Orbitrap Tribrid Mass Spectrometer

项目摘要 我们请求下一代Orbitrap Tribrid Orbitrap月食仪器放置在俄勒冈州健康和 理科大学蛋白质组学共享资源(PSR)。这一新工具将更好地满足 该大学的研究人员由于其更高的灵敏度，使用串联质量进行更准确的定量 标记技术，改进的电子离解源，以及FAIMS设计，以增加 复杂混合物中的蛋白质组学分析。该仪器将支持OHSU的新实验，推动 蛋白质组学的边界：允许分析单细胞蛋白质组，定位翻译后 膜蛋白受体中的修饰和药物结合部位，更准确和灵敏 测量复杂组织和细胞裂解物中的差异蛋白质丰度，测量新合成的 激光捕获显微切割分离组织中的蛋白质，进行氢/氢交换 测量，并通过化学交联实验提炼低温EM衍生的蛋白质结构。这个 仪器将支持对多种疾病的研究，包括癌症、白内障、青光眼、药物 上瘾，还有听力损失。该工具将允许PSR将其服务扩展到越来越多的 该大学的蛋白质组学研究人员需要获得最先进的质谱学 支持他们的研究需要的仪器。

Project Summary We request the next generation Orbitrap Tribrid Orbitrap Eclipse instrument to place in the Oregon Health & Science University Proteomics Shared Resource (PSR). The new instrument will better serve the needs of researchers at the university due to its greater sensitivity, more accurate quantitation using tandem mass tagging technology, improved electron dissociation source, and FAIMS devise to increase the depth of proteomic analysis in complex mixtures. The instrument will support new experiments at OHSU pushing the boundaries of proteomics by: allowing the analysis of single cell proteomes, localizing post-translational modifications and drug binding sites in membrane protein receptors, more accurately and sensitively measuring differential protein abundance in complex tissue and cellular lysates, measuring newly synthesized proteins in tissues isolated by laser-capture micro-dissection, performing hydrogen/deuterium exchange measurements, and refining cryo-EM derived protein structures using chemical cross-linking experiments. The instrument will support the study of a wide range of diseases, including cancer, cataracts, glaucoma, drug addiction, and hearing loss. The instrument will allow the PSR to expand its services to a growing group of proteomics researchers at the university who need access to the most advanced mass spectrometry instrumentation to support their research needs.