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The signaling mechanisms of Arabidopsis CRY2

拟南芥CRY2信号传导机制

基本信息

批准号：
10264864
负责人：
CHENTAO LIN
金额：
$ 55.63万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10264864
关键词：
Address Adolescent Adult Affect Affinity Chromatography Age Animals Arabidopsis Bacteria Biological Models Biology Cells Chloroplasts Competence Complex Cues Development Embryo Funding Gene Expression Gene Expression Regulation Genes Genetic Genetic Transcription Germination Goals Histone Deacetylase Histone Deacetylation Homodimerization Human Laboratories Light Mammals Mass Spectrum Analysis Mediating Messenger RNA Methylation Methyltransferase MicroRNAs Molecular Nuclear Phase Transition Phosphorylation Photosensitivity Plants Polyubiquitination Post-Transcriptional RNA Processing Post-Transcriptional Regulation Process Protein Kinase Proteins RNA RNA methylation Reader Regulation Reporting Repressor Proteins Role Serine Signal Transduction System Testing Threonine Transcription Coactivator Transcription Repressor Transcriptional Regulation Ubiquitination age related base circadian pacemaker cryptochrome experimental study genetic analysis inhibitor/antagonist novel photoactivation photoperiodicity plant growth/development protein expression receptor recruit reproductive response transcription factor ubiquitin-protein ligase

项目摘要

Summary How cells respond to light and/or timing signal is a fundamental question in biology. Cryptochromes (CRYs) are evolutionarily conserved blue light receptors or the core circadian clock components found in all major evolutionary lineages, from bacteria to human. Our long-term goal is to understand the molecular mechanisms underlying cryptochrome signal transduction and regulation, using Arabidopsis CRY2 as a model system. Arabidopsis CRY2 is presently the best- studied plant cryptochrome. My laboratory has discovered most known aspects of the function and signal transduction mechanisms of CRY2. In the previous funding cycle, we discovered the photoactivation and regulatory mechanisms of CRY2. We found that photoexcited CRY2 undergoes homodimerization to become active; photoactivated CRY2 is inactivated by the Blue- light Inhibitors of Cryptochrome (BIC1 and BIC2). Photoactivated CRY2 is also phosphorylated by four Photoregulatory Protein Kinases (PPK1-4) on at least 24 serine and threonine residues. Phosphorylated CRY2 is not only fully active but also undergoes ubiquitination and degradation. However, several important questions of CRY2 signal transduction remain unsolved, including how CRY2 mediate light regulation of transcriptional and post-transcriptional changes of gene expression to modulate plant development and how CRY2 itself is regulated by light to modulate photosensitivity of plants. This renewal application attempt to address those questions based on results of our recent experiments.

概括细胞如何响应光和/或定时信号是生物学中的一个基本问题。隐花色素 (CRY) 是进化上保守的蓝光受体或核心生物钟从细菌到人类的所有主要进化谱系中都发现了这些成分。我们的长期目标是了解隐花色素信号转导的分子机制调节，使用拟南芥 CRY2 作为模型系统。拟南芥 CRY2 是目前最好的- 研究了植物隐花色素。我的实验室已经发现了该功能最知名的方面 CRY2的信号转导机制。在上一个融资周期中，我们发现 CRY2的光激活和调控机制。我们发现光激发的CRY2 经历同二聚化变得活跃；光激活的 CRY2 被 Blue- 灭活隐花色素（BIC1 和 BIC2）的光抑制剂。光激活的 CRY2 也被磷酸化至少 24 个丝氨酸和苏氨酸残基上有四种光调节蛋白激酶 (PPK1-4)。磷酸化的 CRY2 不仅具有完全活性，而且还会发生泛素化和降解。然而，CRY2信号转导的几个重要问题仍未解决，包括如何 CRY2介导基因转录和转录后变化的光调节表达调节植物发育以及CRY2本身如何受光调节来调节植物的光敏感性。此续订申请试图根据以下内容解决这些问题我们最近的实验结果。

项目成果

期刊论文数量（51）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The cryptochromes.

DOI：
10.1186/gb-2005-6-5-220
发表时间：
2005
期刊：
Genome biology
影响因子：
12.3
作者：
通讯作者：

The action mechanisms of plant cryptochromes.

DOI：
10.1016/j.tplants.2011.09.002
发表时间：
2011-12
期刊：
TRENDS IN PLANT SCIENCE
影响因子：
20.5
作者：
Liu, Hongtao;Liu, Bin;Zhao, Chenxi;Pepper, Michael;Lin, Chentao
通讯作者：
Lin, Chentao

Publisher Correction: A photoregulatory mechanism of the circadian clock in Arabidopsis.

出版商更正：拟南芥生物钟的光调节机制。

DOI：
10.1038/s41477-021-01027-4
发表时间：
2021
期刊：
Nature plants
影响因子：
18
作者：
Wang,Xu;Jiang,Bochen;Gu,Lianfeng;Chen,Yadi;Mora,Manuel;Zhu,Mulangma;Noory,Eliace;Wang,Qin;Lin,Chentao
通讯作者：
Lin,Chentao

Phototropin blue light receptors and light-induced movement responses in plants.

植物中向光素蓝光受体和光诱导的运动反应。