Center for Solutions for ME/CFS

ME/CFS 解决方案中心

• 批准号: 10246404
• 负责人: W. Ian Lipkin
• 金额: $ 184.33万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-22 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10246404
• 关键词: Abbreviations; Advisory Committees; Algorithms; Analytical Chemistry; Autoantibodies; Basic Science; Biochemistry; Biological; Biological Assay; Biological Markers; Biology; Blood specimen; Caregivers; Cells; Centers for Disease Control and Prevention (U.S.); Chemical Structure; Chronic; Chronic Fatigue Syndrome; Clinical; Clinical Investigator; Collection; Communicable Diseases; Communities; Complex; Consultations; Crowding; Data; Data Scientist; Databases; Diagnosis; Diagnostic; Diagnostic Procedure; Diagnostic tests; Disease; Edible Plants; Emerging Communicable Diseases; Ensure; Epidemiologist; Epidemiology; Epigenetic Process; Etiology; Exercise; Exercise Test; Exercise Tolerance; Family; Fatigue; Fibromyalgia; Flavonoids; Foundations; Frequencies; Funding; Gene Expression; Genetic; Genomics; High-Throughput Nucleotide Sequencing; Immune; Immune response; Immunity; Immunology; Infection; Interdisciplinary Study; International; Intervention; Intramural Research Program; Investigation; Investments; Laboratories; Laboratory Scientists; Lead; Lesion; Libraries; Link; Lipids; Literature; Metabolic Pathway; Methods; Microbe; Microbiology; Mining; Molecular; Murine leukemia virus; National Institute of Neurological Disorders and Stroke; North America; Oral; Outpatients; Pathogenesis; Patient Care; Patients; Peptides; Peripheral Blood Mononuclear Cell; Phenotype; Physicians; Plasma; Population; Positioning Attribute; Prevalence; Procedures; Proteomics; RNA; Reporting; Research; Research Personnel; Research Project Grants; Resources; Risk; Role; Sampling; Scientist; Security; Serology; Source; Standardization; Study Subject; Surveys; Systems Biology; Testing; Thinness; Translational Research; United States National Institutes of Health; Universities; Viral; Vision; Work; base; biodefense; biomarker discovery; cheminformatics; clinical database; cohort; comorbidity; computerized data processing; crowdsourcing; data management; density; design; education research; evidence base; flexibility; functional genomics; holistic approach; insight; instrument; inter-institutional; member; metabolome; metabolomics; microbial; microbiome; mobile application; polyphenol; pressure; prevent; programs; prospective; public repository; repository; response; stressor; tool; transcriptome; transcriptome sequencing; transcriptomics

Overall Abstract The Center for Solutions for ME/CFS (CfS for ME/CFS) is an inter-disciplinary, inter-institutional center comprised of clinicians, clinical investigators, basic scientists who are committing to working together to understand the pathogenesis of ME/CFS and develop evidence-based strategies for interventions that prevent and mitigate disease. The team initially coalesced with an NIH call to respond to spurious reports linking xenotropic murine leukemia virus-related virus (XMRV) to ME/CFS. It consolidated its vision with support from the Hutchins Family Foundation Chronic Fatigue Initiative (CFI) and a crowd-funding organization, The Microbe Discovery Project, to explore the role of infection and immunity in disease and identify biomarkers for diagnosis through functional genomic, proteomic, and metabolomic discovery. The foundation for this project is a large clinical database and sample repository representing oral, fecal, and blood samples from well-characterized ME/CFS subjects and frequency-matched controls collected nationwide over a period of several years. In Project 1, we survey for the presence of molecular footprints of bacterial, fungal, and viral agents and corresponding immune responses in a 100 case/100 control subset of repository samples using high throughput sequencing, high density peptide arrays, and immune signature assays; this Project also examines the prevalence of autoantibodies selected based on clinical and literature reports. In Project 2, we profile the plasma metabolome and PBMC transcriptome in the same subjects studied in Project 1 using state-of-the-art mass spectrometric and RNA-seq methods, comprehensive mass spectral libraries, and tools for RNA profiling in bulk cell populations using cell sub-type specific markers. We also pursue metabolomic and transcriptomic analyses after orthostatic and exercise challenges using samples collected in Project 3. In Project 3, we mine existing databases at Columbia and the CFI for insights into clinical features, comorbidities, and sub-types that can be used to refine laboratory analyses, as well as enhance patient care. We will work with clinicians and the ME/CFS community to design a mobile app that will allow patients and caregivers to track clinical status in response to stressors and interventions, and will provide prospective research data. We will also investigate the utility of the Lean Test as a simple outpatient test for autonomic function.

整体 摘要 ME/CFS解决方案中心（CfS for ME/CFS）是一个跨学科、跨机构的中心。 由临床医生、临床研究者、基础科学家组成，他们致力于共同努力， 了解ME/CFS的发病机制，并制定以证据为基础的干预策略， 减轻疾病。该团队最初与NIH呼吁联合起来，以回应虚假的报告， 异嗜性小鼠白血病病毒相关病毒（XMRV）至ME/CFS。它在以下方面的支持下巩固了自己的愿景： 哈钦斯家庭基金会慢性疲劳倡议（CFI）和一个众筹组织，微生物 探索项目，探索感染和免疫在疾病中的作用，并确定用于诊断的生物标志物 通过功能基因组学、蛋白质组学和代谢组学的发现。这个项目的基础是一个大型的 临床数据库和样本库，代表来自良好表征的口腔、粪便和血液样本 ME/CFS受试者和频率匹配的对照在全国范围内收集了几年的时间。在 项目1，我们调查细菌、真菌和病毒因子的分子足迹的存在， 在100个病例/100个对照子组的储存库样品中，使用高 通量测序，高密度肽阵列和免疫特征分析;该项目还检查 根据临床和文献报告选择自身抗体的患病率。在项目2中，我们分析了 在项目1中研究的相同受试者中使用最先进的血浆代谢组和PBMC转录组 质谱和RNA-seq方法、全面的质谱库和RNA分析工具 使用细胞亚型特异性标记在大量细胞群体中进行。我们还研究代谢组学和转录组学， 使用项目3中收集的样本进行立位和运动挑战后的分析。在项目3中， 哥伦比亚和CFI的现有数据库，以深入了解临床特征、合并症和亚型， 可用于完善实验室分析，以及加强病人护理。我们将与临床医生和 ME/CFS社区设计一个移动的应用程序，允许患者和护理人员跟踪临床状态， 对压力源和干预措施的反应，并将提供前瞻性的研究数据。我们亦会研究 精益测试作为一种简单的自主功能门诊测试的效用。