NCCAT: National Center for CryoEM Access and Training- Supplement for Screening

项目摘要/摘要 总部设在纽约结构生物学中心的国家低温电子显微镜获取和培训中心(NCCAT)， 提供最先进的设备，包括标本准备机器人、高端显微镜 以及直接探测器、筛选显微镜以及解决结构问题所需的所有其他辅助设备 使用低温电子显微镜(CryoEM)方法获得尽可能高的分辨率。一位高素质的技术人员 团队提供直接支持、指导和协助，以确保最高质量的结构 在尽可能短的时间内完成。交叉培训计划为各种不同的用户提供培训 技能水平和职业目标。 这是一项补充资金申请，以支持低温网格准备和筛选(GPS)服务。 NCCAT的TEM。其目标是为经验有限的群体提供急需的支持 使用冷冻EM的方法，并限制进入当地设施。我们的目标将是支持这些团体 让他们开始，同时在所有可能的方面对他们进行培训，以便他们能够最大限度地利用 尽快为他们提供所有可用的资源。

Project Summary/Abstract The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team provides direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. A cross-training program provides training to users across a wide variety of skill levels and career goals. This is a request for supplemental funding to support a grid preparation and screening (GPS) service for cryo- TEM at NCCAT. The objective is to provide urgently needed support to groups who have limited experience with the methods of cryoEM and limited access to local facilities. The goal will be to support these groups to get them started while also training them in all possible aspects so that they can make best possible use of every resource available to them as soon as possible.