NCCAT: National Center for CryoEM Access and Training- Supplement for Screening

项目总结/摘要 位于纽约结构生物学中心的国家冷冻EM获取和培训中心（NCCAT）， 提供最先进的设备，包括标本制备机器人、高端显微镜 直接探测器，筛选显微镜，以及所有其他辅助设备， 使用冷冻电子显微镜（cryoEM）方法获得最高分辨率。一个高素质的技术 团队提供直接的支持、指导和帮助，以确保最高质量的结构 在最短的时间内生产。交叉培训计划为各种用户提供培训 技能水平和职业目标。 这是一项补充资金的请求，以支持一个网格准备和筛选（GPS）服务的低温- 在NCCAT。目标是向经验有限的团体提供迫切需要的支助 冷冻电镜技术和有限的当地设施。目标是支持这些团体， 让他们开始，同时也在所有可能的方面培训他们，使他们能够最好地利用 尽可能快地提供所有可用的资源。

Project Summary/Abstract The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team provides direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. A cross-training program provides training to users across a wide variety of skill levels and career goals. This is a request for supplemental funding to support a grid preparation and screening (GPS) service for cryo- TEM at NCCAT. The objective is to provide urgently needed support to groups who have limited experience with the methods of cryoEM and limited access to local facilities. The goal will be to support these groups to get them started while also training them in all possible aspects so that they can make best possible use of every resource available to them as soon as possible.