Macromolecular Crystallography Research with Synchrotron Radiation
同步辐射高分子晶体学研究
基本信息
- 批准号:10262078
- 负责人:
- 金额:$ 128.19万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:AgricultureAmino AcidsAnabolismAnimalsBiologicalCharacteristicsCollaborationsCommunitiesCrystallizationCrystallographyDataDevelopmentEnzymesFabaceaeHistidineHuman ResourcesInvestigationLaboratoriesLigandsLigaseLiteratureMeasuresMedicago truncatulaMedicalMethodologyMolecularPathway interactionsPharmacologyPhasePhotonsPlant ModelPlantsPolyaminesPropertyProteinsPublishingPutrescineRadiation induced damageReactionReagentResearchResearch PersonnelRestRoentgen RaysSeleniumSerineSignal TransductionSourceSpecimenSpermidineSpermidine SynthaseSpermineStructural ProteinStructureSynchrotronsTwin Multiple BirthUnited States National Institutes of HealthWorkZ-Form DNAdata warehouseexperimental studymacromoleculenovelpathogenprotein complexsynchrotron radiation
项目摘要
During this year we have shown the practical usefulness of selenourea, the reagent that can be soaked into the native crystals of proteins to provide the strong anomalous diffraction signal of selenium used for solving novel crystal structures of macromolecules. Selenourea was in the previous year proposed to the biocrystallographic community by us as a simple and powerful vehicle for phasing diffraction data measured at synchrotron X-ray beam lines. This reagent allowed us to solve the crystal structure of XXX, one of the enzymes from the biosynthetic pathway of the amino acid histidine in plants. Within the methodological aspects of our activities, we conducted the analysis of non-crystallographic screw axes of different types occurring in protein crystal structures stored in the Protein Data Bank (PDB). In collaboration with Dr. Wlodawer (MCL in Frederick) and other colleagues the analysis various complexes of proteins with small molecular ligands was thoroughly analyzed and validated, often correcting the original suboptimal interpretation of results available in the literature and in the PDB. The unusual crystal structures of Z-DNA were analyzed, and interpreted as highly pseudosymmetric and six-fold merohedrally twinned specimens, not observed previously for these types of crystals. Within our activity directed at solving and interpreting crystal structures of proteins having biological or medical importance, we worked on selected model plant enzymes from the biosynthetic pathways of amino acids serine and histidine as well as aliphatic polyamines, which are not synthesized in animals and therefore may be relevant for development of agents active against various pathogens, with potential application in pharmacology or agriculture. The histidine pathway in the legume plant Medicago truncatula contain seven enzymes, catalyzing several reactions (some are bifunctional). All of them are expressed and five crystal structures are solved, interpreted and recently published. The rest are crystallized and the work on them continues. Four enzymes from the serine pathway are obtained and crystallized and structures of two of them fully characterized and published. The crystal structures of several enzymes involved in biosynthesis of polyamines putrescine spermine, spermidine and thermospermine were recently solved. The interpretation of the structure of thermospermine synthetase is published and work on other aminopropyltransferases continues.
在这一年中,我们已经展示了硒的实用性,该试剂可以浸入蛋白质的天然晶体中,以提供硒的强烈异常衍射信号,用于解决大分子的新晶体结构。硒是在前一年提出的生物化学的allographic社区由我们作为一个简单而强大的车辆相位衍射数据在同步加速器X射线光束线测量。这种试剂使我们能够解决XXX的晶体结构,XXX是植物中氨基酸组氨酸生物合成途径中的酶之一。在我们活动的方法学方面,我们对存储在蛋白质数据库(PDB)中的蛋白质晶体结构中发生的不同类型的非晶体学螺旋轴进行了分析。与Wlodawer博士(弗雷德里克的MCL)和其他同事合作,对蛋白质与小分子配体的各种复合物进行了彻底的分析和验证,经常纠正文献和PDB中对结果的原始次优解释。Z-DNA的不寻常的晶体结构进行了分析,并解释为高度假对称和六重半面体孪生标本,以前没有观察到这些类型的晶体。在我们致力于解决和解释具有生物学或医学重要性的蛋白质的晶体结构的活动中,我们研究了从氨基酸丝氨酸和组氨酸以及脂肪族多胺的生物合成途径中选择的模型植物酶,这些酶在动物中不合成,因此可能与开发对各种病原体具有活性的药物相关,并在药理学或农业中具有潜在的应用。豆科植物蒺藜苜蓿(Medicago truncatula)中的组氨酸途径包含七种酶,催化几种反应(有些是双功能的)。所有这些都表达和五个晶体结构的解决,解释和最近发表。其余的都是结晶,对它们的工作仍在继续。从丝氨酸途径的四种酶获得和结晶,其中两种的结构充分表征和出版。多胺、腐胺、精胺、亚精胺和热精胺生物合成中涉及的几种酶的晶体结构最近得到了解决。热精胺合成酶的结构的解释出版和其他氨丙基转移酶的工作继续进行。
项目成果
期刊论文数量(69)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.
具有基于色氨酸的发色团的光毒性橙色荧光蛋白的晶体结构。
- DOI:10.1371/journal.pone.0145740
- 发表时间:2015
- 期刊:
- 影响因子:3.7
- 作者:Pletneva NV;Pletnev VZ;Sarkisyan KS;Gorbachev DA;Egorov ES;Mishin AS;Lukyanov KA;Dauter Z;Pletnev S
- 通讯作者:Pletnev S
Collection of X-Ray Diffraction Data from Macromolecular Crystals.
- DOI:10.1007/978-1-4939-7000-1_7
- 发表时间:2017
- 期刊:
- 影响因子:0
- 作者:Dauter Z
- 通讯作者:Dauter Z
On methylene-bridged cysteine and lysine residues in proteins.
关于蛋白质中亚甲基桥半胱氨酸和赖氨酸残基。
- DOI:10.1002/pro.2958
- 发表时间:2016
- 期刊:
- 影响因子:0
- 作者:Ruszkowski,Milosz;Dauter,Zbigniew
- 通讯作者:Dauter,Zbigniew
Sulfur-SAD phasing from microcrystals utilizing low-energy X-rays.
利用低能 X 射线对微晶进行硫-SAD 定相。
- DOI:10.1107/s2052252519008698
- 发表时间:2019
- 期刊:
- 影响因子:3.9
- 作者:Dauter,Zbigniew
- 通讯作者:Dauter,Zbigniew
Missed opportunities in crystallography.
错过了晶体学的机会。
- DOI:10.1111/febs.12832
- 发表时间:2014
- 期刊:
- 影响因子:0
- 作者:Dauter,Zbigniew;Jaskolski,Mariusz
- 通讯作者:Jaskolski,Mariusz
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{{ truncateString('ZBIGNIEW DAUTER', 18)}}的其他基金
ANALYSIS OF CRYSTAL STRUCTURES AT VERY HIGH RESOLUTION
非常高分辨率的晶体结构分析
- 批准号:
8361715 - 财政年份:2011
- 资助金额:
$ 128.19万 - 项目类别:
CRYSTAL STRUCTURE OF HUMAN RHOA GDP RHOGDI COMPLEX & ITS BIOLOGICAL IMPLICATIONS
人类 RHOA GDP RHOGDI 复合物的晶体结构
- 批准号:
6205783 - 财政年份:1999
- 资助金额:
$ 128.19万 - 项目类别:
ANOMALOUS SIGNAL OF SULFUR AS TOOL FOR SOLVING PROTEIN CRYSTAL STRUCTURES?
硫的异常信号可作为解析蛋白质晶体结构的工具?
- 批准号:
6205782 - 财政年份:1999
- 资助金额:
$ 128.19万 - 项目类别:
CCD DETECTOR OPERATION FOR PROTEIN CRYSTALLOGRAPHY ON X9B
X9B 上蛋白质晶体学的 CCD 探测器操作
- 批准号:
6205771 - 财政年份:1999
- 资助金额:
$ 128.19万 - 项目类别:
ANOMALOUS SIGNAL OF SOLVENT BROMIDES USED FOR PHASING OF LYSOZYME
用于溶菌酶定相的溶剂溴化物的异常信号
- 批准号:
6205781 - 财政年份:1999
- 资助金额:
$ 128.19万 - 项目类别:
SYNCHROTRON CRYSTALLOGRAPHY OF ENZYMES AT BEAMLINE X 9B: HIV, DRUG TARGET
光束线 X 9B 上酶的同步晶体学:HIV、药物靶标
- 批准号:
6120424 - 财政年份:1998
- 资助金额:
$ 128.19万 - 项目类别:
SYNCHROTRON CRYSTALLOGRAPHY OF PROTEINS AT BEAMLINE X 9B
束线 X 9B 上蛋白质的同步加速器晶体学
- 批准号:
6120423 - 财政年份:1998
- 资助金额:
$ 128.19万 - 项目类别:
Macromolecular Crystallography Research with Synchrotron
同步加速器高分子晶体学研究
- 批准号:
7338501 - 财政年份:
- 资助金额:
$ 128.19万 - 项目类别:
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