NCCAT: National Center for CryoEM Access and Training

总结 位于纽约结构生物学中心的国家冷冻EM获取和培训中心（NCCAT）， 提供最先进的设备，包括标本制备机器人、高端显微镜 直接探测器，筛选显微镜，以及所有其他辅助设备， 使用冷冻电子显微镜（cryoEM）方法获得最高分辨率。一个高素质的技术 团队提供直接的支持、指导和帮助，以确保最高质量的结构 在最短的时间内生产。交叉培训计划为各种用户提供培训 技能水平和职业目标。 这是对国家冷冻EM获取和培训中心（NCCAT）的补充资金申请， 升级Titan Krios显微镜，配备下一代电子源和能量过滤器。额外 要求为将用于NCCAT培训活动的120 KeV TEM提供补充资金。

Summary The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team provides direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. A cross-training program provides training to users across a wide variety of skill levels and career goals. This is a request for supplemental funding for the National Center for CryoEM Access and Training (NCCAT) to upgrade a Titan Krios microscope with a next generation electron source and energy filter. Additional supplemental funding is requested for a 120 KeV TEM that will be used for training activities at NCCAT.