Plant Photosensory Receptor CRY2 Signaling Mechanism

描述（由申请人提供）：生物体如何对光作出反应以及光敏感受器如何介导光反应是生物学中影响人类健康的一些最基本的问题。隐色素（cryes）是一种存在于细菌、植物和动物（包括人类）中的蓝光/UV-A光受体，但其分子机制尚不清楚。我的实验室以拟南芥为模型系统，研究CRY1和CRY2调控植物发育的信号机制。我们最近首次在植物中发现了依赖蓝光的cry相互作用蛋白。我们提出继续研究CRY1和CRY2的信号机制有三个具体目标。首先，我们建议研究哭泣相互作用蛋白如何调节转录、mRNA输出和蛋白质稳定性。其次，我们计划研究哭泣对细胞类型特异性基因表达变化的调节。第三，我们提出鉴定和表征影响蓝光诱导的CRY2降解和蓝光抑制的CIB1降解的基因。公共卫生相关性：隐色素（CRY）是蓝色/UV-A光受体，调节植物发育和人类健康。我们建议研究CRY相互作用蛋白，以及它们如何介导CRY对光响应的基因表达调控。

DESCRIPTION (provided by applicant): How organisms respond to light and how photosensory receptors mediate light responses are some of the most basic questions in biology that affect human health. Cryptochromes (CRY) are blue/UV-A light receptors found in bacteria, plants, and animals including human, but the molecular mechanisms of CRYs remain not well understood. My laboratory uses Arabidopsis as the model system to study signaling mechanisms underlying CRY1 and CRY2 regulation of plant development. We recently identified, for the first time, blue light-dependent CRY-interacting proteins in plants. We propose to continue study CRY1 and CRY2 signaling mechanisms with three specific aims. First, we propose to investigate how CRY-interacting proteins regulate transcription, mRNA export, and protein stability. Second, we plan to investigate CRY regulation of cell-type- specific gene expression changes. Third, we propose to identify and characterize genes affecting blue light-induced CRY2 degradation and blue light- suppressed CIB1 degradation. PUBLIC HEALTH RELEVANCE: Cryptochromes (CRY) are blue/UV-A light receptors regulating plant development and human health. We propose to study CRY-interacting proteins and how they mediate CRY regulation of gene expression in response to light.