The National Center for In-situ Tomographic Ultramicroscopy (NCITU)

总结 我们建议建立国家原位层析超显微镜中心（NCITU）， 西蒙斯电子显微镜中心（SEMC）是纽约结构生物学中心的一部分 （NYSBC），总部位于纽约州纽约。NCITU致力于开发和提供标准和 原位冷冻ET标本制备的先进方法，并通过冷冻ET传播这种专业知识， 交叉培训计划，旨在服务于各种技能水平和职业目标。 国家原位层析超显微镜中心（NCITU）的主要目标是为用户提供 为直接观察大分子缔合物制备样品提供访问和服务， 组织，特别是在原生细胞中。高素质的技术团队提供直接的实践服务， 指导和协助，以确保样品和网格以最高质量制备， 尽可能短的时间。一个已经很好建立的交叉培训计划正在扩大和延伸， 提供cryoET标本制备管道各个方面的培训。 这是一份为国家原位断层扫描超显微镜中心提供补充资金的申请 （NCITU），以提高TFS Aquilos 2 cryo-FIB-SEM的质量和通量。这是一个特别的要求 用于（i）高真空样品转移棒，（ii）室内水蒸气减少升级，和（iii）液体 氮气冷却寿命升级。

Summary We propose to establish the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to be based at the Simons Electron Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New York, NY. NCITU is dedicated to developing and providing access to both standard and advanced methods for in-situ cryoET specimen preparation, and to disseminating this expertise through a cryoET cross-training program designed to serve a wide variety of skill levels and career goals. The primary goal of the National Center for In-situ Tomographic Ultramicroscopy (NCITU) is to provide user access and service towards preparing specimen for direct visualization of macromolecular associations and organization, particularly in native cells. A highly qualified technical team provides direct hands-on service, guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the shortest time possible. An already well established cross-training program is being expanded and extended to provide training in every aspect of the cryoET specimen preparation pipeline. This is a request for supplemental funding for the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to enhance the quality and throughput of the TFS Aquilos 2 cryo-FIB-SEM. This is a specific request for (i) a high-vacuum sample transfer rod, (ii) an in-chamber water vapor reduction upgrade, and (iii) a liquid nitrogen cooling longevity upgrade.