Linear Ion Trap Mass Spectrometer

线性离子阱质谱仪

• 批准号: 6876906
• 负责人: LARRY L DAVID
• 金额: $ 30.45万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-08 至 2006-08-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876906
• 关键词: biomedical equipment purchase; electrospray ionization mass spectrometry

DESCRIPTION (provided by applicant): We propose the purchase of a ThermoFinnigan LTQ Linear Ion Trap Mass Spectrometer to place in an existing Ophthalmology Proteomics and Mass Spectrometry Core. This instrument is required because the LCQ Classic ion trap now used in the core no longer fulfills the needs of the on-going projects that require an instrument of greater sensitivity and rate of data acquisition. The new instrument will support the on-going research of both Ophthalmology Core sponsored projects, and a number of collaborative projects throughout Oregon Health & Science University. The instrument will be exclusively used for nano-electrospray ionization analysis of peptide digests to generate tandem mass spectral data for protein identification. An existing Agilent capillary liquid chromatography system will be used with the instrument to perform automated analysis in a high throughput environment. The current LCQ classic will be dedicated for use with an atmospheric pressure ionization MALDI source for analysis of simple digests from electrophoresis gels. Dedicating each instrument for either electrospray or MALDI analysis will greatly increase the efficiency of the core and increase productivity. The increased sensitivity and scan rates of the proposed linear trap will be used for complex peptide digests derived from protein/protein interaction studies and shotgun identification and quantification of proteins from tissue homogenates and body fluids. The proposed experiments using multidimensional chromatography are feasible because the core employs two full time bioinformatics specialists and a laboratory associate currently performing these types of experiments, and possessing a suite of software tools and hardware to handle the large data sets. This state-of-the-art instrument will be used in 11 diverse multi-disciplinary projects that will study the molecular causes of: cataract, glaucoma, uveitis, retinitis, corneal injury, cystic fibrosis, nephrotic syndrome, central nervous system dysfunction following exposure to toxins, suppression of apotosis, and glioblastomas. An additional use will be to study the composition of proteins in human saliva to test the usefulness of this fluid to detect biomarkers for disease.

描述（由申请人提供）：我们建议购买ThermoFinnigan LTQ线性离子阱质谱仪，以放置在现有的眼科蛋白质组学和质谱核心中。之所以需要这种仪器，是因为堆芯中现在使用的LCQ经典离子阱不再满足正在进行的项目的需要，这些项目需要更高灵敏度和数据采集速率的仪器。 新仪器将支持眼科核心赞助项目以及整个俄勒冈州健康与科学大学的许多合作项目的正在进行的研究。该仪器将专门用于肽序列的纳米电喷雾电离分析，以生成用于蛋白质鉴定的串联质谱数据。现有的Agilent毛细管液相色谱系统将与仪器一起使用，以在高通量环境中进行自动分析。目前的LCQ经典将致力于与大气压电离MALDI源用于分析简单的电泳凝胶。将每台仪器专用于电喷雾或MALDI分析将大大提高岩心的效率并提高生产率。 所提出的线性捕获器的增加的灵敏度和扫描速率将用于来自蛋白质/蛋白质相互作用研究和鸟枪鉴定的复杂肽序列，以及来自组织匀浆和体液的蛋白质的定量。使用多维色谱法的拟议实验是可行的，因为核心雇用了两名全职生物信息学专家和一名实验室助理，目前正在进行这些类型的实验，并拥有一套软件工具和硬件来处理大型数据集。 这种最先进的仪器将用于11个不同的多学科项目，这些项目将研究以下疾病的分子原因：白内障、青光眼、葡萄膜炎、视网膜炎、角膜损伤、囊性纤维化、肾病综合征、暴露于毒素后的中枢神经系统功能障碍、细胞凋亡抑制和胶质母细胞瘤。另一个用途是研究人类唾液中蛋白质的组成，以测试这种液体在检测疾病生物标志物方面的有用性。

项目成果

Stable liquid glucagon formulations for rescue treatment and bi-hormonal closed-loop pancreas.

• DOI: 10.1007/s11892-012-0320-5
• 发表时间: 2012-12
• 期刊: CURRENT DIABETES REPORTS
• 影响因子: 4.2
• 作者: Jackson, Melanie A.;Caputo, Nicholas;Castle, Jessica R.;David, Larry L.;Roberts, Charles T., Jr.;Ward, W. Kenneth
• 通讯作者: Ward, W. Kenneth