NCCAT: National Center for CryoEM Access and Training

NCCAT：国家冷冻电镜访问和培训中心

• 批准号: 10244409
• 负责人: BRIDGET Olivia CARRAGHER
• 金额: $ 200万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10244409
• 关键词: Cryoelectron Microscopy; Electrons; Ensure; Equipment; Funding; Goals; Macromolecular Complexes; Methods; Microscope; Modeling; New York; Preparation; Production; Resolution; Robot; Source; Specimen; Structure; Time; Titan; Training; Training Activity; Training Programs; base; biological systems; career; detector; next generation; particle; screening; skills; structural biology

Summary The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team provides direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. A cross-training program provides training to users across a wide variety of skill levels and career goals. This is a request for supplemental funding for the National Center for CryoEM Access and Training (NCCAT) to upgrade a Titan Krios microscope with a next generation electron source and energy filter. Additional supplemental funding is requested for a 120 KeV TEM that will be used for training activities at NCCAT.

总结 位于纽约结构生物学中心的国家冷冻EM获取和培训中心（NCCAT）， 提供最先进的设备，包括标本制备机器人、高端显微镜 以及直接检测器、筛选显微镜以及解析结构所需的所有其他辅助设备 使用冷冻电子显微镜（cryoEM）方法获得最高分辨率。一个高素质的技术 团队提供直接的支持、指导和帮助，以确保最高质量的结构 在最短的时间内生产。交叉培训计划为各种用户提供培训 技能水平和职业目标。 这是对国家冷冻EM获取和培训中心（NCCAT）的补充资金申请， 使用下一代电子源和能量过滤器升级Titan Krios显微镜。额外 要求为将用于NCCAT培训活动的120 KeV TEM提供补充资金。