PLANT PHOTOSENSORY RECEPTOR CRY2--SIGNALING MECHANISM

植物光敏受体CRY2--信号机制

• 批准号: 2383406
• 负责人: CHENTAO LIN
• 金额: $ 10.64万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2383406
• 关键词: Arabidopsis; biological signal transduction; gene mutation; nonvisual photoreceptor; plant genetics; plant proteins; polymerase chain reaction; protein structure function; receptor expression; yeast two hybrid system

The ability to perceive and respond to the changing environment of solar radiation is a fundamental feature of most living organisms. Flavin-type blue light receptors known as cryptochrome is a major group of photosensory receptors which mediate various blue light responses in higher plants. Despite the recent progress made in the identification of this type of photoreceptors, our understanding with respect to the molecular mechanisms by which a cryptochrome mediates plant response to blue light is very limited. The proposed research is based on our recent isolation and characterization of the Arabidopsis gene CRY2, the second member of the Arabidopsis cryptochrome gene family. CRY2 is unique not only because it appears to mediate different blue light responses from that regulated by CRY1, but also in that its own expression is regulated by blue light. The long term goal of our study is to understand how plants respond to blue light, and we will take the combined approaches of biochemistry, molecular biology, and genetics to achieve the specific aims of this proposal. We propose to characterize the biochemical properties of the CRY2 protein purified from either homologous- or heterologous- expression systems. We will also identify the mechanism(s) responsible for the blue light-induced regulation of CRY2 expression. The biological function of CRY2 will be investigated by isolation and characterization of Arabidopsis cry2 mutants impaired in the CRY2 structure gene using different genetic methods. Accomplishment of these specific aims will allow experimental dissection of the molecular mechanisms underlying the ability of CRY2 to mediate blue light responses, and it will make it possible to gain further understanding of a vital signal transduction process in eucaryotic organisms.

感知和应对环境变化的能力 太阳辐射是大多数生物体的基本特征。 黄素型蓝光受体被称为隐花色素是一种主要的 一组感光受体，可调节各种蓝光 高等植物的反应。 尽管最近取得了进展， 识别这种类型的光感受器，我们的理解与 关于隐花色素介导 植物对蓝光的反应非常有限。 拟议研究 是基于我们最近的隔离和表征的 拟南芥属基因f12，拟南芥属的第二个成员， 隐花色素基因家族 1992年是独一无二的，不仅因为它似乎 为了介导与由BMP1调节的蓝光反应不同的蓝光反应， 还因为它自身的表达受蓝光调节。 的 我们研究的长期目标是了解植物如何应对 蓝光，我们将采取生物化学的综合方法， 分子生物学和遗传学，以实现这一特定目标， 提议 我们建议表征的生化特性， 纯化自同源或异源蛋白质的HSP12蛋白质， 表达系统。 我们还将确定机制 负责蓝光诱导的BMP2表达的调节。 将通过分离和鉴定来研究BMP2的生物学功能， 拟南芥cry2突变体的表征， 用不同的遗传学方法构建基因。 完成 这些特定的目标将允许实验解剖 BMP2介导蓝色的分子机制 光的反应，这将使它有可能获得进一步的 了解真核细胞中一个重要的信号转导过程 有机体