EFFECTS OF HORMONES ON MUCOSAL IMMUNE MEDIATORS ACROSS THE MENSTRUAL CYCLE

激素对整个月经周期粘膜免疫介质的影响

• 批准号: 7718170
• 负责人: MARLA J KELLER
• 金额: $ 17.93万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7718170
• 关键词: Address; Age-Years; Biological; Biological Assay; Biological Response Modifiers; Cells; Computer Retrieval of Information on Scientific Projects Database; Data; Defensins; Development; Environment; Female; Funding; Future; Genital system; Grant; HIV; Hormonal; Hormones; Immune; Immunoglobulins; Individual; Infection; Institution; Lactoferrin; Liquid substance; Measures; Menstrual cycle; Menstruation; Mucosal Immunity; Oral Contraceptives; Pharmaceutical Preparations; Phase; Population; Pregnancy; Proteins; Relative (related person); Research; Research Personnel; Resources; Sampling; Sexually Transmitted Diseases; Source; United States National Institutes of Health; Vagina; Woman; Women' s Group; antileukoprotease; antimicrobial; chemokine; cohort; cytokine; hormonal contraception; human SLPI protein; microbicide; pathogen; prevent; response; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The female genital tract is immunologically unique because it must be tolerant to spermatozoa, pregnancy and vaginal flora, but also protect the host from pathogen challenge. Moreover, hormonal fluctuations may modify the mucosal environment. Cytokines, chemokines, immunoglobulins, defensins and other proteins provide a defense against sexually transmitted infections (STI), but also may be modified in the setting of infection or in response to vaginal application of microbicides, drugs in development to prevent HIV and other STI. Little is known about the impact of endogenous and exogenous hormones on the relative concentration of individual factors in cervicovaginal secretions and the cumulative effects of any observed changes on mucosal immunity. The proposed study will address this gap and provide critical data for ongoing and future studies that address the impact of microbicides on levels of cytokines, chemokines or other proteins and cell populations in cervicovaginal secretions. We propose to measure the changes in immune mediators in two groups of women, 18-35 years of age. One group will be using hormonal contraception and the other group will have regular menstrual periods. The biological significance of any observed changes will be assessed by examining the innate antimicrobial activity of the fluid using well-established assays. Hypothesis: To establish the variability in cytokines, chemokines, defensins, secretory leukocyte protease inhibitor (SLPI), immunoglobulins and lactoferrin in cervicovaginal fluids in a cohort of women 18-35 years of age at each phase of the menstrual cycle compared with women on oral contraceptives. To establish variability in immune cell population in cytobrush samples obtained from a cohort of women 18-35 years of age at each phase of the menstrual cycle compared with women on oral contraceptives . To determine if changes in mucosal immune mediators across the menstrual cycle or in women using oral contraceptives are associated with alterations in intrinsic antimicrobial activity

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 女性生殖道在免疫学上是独一无二的，因为它必须对精子、妊娠和阴道菌群具有耐受性，同时也要保护宿主免受病原体的攻击。此外，荷尔蒙波动可能会改变粘膜环境。细胞因子、趋化因子、免疫球蛋白、防御素和其他蛋白质提供对性传播感染(STI)的防御，但也可以在感染环境中或在阴道应用杀菌剂时进行修改，开发中的药物可以预防艾滋病毒和其他STI。关于内源性和外源性激素对宫颈阴道分泌物中单个因子的相对浓度的影响，以及任何观察到的变化对粘膜免疫的累积影响，人们知之甚少。这项拟议的研究将解决这一差距，并为正在进行的和未来的研究提供关键数据，这些研究旨在解决杀菌剂对宫颈阴道分泌物中细胞因子、趋化因子或其他蛋白质和细胞群水平的影响。我们建议在18-35岁的两组女性中测量免疫介质的变化。一组将使用荷尔蒙避孕，另一组将有规律的月经。任何观察到的变化的生物学意义将通过使用成熟的分析方法检查液体的固有抗微生物活性来评估。 假设： 目的：探讨18~35岁女性月经周期各期宫颈阴道液中细胞因子、趋化因子、防御素、分泌性白细胞蛋白酶抑制物(SLPI)、免疫球蛋白和乳铁蛋白与口服避孕药的差异。 目的：与口服避孕药的女性相比，在月经周期的每个阶段从18-35岁的女性队列中获取细胞刷样本，以建立免疫细胞群体的变异性。 确定在整个月经周期或使用口服避孕药的妇女中，粘膜免疫介质的变化是否与内在抗菌活性的变化有关