NCCAT: National Center for CryoEM Access and Training- Supplement for Windows 10 and FFI

NCCAT：国家 CryoEM 访问和培训中心 - Windows 10 和 FFI 的补充

• 批准号: 10579565
• 负责人: BRIDGET Olivia CARRAGHER
• 金额: $ 31.83万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10579565
• 关键词: Computer software; Computers; Cryoelectron Microscopy; Data; Ensure; Equipment; Funding; Goals; Ions; Lighting; Macromolecular Complexes; Methods; Microscope; Modeling; New York; Preparation; Production; Resolution; Robot; Running; Security; Specimen; Structure; System; Time; Titan; Training; Training Programs; Update; base; biological systems; career; detector; improved; instrument; particle; screening; skills; structural biology

Project Summary/Abstract The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team provides direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. A cross-training program provides training to users across a wide variety of skill levels and career goals. We are requesting funds to purchase fringe free illumination (FFI) and Windows10 computer upgrades for three Thermo Fisher Scientific (TFS) Titan Krios G3i instruments (Krios4, Krios5, Krios6) at the NCCAT. FFI considerably improves the throughput of data collect ion, and these upgrades will benefit all users of NCCAT. The Windows 10 upgrade increase the security of all of our systems which currently run Windows 7, which has many known security issues and is no longer officially supported as of January 2020. This means that updates to software, security fixes and technical support are no longer provided.

项目总结/摘要 位于纽约结构生物学中心的国家冷冻EM获取和培训中心（NCCAT）， 提供最先进的设备，包括标本制备机器人、高端显微镜 直接探测器，筛选显微镜，以及所有其他辅助设备， 使用冷冻电子显微镜（cryoEM）方法获得最高分辨率。一个高素质的技术 团队提供直接的支持、指导和帮助，以确保最高质量的结构 在最短的时间内生产。交叉培训计划为各种用户提供培训 技能水平和职业目标。 我们正在申请资金购买无条纹照明（FFI）和Windows10计算机升级， NCCAT的Thermo Fisher Scientific（TFS）Titan Krios G3 i仪器（Krios 4、Krios 5、Krios 6）。FFI 大大提高了数据收集的吞吐量，这些升级将使NCCAT的所有用户受益。 Windows 10升级提高了我们目前运行Windows 7的所有系统的安全性， 许多已知的安全问题，自2020年1月起不再受到官方支持。这意味着更新 对于软件，不再提供安全补丁和技术支持。