Plant Photosensory Receptor CRY2 Signaling Mechanism

植物光敏受体 CRY2 信号机制

• 批准号: 6382820
• 负责人: CHENTAO LIN
• 金额: $ 31.06万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6382820
• 关键词: Arabidopsis; biological signal transduction; chimeric proteins; circadian rhythms; corticosteroid receptors; gene expression; gene mutation; genetic mapping; genetic markers; molecular cloning; nonvisual photoreceptor; plant genetics; plant growth /development; plant proteins; polymerase chain reaction; protein protein interaction; protein structure function; receptor expression; subtraction hybridization; western blottings; yeast two hybrid system

DESCRIPTION (provided by applicant): The ability to sense and respond to the changing light environment is a fundamental feature of living organisms. The blue/UV-A light receptor cryptochromes is the only photoreceptor that functions in both plants and animals to regulate photoperiodic responses and the circadian clock, which have been associated with not only plant development, but also various human health problems including sleeping disorders and behavioral disorders. We have recently discovered that Arabidopsis cry2 is a major photoreceptor controlling photoperiodic flowering in plants. We have also found that a calcium-binding protein SUB1 is one of the downstream signaling components of cry2. We hypothesize that protein-protein interaction is associated with the early signaling process of cry2 and that regulation of gene expression is a major mechanism underlying cry2 function. We propose to continue investigate the signal transduction mechanism of Arabidopsis cry2. The objectives of this proposal are: (1) to identify and characterize the genetic suppressors of the sub1 mutant, and to clone and characterize the SUB4 gene and test a hypothesis that SUB4 may act as a negative regulator of cry2. (2) to investigate how cry2 regulates photoperiodic flowering by identifying genes for which the expression is regulated by cry2. (3) to continue characterize a cry2-interacting protein that we have recently isolated, and to isolate and characterize additional cry2-interactinig proteins. (4) to isolate and characterize Arabidopsis mutants impaired in blue light-induced cry2 degradation and blue light-dependent induction of transcription.

描述（由申请人提供）：感知和响应的能力 光环境的变化是生物体的基本特征。的 蓝/UV-A光受体隐花色素是唯一起作用的光感受器 在植物和动物中调节光周期反应， 生物钟不仅与植物的发育有关， 还包括各种人类健康问题，包括睡眠障碍， 行为障碍我们最近发现，拟南芥cry 2是一种 植物中控制光周期开花的主要光感受器。我们还 发现钙结合蛋白SUB1是下游信号传导之一， Cry 2的成分。我们假设蛋白质-蛋白质相互作用是 与cry 2的早期信号传导过程和基因调控有关， 表达是cry 2功能的主要机制。我们建议 继续研究拟南芥cry 2的信号转导机制。的 该提案的目标是：（1）识别和表征遗传 抑制子的sub1突变体，并克隆和表征SUB 4基因， 测试SUB 4可能作为cry 2负调节因子的假设。(2)到 研究cry 2如何通过鉴定基因来调节光周期开花 其表达受cry 2调控。(3)继续描述 cry 2相互作用蛋白，我们最近分离，并分离和 表征另外的cry 2相互作用蛋白。(4)分离和 表征在蓝光诱导的cry 2中受损的拟南芥突变体 降解和蓝光依赖的转录诱导。