The signaling mechanisms of Arabidopsis CRY2

拟南芥CRY2信号传导机制

基本信息

批准号：
10264864
负责人：
CHENTAO LIN
金额：
$ 55.63万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10264864
关键词：
Address Adolescent Adult Affect Affinity Chromatography Age Animals Arabidopsis Bacteria Biological Models Biology Cells Chloroplasts Competence Complex Cues Development Embryo Funding Gene Expression Gene Expression Regulation Genes Genetic Genetic Transcription Germination Goals Histone Deacetylase Histone Deacetylation Homodimerization Human Laboratories Light Mammals Mass Spectrum Analysis Mediating Messenger RNA Methylation Methyltransferase MicroRNAs Molecular Nuclear Phase Transition Phosphorylation Photosensitivity Plants Polyubiquitination Post-Transcriptional RNA Processing Post-Transcriptional Regulation Process Protein Kinase Proteins RNA RNA methylation Reader Regulation Reporting Repressor Proteins Role Serine Signal Transduction System Testing Threonine Transcription Coactivator Transcription Repressor Transcriptional Regulation Ubiquitination age related base circadian pacemaker cryptochrome experimental study genetic analysis inhibitor/antagonist novel photoactivation photoperiodicity plant growth/development protein expression receptor recruit reproductive response transcription factor ubiquitin-protein ligase

项目摘要

Summary How cells respond to light and/or timing signal is a fundamental question in biology. Cryptochromes (CRYs) are evolutionarily conserved blue light receptors or the core circadian clock components found in all major evolutionary lineages, from bacteria to human. Our long-term goal is to understand the molecular mechanisms underlying cryptochrome signal transduction and regulation, using Arabidopsis CRY2 as a model system. Arabidopsis CRY2 is presently the best- studied plant cryptochrome. My laboratory has discovered most known aspects of the function and signal transduction mechanisms of CRY2. In the previous funding cycle, we discovered the photoactivation and regulatory mechanisms of CRY2. We found that photoexcited CRY2 undergoes homodimerization to become active; photoactivated CRY2 is inactivated by the Blue- light Inhibitors of Cryptochrome (BIC1 and BIC2). Photoactivated CRY2 is also phosphorylated by four Photoregulatory Protein Kinases (PPK1-4) on at least 24 serine and threonine residues. Phosphorylated CRY2 is not only fully active but also undergoes ubiquitination and degradation. However, several important questions of CRY2 signal transduction remain unsolved, including how CRY2 mediate light regulation of transcriptional and post-transcriptional changes of gene expression to modulate plant development and how CRY2 itself is regulated by light to modulate photosensitivity of plants. This renewal application attempt to address those questions based on results of our recent experiments.

概括细胞如何对光和/或定时信号的反应是生物学中的一个基本问题。加密色素（Crys）是进化保守的蓝光受体或核心昼夜节律时钟从细菌到人类的所有主要进化谱系中发现的成分。我们的长期目标是要了解加密信号转导的分子机制和调节，使用拟南芥CRY2作为模型系统。拟南芥Cry2目前是最好的研究了植物的隐性色原。我的实验室发现了功能的最著名方面 CRY2的信号转导机制。在上一个资金周期中，我们发现了 CRY2的光活化和调节机制。我们发现光激发Cry2 经历同二聚化以变得活跃；光活化的Cry2被蓝色灭活隐形色素的光抑制剂（BIC1和BIC2）。光活化的CRY2也被磷酸化至少24个丝氨酸和苏氨酸残基上的四个光子调节蛋白激酶（PPK1-4）。磷酸化的CRY2不仅是完全活跃的，而且会经历泛素化和降解。但是，Cry2信号转导的几个重要问题仍未解决，包括如何 CRY2介导基因转录和转录后变化的光调节调节植物发育的表达以及如何通过光调节Cry2本身调节植物的光敏性。这种续签申请试图基于我们最近实验的结果。

项目成果

期刊论文数量（51）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The cryptochromes.

DOI：
10.1186/gb-2005-6-5-220
发表时间：
2005
期刊：
Genome biology
影响因子：
12.3
作者：
通讯作者：

Phototropin blue light receptors and light-induced movement responses in plants.

植物中向光素蓝光受体和光诱导的运动反应。

DOI：
10.1126/stke.2002.118.pe5
发表时间：
2002
期刊：
Science's STKE : signal transduction knowledge environment
影响因子：
0
作者：
Lin,Chentao
通讯作者：
Lin,Chentao

The action mechanisms of plant cryptochromes.

DOI：
10.1016/j.tplants.2011.09.002
发表时间：
2011-12
期刊：
TRENDS IN PLANT SCIENCE
影响因子：
20.5
作者：
Liu, Hongtao;Liu, Bin;Zhao, Chenxi;Pepper, Michael;Lin, Chentao
通讯作者：
Lin, Chentao

Publisher Correction: A photoregulatory mechanism of the circadian clock in Arabidopsis.

出版商更正：拟南芥生物钟的光调节机制。

DOI：
10.1038/s41477-021-01027-4
发表时间：
2021
期刊：
Nature plants
影响因子：
18
作者：
Wang,Xu;Jiang,Bochen;Gu,Lianfeng;Chen,Yadi;Mora,Manuel;Zhu,Mulangma;Noory,Eliace;Wang,Qin;Lin,Chentao
通讯作者：
Lin,Chentao

Phytochromes and phytohormones: the shrinking degree of separation.