Proteomics and Mass Spectrometry

蛋白质组学核心摘要 这个由国家眼科研究所资助的蛋白质组学核心旨在利用蛋白质组学领域的现代技术 发现眼病的原因并改进治疗方法，包括白内障，青光眼，视网膜疾病， 干眼症和甲状腺相关眼病。蛋白质组学分析的具体目的是 测量蛋白质丰度和疾病修饰的变化，确定蛋白质如何折叠， 相互作用，以及它们如何调节发育。使用的方法将包括串联质量 标记以检测经历丰度变化的蛋白质，磷酸肽分析以发现 控制细胞过程的机制，氢-氘交换和化学交联， 确定结构。这些结果将补充该P30支持的其他内核生成的数据 拨款提高OHSU视觉研究人员的生产力，并设计更好的眼病治疗方法。 Kirsten Lampi博士作为共同主要研究者的加入将通过增加对以下方面的支持来扩大该核心的覆盖范围 由于她在氢/氘交换和蛋白质交联方面的专业知识，她进行了结构研究。

Proteomics Core Summary This National Eye Institute-funded Proteomics Core aims to use modern technologies in the field of proteomics to discover the causes and improve treatments for eye diseases, including cataract, glaucoma, retinal disease, dry eye, and thyroid-associated eye disease. Proteomic analyses will be performed with the specific aims of measuring changes in protein abundance and modification with disease, determining how proteins fold and interact with one another, and how they regulate development. The methods used will include tandem mass tagging to detect proteins undergoing changes in abundance, phosphopeptide analysis to discover mechanisms controlling cellular processes, hydrogen-deuterium exchange and chemical cross-linking to determine structure. The results will complement data generated by the other cores supported by this P30 grant to increase the productivity of vision researchers at OHSU and devise better treatments for eye disease. Addition of Dr. Kirsten Lampi as co-PI will broaden the reach of this core by providing increased support for structural studies due to her expertise in hydrogen/deuterium exchange and protein cross -linking.