LTQ Orbitrap Velos

LTQ Orbitrap Velos

• 批准号: 8333650
• 负责人: LARRY L DAVID
• 金额: $ 85.37万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8333650
• 关键词: Alcoholism; Cataract; Chromatography; Complex Mixtures; Data; Detection; Deuterium; Disease; Dissociation; Electron Transport; Generations; Health Sciences; Hydrogen; Label; Measures; Methamphetamine dependence; Modification; Oregon; Parasitic Diseases; Peptides; Productivity; Proteins; Proteomics; Proteomics Shared Resource; Relative (related person); Research; Research Personnel; Resolution; Sampling; Scanning; Source; Speed; Structure; Trypsin; Universities; hearing impairment; human disease; improved; instrument; leukemia; mass spectrometer; protein complex; stable isotope; tool

DESCRIPTION (provided by applicant): We request a Thermo Scientific Orbitrap Elite mass spectrometer with an electron transfer dissociation source to be placed at the Oregon Health & Science University in the Proteomics Shared Resource. Due to its high resolution, sensitivity and fast scan rates, this instrument will be used to increase the productivity of proteomics research across the entire OHSU campus. The major users of the instrument utilize it to study the cause and treatment of several diseases including: cataracts, hearing loss, leukemia, parasitic disease, alcoholism, and methamphetamine addiction. Complex mixtures of proteins will be analyzed by digesting them with trypsin, separating the resulting peptides by chromatography and generation of tandem MS data. Quantification of relative changes in protein abundance between samples will be determined using both stable isotope tagging strategies and label free quantification. Modifications in proteins will be detected with the aid o the requested electron transfer dissociation source. The high resolution of the instrument will also be used to measure hydrogen/deuterium exchange rates in proteins to probe the structure of protein complexes. The introduction of this instrument will fulfill the growing needs of the researchers at the Oregon Health & Sciences University to incorporate state-of-the-art tools in proteomics into their research. This will speed their progress of discovery to improve the detection and treatment of human disease. PUBLIC HEALTH RELEVANCE: We request funding for a Thermo Scientific Orbitrap Elite high-resolution mass spectrometer. The instrument will be used to identify and determine the relative abundance of proteins to support research on diseases including cancer, cataracts, hearing loss, parasitic infection, and drug addiction. The instrument will be maximally utilized by placement in the Oregon Health & Science University's Proteomics Shared Research where its introduction will increase research productivity across the entire campus.

描述（由申请人提供）：我们请求将带有电子转移解离源的Thermo Scientific Orbitrap Elite质谱仪放置在俄勒冈州健康与科学大学的蛋白质组学共享资源中。由于其高分辨率，灵敏度和快速扫描速率，该仪器将用于提高整个OHSU校园的蛋白质组学研究的生产力。该仪器的主要用户利用它来研究几种疾病的原因和治疗方法，包括：白内障，听力损失，白血病，寄生虫病，酒精中毒和甲基苯丙胺成瘾。将通过用胰蛋白酶消化蛋白质的复杂混合物，通过色谱法分离所得肽并生成串联MS数据来分析蛋白质的复杂混合物。将使用稳定同位素标记策略和无标记定量测定样品之间蛋白质丰度的相对变化。蛋白质中的修饰将借助于所要求的电子转移解离源来检测。该仪器的高分辨率还将用于测量蛋白质中的氢/氘交换率，以探测蛋白质复合物的结构。该仪器的引入将满足俄勒冈州健康与科学大学研究人员日益增长的需求，将蛋白质组学中最先进的工具纳入他们的研究中。这将加速他们的发现进程，以改善人类疾病的检测和治疗。 公共卫生相关性：我们要求为Thermo Scientific Orbitrap Elite高分辨率质谱仪提供资金。该仪器将用于识别和确定蛋白质的相对丰度，以支持对癌症，白内障，听力损失，寄生虫感染和药物成瘾等疾病的研究。该工具将被最大限度地利用， 在俄勒冈州健康与科学大学的蛋白质组学共享研究中，它的引入将提高整个校园的研究生产力。