Intravital point-scanning confocal microscope

活体点扫描共焦显微镜

• 批准号: 7794379
• 负责人: Ashutosh Chilkoti
• 金额: $ 49.98万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794379
• 关键词: Arts; Commit; Core Facility; Fluorescence Microscopy; Funding; Image; Lasers; Life; Microscope; Microscopy; Mus; Optics; Photons; Resources; Scanning; System; Universities; imaging modality; in vivo; light microscopy

DESCRIPTION (provided by applicant): This is a request for a 2-photon intravital laser scanning confocal fluorescence microscopy system -"intravital confocal microscope"- for a variety of projects involving confocal imaging of living mice. The system will be installed in the Light Microscopy Core Facility (LMCF), a University core facility that currently serves the optical microscopy needs of several hundred users from across the entire Duke University campus. At present users are substantially limited by the current imaging resources for in vivo imaging. The only upright confocal microscope available for intravital imaging is no longer supported by Zeiss and will need to be decommissioned in the next year or so, and the only 2-Photon confocal microscope available on campus is inverted, obsolete, slow, and is consequently very poorly suited to in vivo imaging. As evidence of Duke University's commitment to the acquisition of state-of- the-art in vivo imaging modalities, the University has committed funds up to $300,000 that would supplement the funds requested herein to enable the integration of 2-photon capability to the system.

描述（由申请人提供）：这是一个双光子活体激光扫描共聚焦荧光显微镜系统-“活体共聚焦显微镜”-用于涉及活体小鼠共聚焦成像的各种项目的请求。该系统将被安装在光学显微镜核心设施（LMCF），大学的核心设施，目前服务于整个杜克大学校园数百名用户的光学显微镜的需求。目前，用户基本上受到当前用于体内成像的成像资源的限制。蔡司不再支持唯一可用于活体成像的直立式共聚焦显微镜，并且需要在未来一年左右退役，并且校园内唯一的双光子共聚焦显微镜是倒置的，过时的，缓慢的，因此非常不适合活体成像。作为杜克大学致力于采购最先进的体内成像模式的证据，该大学已承诺提供高达30万美元的资金，以补充本文所要求的资金，从而能够将双光子能力集成到系统中。