Orbitrap Tribrid Mass Spectrometer

Orbitrap 三合一质谱仪

• 批准号: 10175686
• 负责人: LARRY L DAVID
• 金额: $ 110.14万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-10 至 2022-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175686
• 关键词: Cataract; Chemicals; Complex; Complex Mixtures; Cryoelectron Microscopy; Deuterium Exchange Measurement; Disease; Dissociation; Drug Addiction; Drug Binding Site; Electrons; Glaucoma; Health Sciences; Malignant Neoplasms; Mass Spectrum Analysis; Measures; Membrane Proteins; Oregon; Post-Translational Protein Processing; Proteins; Proteome; Proteomics; Proteomics Shared Resource; Research; Research Personnel; Services; Source; Technology; Tissues; Universities; crosslink; experimental study; hearing impairment; improved; instrument; instrumentation; laser capture microdissection; mass spectrometer; next generation; protein structure; receptor; single cell analysis

Project Summary We request the next generation Orbitrap Tribrid Orbitrap Eclipse instrument to place in the Oregon Health & Science University Proteomics Shared Resource (PSR). The new instrument will better serve the needs of researchers at the university due to its greater sensitivity, more accurate quantitation using tandem mass tagging technology, improved electron dissociation source, and FAIMS devise to increase the depth of proteomic analysis in complex mixtures. The instrument will support new experiments at OHSU pushing the boundaries of proteomics by: allowing the analysis of single cell proteomes, localizing post-translational modifications and drug binding sites in membrane protein receptors, more accurately and sensitively measuring differential protein abundance in complex tissue and cellular lysates, measuring newly synthesized proteins in tissues isolated by laser-capture micro-dissection, performing hydrogen/deuterium exchange measurements, and refining cryo-EM derived protein structures using chemical cross-linking experiments. The instrument will support the study of a wide range of diseases, including cancer, cataracts, glaucoma, drug addiction, and hearing loss. The instrument will allow the PSR to expand its services to a growing group of proteomics researchers at the university who need access to the most advanced mass spectrometry instrumentation to support their research needs.

项目摘要 我们要求将下一代Orbitrap Tribrid Orbitrap Eclipse仪器放置在俄勒冈州健康与安全中心。 蛋白质组学共享资源（PSR）。新文书将更好地满足 该大学的研究人员由于其更高的灵敏度，更准确的定量使用串联质量 标签技术，改进的电子解离源，和FAIMS设计，以增加深度 蛋白质组分析在复杂的混合物。该仪器将支持OHSU的新实验， 蛋白质组学的边界：允许分析单细胞蛋白质组，定位翻译后 修饰和药物结合位点的膜蛋白受体，更准确和灵敏地 测量复杂组织和细胞裂解物中的差异蛋白质丰度，测量新合成的 通过激光捕获显微切割分离组织中的蛋白质，进行氢/氘交换 测量，并使用化学交联实验精制冷冻EM衍生的蛋白质结构。的 仪器将支持广泛的疾病的研究，包括癌症，白内障，青光眼，药物 成瘾和听力损失。该工具将使PSR能够将其服务扩展到越来越多的 大学里的蛋白质组学研究人员需要使用最先进的质谱仪， 以满足他们的研究需求。