The National Center for In-situ Tomographic Ultramicroscopy (NCITU)

国家原位断层超显微术中心 (NCITU)

• 批准号: 10397367
• 负责人: BRIDGET Olivia CARRAGHER
• 金额: $ 24.66万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10397367
• 关键词: Cells; Electron Microscopy; Ensure; Equipment; Funding; Goals; In Situ; Liquid substance; Longevity; Methods; New York; Nitrogen; Preparation; Rod; Sampling; Services; Specimen; Time; Training; Training Programs; Vacuum; Visualization; base; career; design; method development; skills; structural biology; tomography; water vapor

Summary We propose to establish the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to be based at the Simons Electron Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New York, NY. NCITU is dedicated to developing and providing access to both standard and advanced methods for in-situ cryoET specimen preparation, and to disseminating this expertise through a cryoET cross-training program designed to serve a wide variety of skill levels and career goals. The primary goal of the National Center for In-situ Tomographic Ultramicroscopy (NCITU) is to provide user access and service towards preparing specimen for direct visualization of macromolecular associations and organization, particularly in native cells. A highly qualified technical team provides direct hands-on service, guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the shortest time possible. An already well established cross-training program is being expanded and extended to provide training in every aspect of the cryoET specimen preparation pipeline. This is a request for supplemental funding for the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to enhance the quality and throughput of the TFS Aquilos 2 cryo-FIB-SEM. This is a specific request for (i) a high-vacuum sample transfer rod, (ii) an in-chamber water vapor reduction upgrade, and (iii) a liquid nitrogen cooling longevity upgrade.

摘要 我们建议建立国家原位断层扫描超微中心(NCITU)，总部设在 西蒙斯电子显微镜中心(SEMC)，这是纽约结构生物学中心的一部分 (NYSBC)，总部设在纽约。NCITU致力于开发和提供访问标准和 原位低温样品制备的先进方法，并通过低温ET传播这一专门知识 旨在为各种技能水平和职业目标服务的交叉培训计划。 国家原位层析超微中心(NCITU)的主要目标是为用户提供 获取和服务于制备用于直接观察大分子缔合和 组织，特别是在原生细胞中。一支高素质的技术团队提供直接的实践服务， 指导和协助，以确保样品和格栅以最高质量制备，并在 尽可能短的时间。一个已经建立良好的交叉培训计划正在扩大和延伸到 提供有关低温样品制备管道各个方面的培训。 这是为国家原位断层扫描超微中心提供补充资金的请求 (NCITU)，以提高TFS Aquilos 2 Cryo-FIB-SEM的质量和产量。这是一个特定的请求 用于(I)高真空样品转移棒，(Ii)室内水蒸气减量升级，和(Iii)液体 氮气冷却寿命升级。