Consortium for Viral Systems Biology Technology Core

病毒系统生物学技术核心联盟

• 批准号: 10579084
• 负责人: Bryan Briney
• 金额: $ 7.5万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10579084
• 关键词: Acute; Adaptive Immune System; Antibodies; Antibody Repertoire; Antigens; Area; B-Lymphocytes; Binding; Biological Assay; CD8-Positive T-Lymphocytes; Categories; Cell Separation; Cells; Clinical; Communicable Diseases; Communities; Custom; Data; Development; Disease Outbreaks; Disease Outcome; Ebola Hemorrhagic Fever; Ebola virus; Emerging Technologies; Ensure; Evaluation; Generations; Genetic study; Goals; High-Throughput Nucleotide Sequencing; Human; Human Resources; Immune response; Immunologic Receptors; Infection; Label; Lassa Fever; Lassa virus; Mass Spectrum Analysis; Memory; Memory B-Lymphocyte; Methodology; Methods; Mission; Molecular; Monoclonal Antibodies; Outcome; Plasma; Plasmablast; Protocols documentation; Records; Reproducibility; Role; Serology; Serum; Source; Standardization; System; Systems Biology; T-cell receptor repertoire; Techniques; Technology; Translating; Update; Viral; Viral Genome; Viral Hemorrhagic Fevers; Virus; Virus Diseases; Work; adaptive immune response; cost; design; falls; genome sequencing; high throughput screening; human leukocyte antigen testing; improved; innovation; longitudinal analysis; member; neutralizing antibody; next generation sequencing; novel; pathogen; seropositive; success; technology development; tool

Project Summary / Abstract  Viral hemorrhagic fevers, of which Ebola virus (EBOV) and Lassa virus (LASV) are prototypical examples, are a  major  source  of  human  suffering.  Recent  outbreaks  have  raised  concerns  about  the  possibility  of  a  global  outbreak. The ultimate goal of the Consortium for Viral Systems Biology (CViSB) is to define networks of host  and  viral  factors  that  drive  clinical  outcomes  in  Ebola  virus  disease  (EVD)  and  Lassa  fever  (LF).  The  responsibilities  and  contributions  of  the  Technology  Core  fall  into  three  main  categories:  data  generation,  technology  development  and  standardization,  and  evaluation  and  incorporation  of  emerging  technologies.  Although these categories are distinct, they are tightly integrated. The Core’s role as data generation hub for  CViSB  also  mandates  that  we  continually  work  to  develop  and  improve  our  methods  and  to  rapidly  incorporate novel technological advances to ensure that we remain a state-of-the-art, productive member of  the Center.   The  Technology  Core’s  Aims  are  focused  on the development, adaptation and standardization of innovative  experimental  approaches  in  four  main  areas:  (1)  deep  characterization  of  the adaptive immune response to  infection,  (2)  functional  profiling  of  antiviral  serum  activity,  (3)  isolation  and  characterization  of  protective  monoclonal antibodies and (4) cost-efficient viral genome sequencing. In addition to supporting the Center’s  Projects and contributing to the overall success of CViSB, the Technology Core will translate its  technical and  methodological  advances  into  standardized,  reproducible  protocols  that  will  benefit  other  Centers  and  the  broader infectious disease community.

项目摘要/摘要