NCITU: National Center for In-situ Tomographic Ultramicroscopy. Supplement

摘要 国家原位层析超微中心(NCITU)设在西蒙斯电子公司 显微镜中心(SEMC)，这是纽约结构生物学中心(NYSBC)的一部分，总部设在纽约 纽约州约克市NCITU致力于开发和提供访问标准和高级方法的途径 冷冻ET标本的原位制备，并通过冷冻ET交叉培训传播这一专门知识 该计划旨在服务于各种技能水平和职业目标。 国家原位层析超微中心(NCITU)的主要目标是为用户提供 获取和服务于制备用于直接观察大分子缔合和 组织，特别是在原生细胞中。一支高素质的技术团队提供直接的实践服务， 指导和协助，以确保样品和格栅以最高质量制备，并在 尽可能短的时间。一个已经建立良好的交叉培训计划正在扩大和延伸到 提供有关低温样品制备管道各个方面的培训。 这是为国家原位断层扫描超微中心提供补充资金的请求 (NCITU)，以增强中心的数据分析和Clem能力。这是一项具体请求，要求(I) (2)Zeiss LSM 900软件升级；(3)第二个Linkam CMS196低温克莱姆舞台。

Summary The National Center for In-situ Tomographic Ultramicroscopy (NCITU) is based at the Simons Electron Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New York, NY. NCITU is dedicated to developing and providing access to both standard and advanced methods for in-situ cryoET specimen preparation, and to disseminating this expertise through a cryoET cross-training program designed to serve a wide variety of skill levels and career goals. The primary goal of the National Center for In-situ Tomographic Ultramicroscopy (NCITU) is to provide user access and service towards preparing specimen for direct visualization of macromolecular associations and organization, particularly in native cells. A highly qualified technical team provides direct hands-on service, guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the shortest time possible. An already well established cross-training program is being expanded and extended to provide training in every aspect of the cryoET specimen preparation pipeline. This is a request for supplemental funding for the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to enhance the Center's data analysis and CLEM capabilities. This is a specific request for (i) a dedicated cryoET processing workstation, (ii) a Zeiss LSM 900 software upgrade, and (iii) a second Linkam CMS196 cryo CLEM stage.