STUDYING NATURAL AND DESIGNED RED-LIGHT PHOTORECEPTORS BY STATIC AND DYNAMIC

通过静态和动态研究天然和设计的红光感光器

基本信息

  • 批准号:
    8363682
  • 负责人:
  • 金额:
    $ 6.69万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2011
  • 资助国家:
    美国
  • 起止时间:
    2011-08-01 至 2012-07-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Light is a fundamental signal that regulates important physiological processes such as development and circadian rhythm in living organisms. Phytochromes form a major family of photoreceptors responsible for red light perception in plants fungi and bacteria1. They undergo reversible photoconversion between red-absorbing (Pr) and far-red-absorbing (Pfr) states thereby ultimately converting a light signal into a distinct biological signal that mediates subsequent cellular responses. Our research aims to understand the molecular mechanisms of Pr/Pfr photoconversion and signal transduction in phytochromes using representative bacteriophytochromes (BphP) from P. aeruginosa and R. palustris. BphPs consist of three N-terminal photosensory domains (PAS GAF and PHY) and a C-terminal histidine kinase (HK) effector domain in which the HK activity is regulated by light signals detected by the photosensory core domains (PCD). During previous proposal periods we have determined several crystal structures of PCD in the Pr and Pfr states respectively. We have also captured three early reaction intermediates by initiating and following the Pfr-to-Pr photoreaction in photoactive PaBphP crystals using temperature-scanning cryo-crystallography. Taken together these crystal structures of reactant product and intermediate states provide structural insight into initial molecular events of BphPs upon sensing red-light and subsequent conformational changes in the chromophore and adjacent protein matrix. During the next period (1) we plan to extend our crystallographic studies to full-length (FL) BphPs which include a C-terminal hisitidine kinase (HK) effector domain. We aim to identify tertiary and quaternary structural elements that are responsible for transmitting light-induced local conformational changes in the chromophore-binding pocket to the remote HK to generate a biological signal. (2) We will further explore time-resolved experimental strategies based on both Laue and monochromatic diffraction to study reaction intermediates on various time-scales (from ps to ms) in collaboration with beamline scientists at BioCARS. (3) We will carry out crystallographic studies on artificial photoreceptors if suitable projects/crystals arise in couple with our ongoing protein design efforts to convey light-sensitivity to otherwise light-inert systems such as ion channels.
这个子项目是利用这些资源的众多研究子项目之一

项目成果

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JOHN Keith MOFFAT其他文献

JOHN Keith MOFFAT的其他文献

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{{ truncateString('JOHN Keith MOFFAT', 18)}}的其他基金

BioCARS: Structural Dynamics and Biological Mechanisms
BioCARS:结构动力学和生物机制
  • 批准号:
    10093063
  • 财政年份:
    2019
  • 资助金额:
    $ 6.69万
  • 项目类别:
Structures, Dynamics and Signaling Mechanisms of Bacteriophytochromes
细菌光敏色素的结构、动力学和信号机制
  • 批准号:
    8842642
  • 财政年份:
    2014
  • 资助金额:
    $ 6.69万
  • 项目类别:
Structures, Dynamics and Signaling Mechanisms of Bacteriophytochromes
细菌光敏色素的结构、动力学和信号机制
  • 批准号:
    8672967
  • 财政年份:
    2014
  • 资助金额:
    $ 6.69万
  • 项目类别:
BioCARS: A Synchrotron Structure Biology Resource
BioCARS:同步加速器结构生物学资源
  • 批准号:
    8735172
  • 财政年份:
    2013
  • 资助金额:
    $ 6.69万
  • 项目类别:
BioCARS: A Synchrotron Structure Biology Resource
BioCARS:同步加速器结构生物学资源
  • 批准号:
    8727171
  • 财政年份:
    2013
  • 资助金额:
    $ 6.69万
  • 项目类别:
High Speed Detector for Time-Resolved Research at BioCARS
BioCARS 用于时间分辨研究的高速探测器
  • 批准号:
    7836715
  • 财政年份:
    2011
  • 资助金额:
    $ 6.69万
  • 项目类别:
Time-Resolved Macromolecular Crystallography
时间分辨高分子晶体学
  • 批准号:
    8035651
  • 财政年份:
    2010
  • 资助金额:
    $ 6.69万
  • 项目类别:
EXPLORING LIGHT-SENSING AND SIGNALING MECHANISMS OF BACTERIOPHYTOCHROMES BY C
利用 C 探索细菌植物色素的光传感和信号传导机制
  • 批准号:
    8171982
  • 财政年份:
    2010
  • 资助金额:
    $ 6.69万
  • 项目类别:
TECH R&D CORE SUPPORT FOR AIDS RESEARCH
技术研发
  • 批准号:
    8172025
  • 财政年份:
    2010
  • 资助金额:
    $ 6.69万
  • 项目类别:
EXPLORING LIGHT-SENSING AND SIGNALING MECHANISMS OF BACTERIOPHYTOCHROMES BY C
利用 C 探索细菌植物色素的光传感和信号传导机制
  • 批准号:
    7956811
  • 财政年份:
    2009
  • 资助金额:
    $ 6.69万
  • 项目类别:

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