Comprehensive linking of DNA Elements in high-priority ENCODE Biosamples to their promoter targets

摘要。 人类基因组中大约两米长的DNA被复杂地包装成染色质， 每个细胞核中的染色体。除了其结构性作用外，该组织还具有重要的监管职能， 功能协调发展的特别是，人类基因组中环的形成在调节基因中起着至关重要的作用。 我们最近展示了使用原位Hi-C方法创建这些循环的可靠地图的能力， 三维基因组测序。Hi-C表征了基因组的三维结构 通过测定基因组范围内所有基因座对之间的物理接触频率。拟议的中心 将应用Hi-C和其他新技术来表征基因组环，它们的调节和功能。 我们将专门研究各种ENCODE细胞类型中的这些结构。推导出的原理 将适用于任何哺乳动物组织类型。

ABSTRACT. The roughly two meters of DNA in the human genome is intricately packaged to form the chromatin and chromosomes in each cell nucleus. In addition to its structural role, this organization has critical regulatory functions. In particular, the formation of loops in the human genome plays an essential role in regulating genes. We recently demonstrated the ability to create reliable maps of these loops, using an in situ Hi-C method for three-dimensional genome sequencing. Hi-C characterizes the three- dimensional configuration of the genome by determining the frequency of physical contact between all pairs of loci, genome-wide. The proposed center will apply Hi-C and other new technologies to characterize genomic loops, their regulation, and their functions. We will specifically examine these structures in a wide variety of ENCODE cell types. The principles deduced from our study will be applicable to any mammalian tissue type.