Advanced Lab Technologies Core

项目总结/摘要 先进实验室技术核心（核心I） 哈佛大学（HU）CFAR的最大附加值之一是加速了会员访问 新的和新兴的技术。科技的变化和心流的进化加速了科学的发展 细胞计数、成像和基因组技术继续快速发展。然而，获得这些技术 和相关的计算方法是一个不断增加的和具有挑战性的障碍，个人 investigators.购置和优化仪器和软件，并使其易于使用 HUCFAR先进实验室技术核心（ALTC）的主要重点。把这些切割- HU CFAR用户可用的边缘技术为他们的HIV储库研究增加了急需的价值 和治愈，病毒-宿主相互作用，治疗干预和疫苗开发。合并的ALTC 提供免疫学服务、分子生物学服务和培训机会的统一管道 具有战略地位，以最有效的财政价值为更大的哈佛社区服务。持续增 ALTC为数百名HU CFAR用户及其全球合作者提供服务， 在10个不同的NIH研究所和中心资助的赠款/合同。ALTC还继续关注 为早期职业调查员（ECI）提供关键的个性化技术支持，包括数十名HU CFAR发展和指导核心获奖者。目前，已确定的大部分需要 成员是捕获关于单个细胞的表型和功能状态的信息， 对这些样本进行索引，然后利用这种珍贵的生物材料来绘制全面的图像 存在于特定疾病状态中的基因组景观。为了确保所有哈佛大学的CFAR 实验室可以获得这种非凡的分析能力，这个核心将有助于获得这种 通过以下目标实现技术： 1)为HU CFAR成员提供最先进的仪器和技术。 2)提供所有ALTC技术的用户培训以及计算协助。 3)使用最先进的共享和分发工具建立一个虚拟社区。

PROJECT SUMMARY/ABSTRACT Advanced Lab Technologies Core (Core I) One of the greatest added values of the Harvard University (HU) CFAR has been accelerated member access to new and emerging technologies. Science is accelerated by changes in technology, and the evolution of flow cytometric, imaging and genomic techniques continues at a rapid pace. However, access to such technologies and associated computational methodologies is an ever increasing and challenging barrier to individual investigators. Acquisition and optimization of instrumentation and softwares and making them accessible remains the primary focus of the HU CFAR Advanced Lab Technologies Core (ALTC). Making these cutting- edge technologies available to HU CFAR users adds critically needed value to their studies of HIV reservoir and cure, virus-host interactions, therapeutic interventions, and vaccine development. The consolidated ALTC offers a unified pipeline of immunology services, molecular biology services, and training opportunities strategically placed to serve the greater Harvard community at the most effective fiscal value. Indeed, the ALTC serves hundreds of HU CFAR users and their global collaborators supported by more than 100 NIH- funded grants/contracts across ten different NIH Institutes and Centers. The ALTC also continues to focus on providing critical individualized technical support to Early Career Investigators (ECI), including dozens of HU CFAR Developmental and Mentoring Core awardees. At the current time, much of the identified need for members is in the capture of information regarding the phenotypic and functional state of individual cells, indexing these samples, and then capitalizing on this precious biomaterial to develop a comprehensive picture of the genomic landscape that exists in particular disease states. To ensure that all Harvard University CFAR laboratories have access to this remarkable analytical power, this core will facilitate access to such technologies through the following aims: 1) Provide HU CFAR member access to state-of-the art instrumentation and techniques. 2) Provide user training in all ALTC technologies, as well as computational assistance. 3) Build a virtual community using state of the art sharing and distribution tools.