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Plant photoreceptor CRY2 and Signaling Mechanism

植物光感受器CRY2及其信号机制

基本信息

批准号：
9275106
负责人：
CHENTAO LIN
金额：
$ 1.46万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9275106
关键词：
Address Animals Arabidopsis Bacteria Binding Biological Models Biology Circadian Rhythms Code Complex Core Protein DNA Sequence Electrons Elements F Box Domain F-Box Proteins Flowers Funding Gene Expression Genes Genetic Transcription Genetic study Goals Health Human In Vitro Investigation Laboratories Lasers Light Mediating Messenger RNA Methodology MicroRNAs Molecular Mutagenesis Organism Oxidation-Reduction Phosphorylation Photochemistry Photoreceptors Phototransduction Plant Photoreceptors Plants Post-Transcriptional Regulation Post-Translational Regulation Proteins Proteolysis Protons RNA Sequences RNA-Binding Proteins Reaction Regulation Signal Transduction Site Testing Transgenes Triad Acrylic Resin Untranslated Regions Work base biophysical analysis chromophore circadian pacemaker cryptochrome driving force in vivo inhibitor/antagonist light entrainment mRNA Expression mutant novel phyA phytochrome plant growth/development protein complex protein degradation receptor response tool

项目摘要

DESCRIPTION (provided by applicant): How organisms respond to light and how photosensory receptors mediate light responses are basic questions in biology. Our long-term goal is to find the molecular explanation of these questions, using the cryptochrome photoreceptor as a model system. Cryptochromes (CRY) are the blue/UV-A light receptors and/or core components of the circadian oscillator found in all evolutionary lineages including human. My laboratory focuses on the study of plant cryptochromes. In the previous funding periods, we discovered two major CRY2 signal transduction mechanisms: the CIB1 (Cryptochrome-Interacting bHLH 1)-based transcription- regulatory mechanism and SPA1/COP1 (Suppressor of Phytochrome A 1/Constitutive Photomorphogenesis 1)-based proteolysis-regulatory mechanism. More recently, we identified a CIB-interacting protein FOF1 (F-box of Flowering 1) responsible for the blue light-regulated degradation of CIB1; two new blue light-specific CRY2-interacting proteins: PRR5 (Pseudo Response Regulator 5) that is a core protein of the circadian clock, and a novel protein BIC1 (Blue- light Inhibitor of CRYs 1) tha suppresses blue light-dependent phosphorylation, degradation, and activities of CRY1 and CRY2. Based on these discoveries and newly developed tools, we propose to study three key issues of light signal transduction: the photochemical mechanism underlying photoexcitation of the CRY photoreceptor, mechanisms governing the function and regulation of the CRY complexome, and the mechanism underlying a novel coding sequence (CDS)-dependent blue light regulation of CRY2 expression.

描述（由申请人提供）：生物体如何响应光以及光感应受体如何介导光响应是生物学中的基本问题。我们的长期目标是使用隐花色素光感受器作为模型系统，找到这些问题的分子解释。隐花色素 (CRY) 是蓝色/UV-A 光受体和/或昼夜节律振荡器的核心成分，存在于包括人类在内的所有进化谱系中。我的实验室专注于植物隐花色素的研究。在之前的资助期间，我们发现了两种主要的CRY2信号转导机制：基于CIB1（Cryptochrome-Interacting bHLH 1）的转录调节机制和基于SPA1/COP1（Suppressor of Phytochrome A 1/Constitutive Photomorphogenesis 1）的蛋白水解调节机制。最近，我们发现了一种 CIB 相互作用蛋白 FOF1（F-box of Flowering 1）负责蓝光调节的 CIB1 降解；两种新的蓝光特异性 CRY2 相互作用蛋白：PRR5（伪响应调节器 5）（生物钟的核心蛋白）和新型蛋白 BIC1（CRYs 1 蓝光抑制剂）可抑制蓝光依赖性磷酸化、降解以及 CRY1 和 CRY2 的活性。基于这些发现和新开发的工具，我们建议研究光信号转导的三个关键问题：CRY光感受器光激发的光化学机制、控制CRY复合体功能和调节的机制，以及CRY2表达的新型编码序列（CDS）依赖的蓝光调节的机制。