LTQ XL Linear Ion Trap Mass Spectrometer

描述（由申请人提供）：我们要求将一个带有电子转移解离源的ThermoFisher LTQ XL线性离子阱放置在俄勒冈健康与科学大学的蛋白质组学共享资源（PSR）中。由于高灵敏度和快速扫描速率，该仪器将用于鉴定蛋白质/蛋白质或蛋白质/药物相互作用研究中沉淀蛋白质的复杂混合物中的蛋白质，亚细胞组分的制备，微解剖组织的提取物和免疫耗尽的血清样品。电子转移解离源将用于改进翻译后修饰的检测和定位，包括天冬氨酸的磷酸化、糖基化和异构化。该仪器将在12个研究人类疾病病因的不同项目中发挥关键作用，这些疾病包括白内障、听力丧失、年轻人和老年人的神经退行性疾病、癌症、甲基苯丙胺成瘾、血色素沉着症和糖尿病。该仪器的引入将满足俄勒冈健康与科学大学研究人员将最先进的蛋白质组学工具纳入其研究的日益增长的需求。这将加快他们的发现进程，以改进人类疾病的检测和治疗。公共卫生相关性：我们申请资助LTQ XL离子阱质谱仪来鉴定蛋白质并检测它们的修饰。该仪器将被放置在俄勒冈健康与科学大学的共享核心设施中，并将帮助调查从癌症到听力损失等疾病的病因和治疗方法。

DESCRIPTION (provided by applicant): We request a ThermoFisher LTQ XL linear ion trap with an electron transfer dissociation source to be placed in the Oregon Health & Science University's Proteomics Shared Resource (PSR). Due to the high sensitivity and fast scan rates, this instrument will be used to identify proteins in complex mixtures of precipitated proteins in Protein/Protein or Protein/Drug interaction studies, preparations of subcellular fractions, extracts of microdissected tissues, and immuno-depleted serum samples. The electron transfer dissociation source will be used to improve the detection and localization of post-translational modifications, including phosphorylation, glycosylation and isomerization of aspartate. The instrument will play a crucial role in 12 diverse projects studying the causes of human disease including cataracts, hearing loss, neurodegenerative disease in the young and old, cancer, methamphetamine addiction, hemochromatosis, and diabetes. The introduction of this instrument will fulfill the growing needs of the researchers at the Oregon Health & Sciences University to incorporate state-of-the-art tools in proteomics into their research. This will speed their progress of discovery to improve the detection and treatment of human disease. PUBLIC HEALTH RELEVANCE: We request funding for an LTQ XL ion trap mass spectrometer to identify proteins and detect their modifications. This instrument will be placed in a shared core facility and will help investigate the cause and treatment of diseases ranging from cancer to hearing loss at the Oregon Health & Science University.