研究扩展到全长(FL)BphPs,其中包括C-末端组氨酸激酶(HK)效应结构域。我们的目标是确定三级和四级结构元件,负责传输光诱导的局部构象变化的发色团结合口袋的远程HK产生的生物信号。 (2)我们将进一步探索基于劳厄和单色衍射的时间分辨实验策略,与BioCARS的光束线科学家合作,研究各种时间尺度(从ps到ms)的反应中间体。(3)如果合适的项目/晶体与我们正在进行的蛋白质设计工作相结合,我们将对人工光感受器进行晶体学研究,以将光敏性传递给其他光惰性系统,如离子通道。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JOHN Keith MOFFAT其他文献
JOHN Keith MOFFAT的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JOHN Keith MOFFAT', 18)}}的其他基金
BioCARS: Structural Dynamics and Biological Mechanisms
BioCARS:结构动力学和生物机制
- 批准号:
10093063 - 财政年份:2019
- 资助金额:
$ 6.69万 - 项目类别:
Structures, Dynamics and Signaling Mechanisms of Bacteriophytochromes
细菌光敏色素的结构、动力学和信号机制
- 批准号:
8842642 - 财政年份:2014
- 资助金额:
$ 6.69万 - 项目类别:
Structures, Dynamics and Signaling Mechanisms of Bacteriophytochromes
细菌光敏色素的结构、动力学和信号机制
- 批准号:
8672967 - 财政年份:2014
- 资助金额:
$ 6.69万 - 项目类别:
BioCARS: A Synchrotron Structure Biology Resource
BioCARS:同步加速器结构生物学资源
- 批准号:
8735172 - 财政年份:2013
- 资助金额:
$ 6.69万 - 项目类别:
BioCARS: A Synchrotron Structure Biology Resource
BioCARS:同步加速器结构生物学资源
- 批准号:
8727171 - 财政年份:2013
- 资助金额:
$ 6.69万 - 项目类别:
High Speed Detector for Time-Resolved Research at BioCARS
BioCARS 用于时间分辨研究的高速探测器
- 批准号:
7836715 - 财政年份:2011
- 资助金额:
$ 6.69万 - 项目类别:
EXPLORING LIGHT-SENSING AND SIGNALING MECHANISMS OF BACTERIOPHYTOCHROMES BY C
利用 C 探索细菌植物色素的光传感和信号传导机制
- 批准号:
8171982 - 财政年份:2010
- 资助金额:
$ 6.69万 - 项目类别:
EXPLORING LIGHT-SENSING AND SIGNALING MECHANISMS OF BACTERIOPHYTOCHROMES BY C
利用 C 探索细菌植物色素的光传感和信号传导机制
- 批准号:
7956811 - 财政年份:2009
- 资助金额:
$ 6.69万 - 项目类别:
相似国自然基金
帽结合蛋白(cap binding protein)调控乙烯信号转导的分子机制
- 批准号:32170319
- 批准年份:2021
- 资助金额:58.00 万元
- 项目类别:面上项目
帽结合蛋白(cap binding protein)调控乙烯信号转导的分子机制
- 批准号:
- 批准年份:2021
- 资助金额:58 万元
- 项目类别:
ID1 (Inhibitor of DNA binding 1) 在口蹄疫病毒感染中作用机制的研究
- 批准号:31672538
- 批准年份:2016
- 资助金额:62.0 万元
- 项目类别:面上项目
番茄EIN3-binding F-box蛋白2超表达诱导单性结实和果实成熟异常的机制研究
- 批准号:31372080
- 批准年份:2013
- 资助金额:80.0 万元
- 项目类别:面上项目
P53 binding protein 1 调控乳腺癌进展转移及化疗敏感性的机制研究
- 批准号:81172529
- 批准年份:2011
- 资助金额:58.0 万元
- 项目类别:面上项目
DBP(Vitamin D Binding Protein)在多发性硬化中的作用和相关机制的蛋白质组学研究
- 批准号:81070952
- 批准年份:2010
- 资助金额:35.0 万元
- 项目类别:面上项目
研究EB1(End-Binding protein 1)的癌基因特性及作用机制
- 批准号:30672361
- 批准年份:2006
- 资助金额:24.0 万元
- 项目类别:面上项目
相似海外基金
I-Corps: Translation Potential of Real-time, Ultrasensitive Electrical Transduction of Biological Binding Events for Pathogen and Disease Detection
I-Corps:生物结合事件的实时、超灵敏电转导在病原体和疾病检测中的转化潜力
- 批准号:
2419915 - 财政年份:2024
- 资助金额:
$ 6.69万 - 项目类别:
Standard Grant
Modelling drug binding to biological ion channels
模拟药物与生物离子通道的结合
- 批准号:
2747257 - 财政年份:2022
- 资助金额:
$ 6.69万 - 项目类别:
Studentship
Elucidation of biological functions of the NCBP3 RNA-binding protein using a novel mutant mouse strain
使用新型突变小鼠品系阐明 NCBP3 RNA 结合蛋白的生物学功能
- 批准号:
22K06065 - 财政年份:2022
- 资助金额:
$ 6.69万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Identifying binding partners, biological substrates and antisense oligonucleotides regulating expression of short and long ACE2.
识别调节短和长 ACE2 表达的结合伴侣、生物底物和反义寡核苷酸。
- 批准号:
BB/V019848/1 - 财政年份:2021
- 资助金额:
$ 6.69万 - 项目类别:
Research Grant
Structure and function of pufferfish toxin, tetrodotoxin, binding proteins as biological defense agent
河豚毒素、河豚毒素、结合蛋白作为生物防御剂的结构和功能
- 批准号:
19K06241 - 财政年份:2019
- 资助金额:
$ 6.69万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The molecular and biological roles of growth inhibiting chromatin binding proteins
生长抑制染色质结合蛋白的分子和生物学作用
- 批准号:
nhmrc : GNT1143612 - 财政年份:2018
- 资助金额:
$ 6.69万 - 项目类别:
Project Grants
Investigating a biological specificity conundrum: the role of dynamics in transcription factor binding
研究生物特异性难题:动力学在转录因子结合中的作用
- 批准号:
406750 - 财政年份:2018
- 资助金额:
$ 6.69万 - 项目类别:
Studentship Programs
Biological effect and preventive method for human serum albumin binding to transboundary air borne PM2.5.
人血清白蛋白与跨境空气PM2.5结合的生物学效应及预防方法。
- 批准号:
18H03039 - 财政年份:2018
- 资助金额:
$ 6.69万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The molecular and biological roles of growth inhibiting chromatin binding proteins
生长抑制染色质结合蛋白的分子和生物学作用
- 批准号:
nhmrc : 1143612 - 财政年份:2018
- 资助金额:
$ 6.69万 - 项目类别:
Project Grants
Electrical Detection of Small Molecule Binding to Biological Receptors using Organic Thin Film Transistors : A new approach for label free assays
使用有机薄膜晶体管对小分子与生物受体结合的电检测:一种无标记测定的新方法
- 批准号:
133593 - 财政年份:2018
- 资助金额:
$ 6.69万 - 项目类别:
Feasibility Studies